Ig Kappa Light Chain Antibody (2495C)

R&D Systems | Catalog # MAB100502

Recombinant Monoclonal Antibody.
R&D Systems
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Key Product Details

Species Reactivity

Human, Primate

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2495C
View all Ig Kappa Light Chain Primary Antibodies »

Product Specifications

Immunogen

Human Ig Kappa Light Chain

Specificity

Detects human Ig Kappa Light Chain in direct ELISAs. Detects human and primate Ig Kappa Light Chain in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ig Kappa Light Chain Antibody (2495C)

Detection of Human and Primate Ig Kappa Light Chain antibody by Western Blot.

Detection of Human and Primate Ig Kappa Light Chain by Western Blot.

Western blot shows human kappa light chain protein, human lambda light chain protein (negative control), human serum, primate serum, mouse serum (negative control), and rat serum (negative control). PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Primate Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB100502) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Ig Kappa Light Chain at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Ig Kappa Light Chain Antibody (2495C)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human kappa light chain protein, Human serum, and Primate serum

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ig Kappa Light Chain

The immunoglobulin light chain is the smaller subunit of an antibody and in humans can be expressed in two types: as a kappa ( kappa ) or a lambda ( lambda ) chain.  Antibodies are produced by B-Cells that are clonal and each expresses only one type of light chain. The light chain class remains fixed for the life of the B lymphocyte. The ratio of kappa and lambda light chains can be used to determine disease status by immunohistochemistry or ELISA based assays.

Long Name

Immunoglobulin Kappa Light Chain

Alternate Names

ig kappa chain c region;Immunoglobulin K;immunoglobulin kappa light chain;Kappa LC

Additional Ig Kappa Light Chain Products

Product Documents for Ig Kappa Light Chain Antibody (2495C)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Ig Kappa Light Chain Antibody (2495C)

For research use only

Citations for Ig Kappa Light Chain Antibody (2495C)

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