Detection of Human and Rat Neuroligin 1/ NLGN1 by Western Blot. Western blot shows lysates of rat embryonic cortical neurons, rat embryonic hippocampal neurons, and human brain (cortex) tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti‑Rat Neuroligin 1/ NLGN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4340) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neuroligin 1/NLGN1 at approximately 117 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Neuroligin 1/NLGN1 in Rat Brain. Neuroligin 1/NLGN1 was detected in perfusion fixed frozen sections of rat brain (cortex) using Sheep Anti‑Rat Neuroligin 1/ NLGN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4340) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to neuronal cell bodies, processes, and dendritic spines. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neuroligin 1/NLGN1
Neuroligin 1 (neurexin ligand 1; also NL1) is a 116 kDa member of the type-B carboxyesterase/lipase family of proteins. It is postsynaptically expressed on neurons and initiates excitatory presynapse maturation through binding to a select isoform of beta -neurexin. Mature rat NL1 is 798 amino acids (aa) in length. It is expressed as a type I transmembrane glycoprotein that contains a 652 aa extracellular domain (ECD) (aa 46‑697) and a 125 aa cytoplasmic tail. The ECD possesses a nonfunctional esterase homology domain (aa 54‑612). Rat NL1 has at least three alternate splice forms. One shows a deletion of aa 165‑184 plus aa 298‑306, while the remaining two show deletions of either aa 165‑184, or aa 298‑306, respectively. Rat ECD shares 97% and 93% aa identity with mouse and human NL1 CED, respectively.
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