Human/Rat alpha-Fetoprotein/AFP Antibody
Human/Rat alpha-Fetoprotein/AFP Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human and Rat alpha ‑Fetoprotein/AFP by Western Blot. Western blot shows lysates of human liver tissue and H4‑II‑E‑C3 rat hepatoma cell line. PVDF membrane was probed with 0.2 µg/mL of Chicken Anti-Human/Rat alpha ‑Fetoprotein/ AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
View Larger
alpha ‑Fetoprotein/AFP in BG01V Human Embryonic Stem Cells. a-Fetoprotein/AFP was detected in immersion fixed BG01V human embryonic stem cells using Chicken Anti-Human/Rat a-Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Chicken IgY Secondary Antibody (red; Catalog # NL016) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
View Larger
alpha ‑Fetoprotein/AFP in Human Liver cancer alpha ‑Fetoprotein/AFP was detected in immersion fixed paraffin-embedded sections of human liver cancer using 15 µg/mL Chicken anti-Human/ Rat alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
View Larger
Detection of Human alpha ‑Fetoprotein/AFP by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) using 2 µg/mL of Chicken Anti-Human/Rat alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
View Larger
alpha ‑Fetoprotein/AFP Specificity is Shown by Immunocytochemistry in Knockout Cell Line. a-Fetoprotein/AFP was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line but is not detected in a-Fetoprotein/AFP knockout (KO) HepG2 Human Cell Line cell line using Chicken Anti-Human/Rat a-Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Chicken IgY Secondary Antibody (green; Catalog # NL018) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Fetoprotein/AFP
alpha ‑Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors.
Product Datasheets
Citation for Human/Rat alpha-Fetoprotein/AFP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
A Marfan syndrome human induced pluripotent stem cell line with a heterozygous FBN1 c.4082G>A mutation, ISMMSi002-B, for disease modeling
Authors: S Klein, JL Dvornik, AR Yarrabothu, C Schaniel
Stem Cell Res, 2017;23(0):73-76.
Species: Human
Sample Types: Whole Cells
Applications: ICC
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for Human/Rat alpha-Fetoprotein/AFP Antibody
Average Rating: 5 (Based on 1 Review)
Have you used Human/Rat alpha-Fetoprotein/AFP Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
Plasma sample 10 µg / well
Antigen: α-Fetoprotein (AFP)
Primary antibody: Anti-Human AFP antibody (Polyclonal Chicken IgY) from R&D systems; Cat No. AF1369
Secondary antibody_1: Goat anti-chicken IgY-unconjugated (Dilution: 1:5000) from R&D systems; Cat No. AF010
Secondary antibody_2: Donkey anti-goat IgG-HRP from Santa Cruz; Cat no. sc-2020)
Molecular weight of the antigen: 68 kDa
Percentage of SDS-PAGE gel: 12.5 %
Run time: 3.5 hours
Wet transfer @ 100 V for 1 hr
