Human Progranulin/PGRN Antibody

Catalog # Availability Size / Price Qty
AF2420
AF2420-SP
Detection of Human Progranulin/PGRN by Western Blot.
3 Images
Product Details
Citations (16)
FAQs
Supplemental Products
Reviews (3)

Human Progranulin/PGRN Antibody Summary

Species Reactivity
Human
Specificity
Detects human Progranulin/PGRN in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Progranulin/PGRN
Thr18-Leu593
Accession # P28799
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Knockout Validated
Progranulin/PGRN is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in Progranulin/PGRN knockout HEK293T cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Progranulin/PGRN antibody by Western Blot. View Larger

Detection of Human Progranulin/PGRN by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2420) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for Progranulin/PGRN at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Progranulin antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Progranulin in Human Breast Cancer Tissue. Progranulin was detected in paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human Progranulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2420) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Knockout Validated Western Blot Shows Human Progranulin/PGRN Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human Progranulin/PGRN Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Progranulin knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2420) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Progranulin/PGRN at approximately 80 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Progranulin/PGRN

Progranulin, also known as acrogranin, PC cell-derived growth factor (PCDGF) and epithelin/granulin precursor, is a ubiquitously expressed, 88 kDa, secreted glycoprotein (1‑3). Structurally, it does not belong to any of the well-established growth factor families (4). Human Progranulin is 593 amino acids (aa) in length and contains a 17 aa signal sequence and 5 potential sites for N-linked glycosylation (Swiss Prot # P28799). It has a highly repetitive organization, containing seven tandem copies of a 55-57 aa consensus motif that contains 12 conserved cysteine residues: VXCX5-6CX5CCX8CCX6CCXDX2HCCPX4CX5-6CX2 (1). There is one alternate splice form for human Progranulin. This has a deletion of aa corresponding to aa 377-531 of the standard form. Progranulin is secreted as a full length form (2, 4), and may undergo proteolysis leading to the release of numerous peptides made from the seven tandem repeats, called the granulins (5-7). Human Progranulin shares 75% aa sequence identity with mouse and rat Progranulin. Progranulin is involved in the regulation of cellular proliferation, as well as differentiation, development, and pathological processes (4). It has been isolated as a differentially expressed gene during mesothelial differentiation (8), macrophage development (9), the development of rheumatoid arthritis and osteoarthritis (10), sexual differentiation of the brain (11), and has also been shown to be a mediator of cartilage proliferation and of wound response and tissue repair (4, 12 13). High levels of Progranulin expression have been found to be associated with several human cancers and are believed to contribute to tumorigenesis in breast cancer, clear cell renal carcinoma, invasive ovarian carcinoma, glioblastoma, adipocyte teratoma, and multiple myeloma (4-5, 12, 14-19). In addition, mutations in the Progranulin gene are a cause of frontotemporal dementia, and increased expression of Progranulin is seen in activated microglia in many neurodegenerative diseases including Creutzfeldt-Jakob disease, motor neuron disease and Alzheimer's disease (20). Mutations in Progranulin causing neurodegenerative disease indicate that Progranulin is important for neuronal survival (20).

References
  1. Plowman, G.D. et al. (1992) J. Biol. Chem. 267:13073.
  2. Zhou, J. et al. (1993) J. Biol. Chem. 268:10863.
  3. Liu, Y. et al. (2007) BMC Cancer 7:22.
  4. Xu, K. et al. (2007) J. Biol. Chem. 282:11347.
  5. Davidson, B. et al. (2004) Cancer 100:2139.
  6. Zanocco-Marani, T. et al. (1999) Cancer Res. 59:5331.
  7. Lu, R. and G. Serrero (2000) Proc. Natl. Acad. Sci. USA 97:3993.
  8. Sun, X. et al. (2004) Am. J. Respir. Cell Mol. Biol. 30:510.
  9. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:727.
  10. Justen, H.P. et al. (2000) Mol. Cell Biol. Res. Commun. 3:165.
  11. Suzuki, M. and M. Nishiahara (2002) Mol. Genet. Metab. 75:31.
  12. He, Z. et al. (2003) Nat. Med. 9:225.
  13. Zhu, J. et al. (2002) Cell 111:867.
  14. He, Z. and A. Bateman (2003) J. Mol. Med. 81:600.
  15. Bateman, A. et al. (1990) Biochem. Biophys. Res. Commun. 173:1161.
  16. Gonzalez, E.M. et al. (2003) J. Biol. Chem. 278:38113.
  17. Jones, M.B. et al. (2003) Gynecol. Oncol. 88:S136.
  18. Wang, W. et al. (2003) Clin. Cancer Res. 9:2221.
  19. Zhang, H. and G. Serrero (1998) Proc. Natl. Acad. Sci. USA 95:14202.
  20. Baker, M. et al. (2006) Nature 442:916.
Entrez Gene IDs
2896 (Human); 14824 (Mouse)
Alternate Names
Acrogranin; CLN11; GEP; GP88; granulin precursor; GRN; PCDGF; PEPI; PGRN; Proepithelin; Progranulin

Product Datasheets

You must select a language.

x

Citations for Human Progranulin/PGRN Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Tumor co-expression of progranulin and sortilin as a prognostic biomarker in breast cancer
    Authors: K Berger, S Rhost, S Rafnsdótti, É Hughes, Y Magnusson, M Ekholm, O Stål, L Rydén, G Landberg
    BMC Cancer, 2021;21(1):185.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice
    Authors: J Frew, HB Nygaard
    Acta neuropathologica communications, 2021;9(1):57.
    Species: Mosue
    Sample Types: Whole Tissue
    Applications: IHC
  3. Regulated control of gene therapies by drug-induced splicing
    Authors: AM Monteys, AA Hundley, PT Ranum, L Tecedor, A Muehlmatt, E Lim, D Lukashev, R Sivasankar, BL Davidson
    Nature, 2021;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: FRET
  4. Delivering progranulin to neuronal lysosomes protects against excitotoxicity
    Authors: SE Davis, JR Roth, Q Aljabi, AR Hakim, KE Savell, JJ Day, AE Arrant
    The Journal of Biological Chemistry, 2021;0(0):100993.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Premature termination codon readthrough upregulates progranulin expression and improves lysosomal function in preclinical models of GRN deficiency
    Authors: J Frew, A Baradaran-, AD Balgi, X Wu, TD Yan, S Arns, FS Shidmoossa, J Tan, JB Jaquith, KR Jansen-Wes, FC Lynn, FB Gao, L Petrucelli, HH Feldman, IR Mackenzie, M Roberge, HB Nygaard
    Mol Neurodegener, 2020;15(1):21.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Characterization of lysosomal proteins Progranulin and Prosaposin and their interactions in Alzheimer's disease and aged brains: increased levels correlate with neuropathology
    Authors: A Mendsaikha, I Tooyama, JP Bellier, GE Serrano, LI Sue, LF Lue, TG Beach, DG Walker
    Acta Neuropathol Commun, 2019;7(1):215.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  7. Loss of Tmem106b is unable to ameliorate frontotemporal dementia-like phenotypes in an AAV mouse model of C9ORF72-repeat induced toxicity
    Authors: AM Nicholson, X Zhou, RB Perkerson, TM Parsons, J Chew, M Brooks, M DeJesus-He, NA Finch, BJ Matchett, A Kurti, KR Jansen-Wes, E Perkerson, L Daughrity, M Castanedes, L Rousseau, V Phillips, F Hu, TF Gendron, ME Murray, DW Dickson, JD Fryer, L Petrucelli, R Rademakers
    Acta Neuropathol Commun, 2018;6(1):42.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. The lysosomal protein cathepsin L is a progranulin protease
    Authors: CW Lee, JN Stankowski, J Chew, CN Cook, YW Lam, S Almeida, Y Carlomagno, KF Lau, M Prudencio, FB Gao, M Bogyo, DW Dickson, L Petrucelli
    Mol Neurodegener, 2017;12(1):55.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Lysosomal processing of progranulin
    Authors: X Zhou, DH Paushter, T Feng, L Sun, T Reinheckel, F Hu
    Mol Neurodegener, 2017;12(1):62.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Evidence of the innate antiviral and neuroprotective properties of progranulin.
    Authors: Suh H, Lo Y, Choi N, Letendre S, Lee S
    PLoS ONE, 2014;9(5):e98184.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Regulation of progranulin expression in human microglia and proteolysis of progranulin by matrix metalloproteinase-12 (MMP-12).
    Authors: Suh HS, Choi N, Tarassishin L, Lee SC
    PLoS ONE, 2012;7(4):e35115.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Pathogenic cysteine mutations affect progranulin function and production of mature granulins.
    Authors: Wang J, Van Damme P, Cruchaga C, Gitcho MA, Vidal JM, Seijo-Martinez M, Wang L, Wu JY, Robberecht W, Goate A
    J. Neurochem., 2010;112(5):1305-15.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Progranulin modulates zebrafish motoneuron development in vivo and rescues truncation defects associated with knockdown of Survival motor neuron 1.
    Authors: Chitramuthu BP, Baranowski DC, Kay DG
    Mol Neurodegener, 2010;5(0):41.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. Progranulin expression correlates with dense-core amyloid plaque burden in Alzheimer disease mouse models.
    Authors: Pereson S, Wils H, Kleinberger G, McGowan E, Vandewoestyne M, Van Broeck B, Joris G, Cuijt I, Deforce D, Hutton M, Van Broeckhoven C, Kumar-Singh S
    J. Pathol., 2009;219(2):173-81.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. HDDD2 is a familial frontotemporal lobar degeneration with ubiquitin-positive, tau-negative inclusions caused by a missense mutation in the signal peptide of progranulin.
    Authors: Mukherjee O, Pastor P, Cairns NJ, Chakraverty S, Kauwe JS, Shears S, Behrens MI, Budde J, Hinrichs AL, Norton J, Levitch D, Taylor-Reinwald L, Gitcho M, Tu PH, Tenenholz Grinberg L, Liscic RM, Armendariz J, Morris JC, Goate AM
    Ann. Neurol., 2006;60(3):314-22.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  16. Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins.
    Authors: Cherepanova N, Shrimal S, Gilmore R
    J Cell Biol, 0;206(4):525-39.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Progranulin/PGRN Antibody

Average Rating: 4 (Based on 3 Reviews)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human Progranulin/PGRN Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human Progranulin/PGRN Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Progranulin/PGRN Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Progranulin/PGRN Antibody
By Carly Garrison on 10/31/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human