Human Protocadherin alpha 1 Antibody
Human Protocadherin alpha 1 Antibody Summary
Gln30-Pro689
Accession # Q9Y5I3
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Protocadherin alpha 1 by Western Blot. Western blot shows lysates of human brain cortex tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Protocadherin a1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5064) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Protocadherin a1 at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Protocadherin alpha 1 in Mouse Brain. Protocadherin a1 was detected in immersion fixed frozen sections of mouse brain (cerebellum) using 10 µg/mL Sheep Anti-Human Protocadherin a1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5064) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counter-stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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Protocadherin alpha 1 in Mouse Brain. Protocadherin a1 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Human Protocadherin a1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5064) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counter-stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Protocadherin alpha 1
Protocadherin alpha 1 (PCDH alpha 1) is a group A member of the Protocadherin family of molecules. It is expressed in synaptic membranes of neurons, forms obligate Ca++ independent complexes with various PCDH gamma family members and demonstrates heterophilic binding activity towards beta 1 integrins. Human PCDH alpha 1 is 921 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 668 aa extracellular domain (ECD) plus a 232 aa cytoplasmic region. Both regions contribute to PCDH-gamma binding. There are six cadherin domains in the ECD. ADAM10 cleavage generates an 80‑90 kDa soluble fragment and a soluble splice form exists that is missing aa 535‑798. Over aa 30‑689, human PCDH alpha 1 shares 83% aa identity with mouse PCDH alpha 1.
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