Human R-Spondin 3 DuoSet ELISA

R&D Systems | Catalog # DY3500

R&D Systems
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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

15.6-1000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human R-Spondin 3 DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all R-Spondin 3 ELISA Kits »

Product Summary for Human R-Spondin 3 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human R-Spondin 3. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human R-Spondin 3 DuoSet ELISA

Human R-Spondin 3 ELISA Standard Curve

Human R-Spondin 3 ELISA Standard Curve

Kit Contents for Human R-Spondin 3 DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)

Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)

Microplates: (Catalog # DY990), or equivalent

Plate Sealers: (Catalog # DY992), or equivalent

*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: R-Spondin 3

R-Spondin 3 (RSPO3; Cristin 1) is a secreted Wnt regulating protein that is critical for development of the placental labyrinthine layer. It is expressed in the roof plate, tail, somites, otic vesicles, cephalic mesoderm, truncus arteriosus, atrioventricular canal of the developing heart, and strongly but transiently in developing limbs. R-Spondins regulate Wnt/β-catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK-1-mediated internalization. Reports differ on whether R-Spondins bind LRP-6 directly.

Long Name

Roof Plate-specific Spondin 3

Alternate Names

Cristin 1, PWTSR, RSPO3, RSpondin 3, THSD2

Entrez Gene IDs

84870 (Human); 72780 (Mouse); 102132009 (Cynomolgus Monkey)

Gene Symbol

RSPO3

Additional R-Spondin 3 Products

Product Documents for Human R-Spondin 3 DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human R-Spondin 3 DuoSet ELISA

For research use only

Related Research Areas

Wnt Signaling Modulators

Citations for Human R-Spondin 3 DuoSet ELISA

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  • Human R-Spondin 3 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 05/16/2022
    We used this kit to quantify R-Spondin 3 in human serum and plasma, works well at lower dilution.
    Human R-Spondin 3 DuoSet ELISA DY3500

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