Human PTH1R/PTHR1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human PTH1 R by Western Blot.
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Product Details
Citations (1)
Supplemental Products

Human PTH1R/PTHR1 Antibody Summary

Species Reactivity
Detects human PTH1 R in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
Chinese hamster ovary cell line CHO-derived recombinant human PTH1 R
Accession # Q03431
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PTH1 R antibody by Western Blot. View Larger

Detection of Human PTH1 R by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Human PTH1 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5709) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PTH1 R at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry PTH1 R antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

PTH1 R in Human Kidney. PTH1 R was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human PTH1 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5709) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTH1R/PTHR1

PTH1 R (Parathyroid hormone receptor # 1; also PTH/PTHr receptor) is a predicted 66 - 68 kDa member of the G-protein coupled receptor family # 2. It is expressed on a variety of cell types, including hepatocytes, renal epithelium, smooth muscle cells, and osteoblasts plus chondrocytes. PTH1 R is a receptor for both PTH and PTHrP, and PTH binding promotes Ca++-release from bone, mediated by osteoclast formation. Mature human PTH1 R is a 7-transmembrane glycoprotein 567 amino acids (aa) in length. It contains a long, ligand-binding N-terminal extracellular region (aa 27 - 188) and a 130 aa cytoplasmic C-terminal domain. Single aa changes such as Gly121Glu, Ala122Thr and Arg255His can impair PTH1 R signaling. Over aa 1 - 189, human PTH1 R shares 88% aa identity with mouse PTH1 R.

Long Name
Parathyroid Hormone Receptor 1
Entrez Gene IDs
5745 (Human); 19228 (Mouse); 56813 (Rat)
Alternate Names
MGC138426; parathyroid hormone 1 receptorMGC138452; parathyroid hormone/parathyroid hormone-related peptide receptor; parathyroid hormone/parathyroid hormone-related protein receptor; PFE; PTH/PTHr receptor; PTH/PTHrP type I receptor; PTH1 receptor; PTH1R; PTHR; PTHR1; PTHRPTHR1parathyroid hormone receptor 1; seven transmembrane helix receptor

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Citation for Human PTH1R/PTHR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Single cell transcriptional and chromatin accessibility profiling redefine cellular heterogeneity in the adult human kidney
    Authors: Y Muto, PC Wilson, N Ledru, H Wu, H Dimke, SS Waikar, BD Humphreys
    Nature Communications, 2021;12(1):2190.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC


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