Human/Primate Ig Lambda Light Chain Antibody

Catalog #: MAB100492 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB100492-100
MAB100492-SP

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Detection of Human and Primate Ig Lambda Light Chain by Western Blot.

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Human/Primate Ig Lambda Light Chain Antibody Summary

Species Reactivity

Human, Primate

Specificity

Detects human Ig Lambda Light Chain in direct ELISAs. Detects human and primate Ig Lambda Light Chain in Western blots.

Source

Monoclonal Rabbit IgG Clone # 2496A

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human Ig Lamda Light Chain

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Primate Ig Lambda Light Chain by Western Blot. Western blot shows lysates of human kappa light chain purified protein (negative sample), human lambda light chain purified protein, human serum, primate serum, mouse serum (negative sample), and rat serum (negative sample). PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Primate Ig Lambda Light Chain Monoclonal Antibody (Catalog # MAB100492) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Ig Lambda Light Chain at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ig Lambda Light Chain

The immunoglobulin light chain is the smaller subunit of an antibody and in humans can be expressed in two types: as a kappa ( kappa ) or a lambda ( lambda ) chain. Antibodies are produced by B-Cells that are clonal and each expresses only one type of light chain. The light chain class remains fixed for the life of the B lymphocyte. The ratio of kappa and lambda light chains can be used to determine disease status by immunohistochemistry or ELISA based assays.

Long Name

Immunoglobulin Lambda Light Chain

Alternate Names

Bence Jones protein; BJP; Constant region of lambda light chains; HCAK 1; HCAK1; Ig kappa chain C region; Ig lambda chain C regions; Ig Lambda Light Chain; IGKC; IGLC 1; IGLC 2; IGLC 3; IGLC; IGLC1; IGLC2; IGLC3; Immunoglobulin kappa constant region; Immunoglobulin kappa constant; Immunoglobulin kappa light chain; Immunoglobulin lambda constant 1; Kappa 1 immunoglobulin light chain; Km; Mcg marker; MGC111575; MGC62011; MGC72072; MGC88770; MGC88771; MGC88809; Paraprotein