Human Protogenin Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Protogenin in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 814403
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Protogenin
    Phe36-Ala952
    Accession # Q2VWP7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize Protogenin-induced adhesion in the P19 mouse embryonal carcinoma cell line. The Neutralization Dose (ND50) is typically 0.4-2.4 µg/mL in the presence of 10 µg/mL Recombinant Human Protogenin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Adhesion Induced by Protogenin and Neutralization by Human Protogenin Antibody. Recombinant Human Protogenin (Catalog # 6795-PR) supports the adhesion in the P19 mouse embryonal carcinoma cell line in a dose-dependent manner (orange line), as measured by Calcein AM. Adhesion elicited by Recombinant Human Protogenin (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Protogenin Monoclonal Antibody (Catalog # MAB6795). The ND50 is typically
0.4-2.4 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Protogenin
PRTG (Protogenin; also Protein Shen-Dan) is a 175-180 kDa transmembrane (TM) member of the DEAL (DCC et al) family of proteins. It is expressed in the embryo in the period before E10.5 in mouse (day 28 in human), and appears in a widespread distribution. Through homophilic intercellular interaction, it regulates initial epiblast cell migration/ingression, thus impacting somite organization. Via its actions as a neural tube epithelial cell receptor, it binds soluble 60 kDa ERdj3, resulting in a suppression of neuron differentiation. Mature human PRTG is an 1115 amino acid (aa) type I TM glycoprotein. It contains a 917 aa extracellular domain (ECD)
(aa 36-952) and a 180 aa cytoplasmic region. The ECD possesses four consecutive Ig-like domains (aa 36-411) followed by five FN type III domains (aa 419-914). There is one potential splice variant that shows a 49 aa substitution for Ala325 coupled to a deletion of aa 516-541. The ECD of human PRTG shares 93% aa identity with the ECD of mouse PRTG.
  • Entrez Gene IDs:
    283659 (Human); 235472 (Mouse); 315806 (Rat)
  • Alternate Names:
    FLJ12795; FLJ13221; FLJ25756; IGDCC5; immunoglobulin superfamily, DCC subclass, member 5; Protein Shen-Dan; protogenin homolog (Gallus gallus); Protogenin; PRTG; RPTG; Shen-Dan
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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