Human/Rat alpha -Fetoprotein/AFP Antibody

(1 Review)
  
  • Species Reactivity
    Human, Rat
  • Specificity
    Detects human alpha -Fetoprotein in direct ELISAs and Western blots.
  • Source
    Polyclonal Chicken IgY
  • Purification
    Antigen Affinity-purified from egg yolks
  • Immunogen
    Human umbilical cord serum-derived alpha -Fetoprotein
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Simple Western
    2 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Rat alpha ‑Fetoprotein/AFP by Western Blot. Western blot shows lysates of human liver tissue and H4‑II‑E‑C3 rat hepatoma cell line. PVDF membrane was probed with 0.2 µg/mL of Chicken Anti-Human/Rat alpha ‑Fetoprotein/
AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
alpha ‑Fetoprotein/AFP in BG01V Human Embryonic Stem Cells. alpha ‑Fetoprotein/AFP was detected in immersion fixed BG01V human embryonic stem cells using Chicken Anti-Human/Rat alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Chicken IgY Secondary Antibody (red; Catalog # NL016) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
alpha ‑Fetoprotein/AFP in Human Liver cancer alpha ‑Fetoprotein/AFP was detected in immersion fixed paraffin-embedded sections of human liver cancer using 15 µg/mL Chicken anti-Human/
Rat alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human alpha ‑Fetoprotein/AFP by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) using 2 µg/mL of Chicken Anti-Human/Rat alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Fetoprotein/AFP

alpha ‑Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors.

  • Entrez Gene IDs:
    174 (Human); 11576 (Mouse); 24177 (Rat)
  • Alternate Names:
    AFP; Alpha-1-fetoprotein; alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP
Related Research Areas
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Mounting Medium

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Antigen Retrieval Reagent Sampler (50 mL each)

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Isotype Controls
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Normal Chicken IgY Control

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Secondary Antibodies
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Goat Anti-Chicken IgY Antibody

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Goat Anti-Chicken IgY NorthernLights™ NL493-conjugated Antibody

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Goat Anti-Chicken IgY Biotinylated Antibody

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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human/Rat  alpha -Fetoprotein/AFP Antibody AF1369
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 WB Human Anonymous 06/13/2017
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Western Blot Human/Rat  alpha -Fetoprotein/AFP Antibody AF1369
Western Blot: Human/Rat alpha -Fetoprotein/AFP Antibody [AF1369]

Summary

ApplicationWestern Blot
Sample TestedPlasma
SpeciesHuman

Other Experimental Details

Other Experimental DetailsPlasma sample 10 µg / wellAntigen: alpha -Fetoprotein (AFP)Primary antibody: Anti-Human AFP antibody (Polyclonal Chicken IgY) from R&D systems; Cat No. AF1369Secondary antibody_1: Goat anti-chicken IgY-unconjugated (Dilution: 1:5000) from R&D systems; Cat No. AF010Secondary antibody_2: Donkey anti-goat IgG-HRP from Santa Cruz; Cat no. sc-2020) Molecular weight of the antigen: 68 kDaPercentage of SDS-PAGE gel: 12.5 %Run time: 3.5 hoursWet transfer @ 100 V for 1 hr

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