Human Pref-1/DLK1/FA1 DuoSet ELISA

R&D Systems | Catalog # DY1144-05

R&D Systems
100% Guarantee

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

93.8-6000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human Pref-1/DLK1/FA1 DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all Pref-1/DLK1/FA1 ELISA Kits »

Product Summary for Human Pref-1/DLK1/FA1 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Pref-1 / DLK1 / FA1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human Pref-1/DLK1/FA1 DuoSet ELISA

Human Pref-1 / DLK1 / FA1 ELISA Standard Curve

Human Pref-1 / DLK1 / FA1 ELISA Standard Curve

Kit Contents for Human Pref-1/DLK1/FA1 DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)

Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)

Microplates: (Catalog # DY990), or equivalent

Plate Sealers: (Catalog # DY992), or equivalent

*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Pref-1/DLK1/FA1

Pref-1 (preadipocyte factor 1), also known as deltalike 1 (DLK1) and fetal antigen 1 (FA1), is a member of the Notch/Delta/Serrate protein family. It is a transmembrane protein that can be proteolyically cleaved to generate soluble forms. Pref-1 is believed to be a co-ligand for Notch signaling, enhancing or inhibiting Notch activation following binding of a ligand. It is expressed in numerous endocrine cells, and is highly expressed in preadipocytes, but not in mature adipocytes. In adipose tissue, Pref-1 inhibits adipogenesis and is often used as a preadipocyte marker.

Long Name

Preadipocyte Factor-1/Protein delta Homolog 1/Fetal Antigen 1

Alternate Names

DLK-1, DLK1, FA1, pG2, Pref1, ZOG

Entrez Gene IDs

8788 (Human); 13386 (Mouse)

Gene Symbol

DLK1

Additional Pref-1/DLK1/FA1 Products

Product Documents for Human Pref-1/DLK1/FA1 DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Pref-1/DLK1/FA1 DuoSet ELISA

For research use only

Citations for Human Pref-1/DLK1/FA1 DuoSet ELISA

Customer Reviews for Human Pref-1/DLK1/FA1 DuoSet ELISA

There are currently no reviews for this product. Be the first to review Human Pref-1/DLK1/FA1 DuoSet ELISA and earn rewards!

Have you used Human Pref-1/DLK1/FA1 DuoSet ELISA?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for ELISA Kits