Human/Primate IgG Antibody

Catalog # Availability Size / Price Qty
MAB1101-100
MAB1101-SP
Human/Primate IgG Antibody in Data
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Human/Primate IgG Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human and primate IgG in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1268B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.05 µg/mL
See below
Simple Western
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Primate IgG by Western Blot. View Larger

Detection of Human and Primate IgG by Western Blot. Western blot shows human serum, primate, mouse, and rat serum. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human/Primate IgG Monoclonal Antibody (Catalog # MAB1101) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for human and primate IgG at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human IgG by Simple Western<sup>TM</sup>. View Larger

Detection of Human IgG by Simple WesternTM. Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for IgG at approximately 60 kDa (as indicated) using 2 µg/mL of Rabbit Anti-Human/Primate IgG Monoclonal Antibody (Catalog # MAB1101). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG

Product Datasheets

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