Human/Rat alpha-Fetoprotein/AFP Antibody

Catalog # Availability Size / Price Qty
AF1369
AF1369-SP
Detection  of Human and Rat  alpha ‑Fetoprotein/AFP by Western Blot.
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Citations (1)
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Reviews (1)

Human/Rat alpha-Fetoprotein/AFP Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human alpha -Fetoprotein in direct ELISAs and Western blots.
Source
Polyclonal Chicken IgY
Purification
Antigen Affinity-purified from egg yolks
Immunogen
Human umbilical cord serum-derived alpha -Fetoprotein
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below
Knockout Validated
alpha ‑Fetoprotein is specifically detected in HepG2 human hepatocellular carcinoma cell line but is not detectable in alpha ‑Fetoprotein knockout HepG2 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human and Rat  alpha ‑Fetoprotein/AFP by Western Blot. View Larger

Detection of Human and Rat alpha ‑Fetoprotein/AFP by Western Blot. Western blot shows lysates of human liver tissue and H4‑II‑E‑C3 rat hepatoma cell line. PVDF membrane was probed with 0.2 µg/mL of Chicken Anti-Human/Rat alpha ‑Fetoprotein/
AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry alpha ‑Fetoprotein/AFP in BG01V Human Embryonic Stem Cells. View Larger

alpha ‑Fetoprotein/AFP in BG01V Human Embryonic Stem Cells. alpha ‑Fetoprotein/AFP was detected in immersion fixed BG01V human embryonic stem cells using Chicken Anti-Human/Rat alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Chicken IgY Secondary Antibody (red; Catalog # NL016) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry alpha ‑Fetoprotein/AFP in Human Liver cancer View Larger

alpha ‑Fetoprotein/AFP in Human Liver cancer alpha ‑Fetoprotein/AFP was detected in immersion fixed paraffin-embedded sections of human liver cancer using 15 µg/mL Chicken anti-Human/
Rat alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection  of Human  alpha ‑Fetoprotein/AFP by Simple  WesternTM. View Larger

Detection of Human alpha ‑Fetoprotein/AFP by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) using 2 µg/mL of Chicken Anti-Human/Rat alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated alpha ‑Fetoprotein/AFP  Specificity is Shown by Immunocytochemistry in Knockout Cell Line. View Larger

alpha ‑Fetoprotein/AFP Specificity is Shown by Immunocytochemistry in Knockout Cell Line. alpha ‑Fetoprotein/AFP was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line but is not detected in alpha ‑Fetoprotein/AFP knockout (KO) HepG2 Human Cell Line cell line using Chicken Anti-Human/Rat alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Chicken IgY Secondary Antibody (green; Catalog # NL018) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Fetoprotein/AFP

alpha ‑Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors.

Entrez Gene IDs
174 (Human); 11576 (Mouse); 24177 (Rat)
Alternate Names
AFP; Alpha-1-fetoprotein; alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP

Product Datasheets

Citation for Human/Rat alpha-Fetoprotein/AFP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A Marfan syndrome human induced pluripotent stem cell line with a heterozygous FBN1 c.4082G>A mutation, ISMMSi002-B, for disease modeling
    Authors: S Klein, JL Dvornik, AR Yarrabothu, C Schaniel
    Stem Cell Res, 2017;23(0):73-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

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Reviews for Human/Rat alpha-Fetoprotein/AFP Antibody

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Human/Rat alpha -Fetoprotein/AFP Antibody
By Anonymous on 06/13/2017
Application: WB Sample Tested: Plasma Species: Human

Plasma sample 10 µg / well

Antigen: α-Fetoprotein (AFP)

Primary antibody: Anti-Human AFP antibody (Polyclonal Chicken IgY) from R&D systems; Cat No. AF1369

Secondary antibody_1: Goat anti-chicken IgY-unconjugated (Dilution: 1:5000) from R&D systems; Cat No. AF010

Secondary antibody_2: Donkey anti-goat IgG-HRP from Santa Cruz; Cat no. sc-2020)

Molecular weight of the antigen: 68 kDa

Percentage of SDS-PAGE gel: 12.5 %

Run time: 3.5 hours

Wet transfer @ 100 V for 1 hr