Detection of Human PRKD3/nPKC nu by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human PRKD3/nPKC nu Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6478) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PRKD3/nPKC nu at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PRKD3/nPKC nu in MCF‑7 Human Cell Line.
PRKD3/nPKC nu was detected in immersion fixed MCF‑7 human breast cancer cell line using Sheep Anti-Human PRKD3/nPKC nu Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6478) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PRKD3/nPKC nu
PKC nu (Protein kinase C‑nu; also PKD3 and PRKD3) is a 105‑110 kDa member of the PKD subfamily, CAMK Ser/Thr protein kinase family of enzymes. It is expressed in highly diverse cell types such as pancreatic aciner cells, skeletal muscle cells, B cells and sensory neurons, and it is activated by PKC‑mediated phosphorylation. Active PKC nu associates with membrane compartments, and appears to participate with VAMP2 in cargo transport and vesicular trafficking. It also acts on class II HDACs in the nucleus, resulting in gene transcription. Human PKC nu is 890 amino acids (aa) in length. It contains two DAG‑type zinc finger regions (aa 154‑204 and 271‑321), one PH domain (aa 416‑532) and a large protein kinase domain (aa 576‑832). There are at least 25 utilized Ser/Thr phosphorylation sites that span the length of the molecule. PKC nu possesses one potential splice variant that shows a 17 aa substitution for aa 595‑890. Over aa 465‑559, human PKC nu is 92% aa identical to mouse PKC nu.
Protein Kinase D3
Entrez Gene IDs:
23683 (Human); 75292 (Mouse); 313834 (Rat)
EC 2.7.11; EC 22.214.171.124; EPK2; EPK2PKD3; nPKC nu; nPKC-nu; PKC nu; PKC-NU; PRKCN; PRKCNnPKC-NU; PRKD3; Protein kinase C nu type; protein kinase C, nu; protein kinase D3; Protein kinase EPK2; protein-serine/threonine kinase; serine/threonine-protein kinase D3
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