Human Protocadherin  alpha 1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Protocadherin alpha 1 (PCDH alpha 1) in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Protocadherin alpha 1
    Gln30-Pro689
    Accession # Q9Y5I3
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Protocadherin  alpha 1 in Mouse Brain. Protocadherin  alpha 1 was detected in immersion fixed frozen sections of mouse brain (cerebellum) using 10 µg/mL Sheep Anti-Human Protocadherin  alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5064) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counter­stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Human Protocadherin  alpha 1 by Western Blot. Western blot shows lysates of human brain cortex tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Protocadherin  alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5064) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Protocadherin  alpha 1 at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunohistochemistry
Protocadherin  alpha 1 in Mouse Brain. Protocadherin  alpha 1 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Human Protocadherin  alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5064) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counter­stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Protocadherin alpha 1

Protocadherin alpha 1 (PCDH alpha 1) is a group A member of the Protocadherin family of molecules. It is expressed in synaptic membranes of neurons, forms obligate Ca++ independent complexes with various PCDH  gamma family members and demonstrates heterophilic binding activity towards beta 1 integrins. Human PCDH alpha 1 is 921 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 668 aa extracellular domain (ECD) plus a 232 aa cytoplasmic region. Both regions contribute to PCDH-gamma binding. There are six cadherin domains in the ECD. ADAM10 cleavage generates an 80‑90 kDa soluble fragment and a soluble splice form exists that is missing aa 535‑798. Over aa 30‑689, human PCDH alpha 1 shares 83% aa identity with mouse PCDH alpha 1.

  • Entrez Gene IDs:
    56147 (Human); 116731 (Mouse); 393085 (Rat)
  • Alternate Names:
    KIAA0345-like 13; PCDHA1; PCDH-ALPHA1; PCDH-alpha-1; Protocadherin alpha 1; protocadherin alpha-1
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