Key Product Details

Species Reactivity

Human, Primate

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1268B
View all IgG Primary Antibodies »

Product Specifications

Immunogen

Human IgG

Specificity

Detects human and primate IgG in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Primate IgG Antibody

Detection of Human and Primate IgG antibody by Western Blot.

Detection of Human and Primate IgG by Western Blot.

Western blot shows human serum, primate, mouse, and rat serum. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human/Primate IgG Monoclonal Antibody (Catalog # MAB1101) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for human and primate IgG at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human IgG antibody by Simple WesternTM.

Detection of Human IgG by Simple WesternTM.

Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for IgG at approximately 60 kDa (as indicated) using 2 µg/mL of Rabbit Anti-Human/Primate IgG Monoclonal Antibody (Catalog # MAB1101). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Primate IgG Antibody

Application
Recommended Usage

Simple Western

2 µg/mL
Sample: Human serum

Western Blot

0.05 µg/mL
Sample: Human serum and primate serum

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Human/Primate IgG Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Primate IgG Antibody

For research use only

Citations for Human/Primate IgG Antibody

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