Ig Lambda Light Chain Antibody (2496A)

R&D Systems | Catalog # MAB100492

R&D Systems
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Key Product Details

Species Reactivity

Human, Primate

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2496A
View all Ig Lambda Light Chain Primary Antibodies »

Product Specifications

Immunogen

Human Ig Lamda Light Chain

Specificity

Detects human Ig Lambda Light Chain in direct ELISAs. Detects human and primate Ig Lambda Light Chain in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ig Lambda Light Chain Antibody (2496A)

Detection of Human and Primate Ig Lambda Light Chain antibody by Western Blot.

Detection of Human and Primate Ig Lambda Light Chain by Western Blot.

Western blot shows lysates of human kappa light chain purified protein (negative sample), human lambda light chain purified protein, human serum, primate serum, mouse serum (negative sample), and rat serum (negative sample). PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Primate Ig Lambda Light Chain Monoclonal Antibody (Catalog # MAB100492) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Ig Lambda Light Chain at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Ig Lambda Light Chain Antibody (2496A)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human lambda light chain purified protein, Human serum, and Primate serum

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ig Lambda Light Chain

The immunoglobulin light chain is the smaller subunit of an antibody and in humans can be expressed in two types: as a kappa ( kappa ) or a lambda ( lambda ) chain.  Antibodies are produced by B-Cells that are clonal and each expresses only one type of light chain. The light chain class remains fixed for the life of the B lymphocyte. The ratio of kappa and lambda light chains can be used to determine disease status by immunohistochemistry or ELISA based assays.

Long Name

Immunoglobulin Lambda Light Chain

Alternate Names

Bence Jones protein, BJP, Constant region of lambda light chains, HCAK 1, HCAK1, Ig kappa chain C region, Ig lambda chain C regions, IGKC, IGLC, IGLC 1, IGLC 2, IGLC 3, IGLC1, IGLC2, IGLC3, Immunoglobulin kappa constant, Immunoglobulin kappa constant region, Immunoglobulin kappa light chain, Immunoglobulin lambda constant 1, Kappa 1 immunoglobulin light chain, Km, Mcg marker, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809, Paraprotein

Additional Ig Lambda Light Chain Products

Product Documents for Ig Lambda Light Chain Antibody (2496A)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Ig Lambda Light Chain Antibody (2496A)

For research use only

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