Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Protocadherin alpha 1 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Protocadherin alpha 1
Gln30-Pro689
Accession # Q9Y5I3

Specificity

Detects human Protocadherin alpha 1 (PCDH alpha 1) in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Protocadherin  alpha 1 Antibody

Detection of Human Protocadherin a1 antibody by Western Blot.

Detection of Human Protocadherin alpha 1 by Western Blot.

Western blot shows lysates of human brain cortex tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Protocadherin a1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5064) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Protocadherin a1 at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Protocadherin a1 antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

Protocadherin alpha 1 in Mouse Brain.

Protocadherin a1 was detected in immersion fixed frozen sections of mouse brain (cerebellum) using 10 µg/mL Sheep Anti-Human Protocadherin a1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5064) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counter-stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Protocadherin a1 antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

Protocadherin alpha 1 in Mouse Brain.

Protocadherin a1 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Human Protocadherin a1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5064) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counter-stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Human Protocadherin  alpha 1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse brain (cerebellum)

Western Blot

1 µg/mL
Sample: Human brain cortex tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Protocadherin alpha 1

Protocadherin alpha 1 (PCDH alpha 1) is a group A member of the Protocadherin family of molecules. It is expressed in synaptic membranes of neurons, forms obligate Ca++ independent complexes with various PCDH  gamma family members and demonstrates heterophilic binding activity towards beta 1 integrins. Human PCDH alpha 1 is 921 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 668 aa extracellular domain (ECD) plus a 232 aa cytoplasmic region. Both regions contribute to PCDH-gamma binding. There are six cadherin domains in the ECD. ADAM10 cleavage generates an 80‑90 kDa soluble fragment and a soluble splice form exists that is missing aa 535‑798. Over aa 30‑689, human PCDH alpha 1 shares 83% aa identity with mouse PCDH alpha 1.

Alternate Names

PCDHA1

Entrez Gene IDs

56147 (Human); 116731 (Mouse); 393085 (Rat)

Gene Symbol

PCDHA1

UniProt

Q9Y5I3

Additional Protocadherin alpha 1 Products

Product Documents for Human Protocadherin  alpha 1 Antibody

Certificate of Analysis

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Product Specific Notices for Human Protocadherin  alpha 1 Antibody

For research use only

Related Research Areas

Cadherin Superfamily

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Protocols

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