Key Product Details

Species Reactivity

Human

Applications

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 814403
View all Protogenin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Protogenin
Phe36-Ala952
Accession # Q2VWP7

Specificity

Detects human Protogenin in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human Protogenin Antibody

Adhesion Induced by Protogenin and Neutralization by Human Protogenin Antibody.

Adhesion Induced by Protogenin and Neutralization by Human Protogenin Antibody.

Recombinant Human Protogenin (Catalog # 6795-PR) supports the adhesion in the P19 mouse embryonal carcinoma cell line in a dose-dependent manner (orange line), as measured by Calcein AM. Adhesion elicited by Recombinant Human Protogenin (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Protogenin Monoclonal Antibody (Catalog # MAB6795). The ND50 is typically 0.4-2.4 µg/mL.

Applications for Human Protogenin Antibody

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize Protogenin-induced adhesion in the P19 mouse embryonal carcinoma cell line. The Neutralization Dose (ND50) is typically 0.4-2.4 µg/mL in the presence of 10 µg/mL Recombinant Human Protogenin.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Protogenin

PRTG (Protogenin; also Protein Shen-Dan) is a 175-180 kDa transmembrane (TM) member of the DEAL (DCC et al) family of proteins. It is expressed in the embryo in the period before E10.5 in mouse (day 28 in human), and appears in a widespread distribution. Through homophilic intercellular interaction, it regulates initial epiblast cell migration/ingression, thus impacting somite organization. Via its actions as a neural tube epithelial cell receptor, it binds soluble 60 kDa ERdj3, resulting in a suppression of neuron differentiation. Mature human PRTG is an 1115 amino acid (aa) type I TM glycoprotein. It contains a 917 aa extracellular domain (ECD)
(aa 36-952) and a 180 aa cytoplasmic region. The ECD possesses four consecutive Ig-like domains (aa 36-411) followed by five FN type III domains (aa 419-914). There is one potential splice variant that shows a 49 aa substitution for Ala325 coupled to a deletion of aa 516-541. The ECD of human PRTG shares 93% aa identity with the ECD of mouse PRTG.

Alternate Names

IGDCC5, PRTG, RPTG, Shen-Dan

Entrez Gene IDs

283659 (Human); 235472 (Mouse); 315806 (Rat)

Gene Symbol

PRTG

UniProt

Q2VWP7 (Human)

Additional Protogenin Products

Product Documents for Human Protogenin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Protogenin Antibody

For research use only

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