Human/Rat alpha-Fetoprotein/AFP Antibody

R&D Systems | Catalog # AF1369

R&D Systems

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Knockout Validated, Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Western Blot, Immunocytochemistry, ELISA Capture

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY
View all alpha-Fetoprotein/AFP Primary Antibodies »

Product Specifications

Immunogen

Human umbilical cord serum-derived alpha -Fetoprotein

Specificity

Detects human alpha -Fetoprotein in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Scientific Data Images for Human/Rat alpha-Fetoprotein/AFP Antibody

Detection of Human and Rat a-Fetoprotein/AFP antibody by Western Blot.

Detection of Human and Rat alpha ‑Fetoprotein/AFP by Western Blot.

Western blot shows lysates of human liver tissue and H4‑II‑E‑C3 rat hepatoma cell line. PVDF membrane was probed with 0.2 µg/mL of Chicken Anti-Human/Rat alpha ‑Fetoprotein/ AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
a-Fetoprotein/AFP antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

alpha ‑Fetoprotein/AFP in BG01V Human Embryonic Stem Cells.

a-Fetoprotein/AFP was detected in immersion fixed BG01V human embryonic stem cells using Chicken Anti-Human/Rat a-Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Chicken IgY Secondary Antibody (red; Catalog # NL016) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
a-Fetoprotein/AFP antibody in Human Liver cancer

alpha ‑Fetoprotein/AFP in Human Liver cancer

alpha ‑Fetoprotein/AFP was detected in immersion fixed paraffin-embedded sections of human liver cancer using 15 µg/mL Chicken anti-Human/ Rat alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human a-Fetoprotein/AFP antibody by Simple WesternTM.

Detection of Human alpha ‑Fetoprotein/AFP by Simple WesternTM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) using 2 µg/mL of Chicken Anti-Human/Rat alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
a-Fetoprotein/AFP Antibody Specificity is Shown by Immunocytochemistry antibody in Knockout Cell Line.

alpha ‑Fetoprotein/AFP Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

a-Fetoprotein/AFP was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line but is not detected in a-Fetoprotein/AFP knockout (KO) HepG2 Human Cell Line cell line using Chicken Anti-Human/Rat a-Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Chicken IgY Secondary Antibody (green; Catalog # NL018) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Rat alpha-Fetoprotein/AFP Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver cancer

Knockout Validated

alpha ‑Fetoprotein is specifically detected in HepG2 human hepatocellular carcinoma cell line but is not detectable in alpha ‑Fetoprotein knockout HepG2 cell line.

Simple Western

2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Western Blot

0.2 µg/mL
Sample: Human liver tissue and H4‑II‑E‑C3 rat hepatoma cell line

Reviewed Applications

Read 2 reviews rated 5 using AF1369 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from egg yolks

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alpha-Fetoprotein/AFP

alpha ‑Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors.

Alternate Names

AFP, DSCAM2

Entrez Gene IDs

174 (Human); 11576 (Mouse); 24177 (Rat)

Gene Symbol

AFP

Additional alpha-Fetoprotein/AFP Products

Product Documents for Human/Rat alpha-Fetoprotein/AFP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Rat alpha-Fetoprotein/AFP Antibody

For research use only

Citations for Human/Rat alpha-Fetoprotein/AFP Antibody

Customer Reviews for Human/Rat alpha-Fetoprotein/AFP Antibody (2)

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2 Customer Ratings
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  • Human/Rat alpha -Fetoprotein/AFP Antibody
    Name: David Ivancic
    Application: Immunohistochemistry-Paraffin
    Sample Tested: human fetal liver
    Species: Human
    Verified Customer | Posted 03/26/2018
    Normal Human Fetal Liver-Paraffin Section
    Formalin/PFA-fixed paraffin-embedded sections Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer pH 6.0 Sea Block as blocking agent for 30 minutes Incubation is overnight at 4degC AFP antibody 1/100 in Sea Block Goat Anti-Chicken IgY H&L (Alexa Fluor® 555) preadsorbed secondary DAPI counterstain
    Human/Rat alpha-Fetoprotein/AFP Antibody AF1369
  • Human/Rat alpha -Fetoprotein/AFP Antibody
    Name: Jagadeesh Janjanam
    Application: Western Blot
    Sample Tested: Plasma
    Species: Human
    Verified Customer | Posted 06/13/2017
    Plasma sample 10 µg / well Antigen: alpha -Fetoprotein (AFP) Primary antibody: Anti-Human AFP antibody (Polyclonal Chicken IgY) from R&D systems; Cat No. AF1369 Secondary antibody_1: Goat anti-chicken IgY-unconjugated (Dilution: 1:5000) from R&D systems; Cat No. AF010 Secondary antibody_2: Donkey anti-goat IgG-HRP from Santa Cruz; Cat no. sc-2020) Molecular weight of the antigen: 68 kDa Percentage of SDS-PAGE gel: 12.5 % Run time: 3.5 hours Wet transfer @ 100 V for 1 hr
    Human/Rat alpha-Fetoprotein/AFP Antibody AF1369

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Protocols

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