Human/Rat PTK7/CCK4 Antibody

Catalog # Availability Size / Price Qty
MAB4499
MAB4499-SP
Detection of Human and Rat PTK7/CCK4 by Western Blot.
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Product Details
Citations (4)
FAQs
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Human/Rat PTK7/CCK4 Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat PTK7/CCK4 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 525222
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PTK7/CCK4
Ala31-Ser199
Accession # Q13308
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
20 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Rat PTK7/CCK4 antibody by Western Blot. View Larger

Detection of Human and Rat PTK7/CCK4 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat PTK7/CCK4 Monoclonal Antibody (Catalog # MAB4499) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTK7/CCK4 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry PTK7/CCK4 antibody in Human Colon Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

PTK7/CCK4 in Human Colon Cancer Tissue. PTK7/CCK4 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human/Rat PTK7/CCK4 Monoclonal Antibody (Catalog # MAB4499) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human PTK7/CCK4 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human PTK7/CCK4 by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTK7/CCK4 at approximately 168 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Rat PTK7/CCK4 Monoclonal Antibody (Catalog # MAB4499). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTK7/CCK4

Protein tyrosine kinase 7 (PTK7), also known as colon carcinoma kinase 4 (CCK4), is a receptor tyrosine kinase (RTK) involved in colon carcinoma development and/or proliferation. Similar to the RTK HER3, PTK7 contains a catalytically inactive tyrosine kinase domain, suggesting a potential tumor-characteristic role as a signal amplifier or modulator for an as yet unidentified kinase-competent partner. PTK7 may also play a role during gastrulation in the rodent embryo.

Long Name
Protein Tyrosine Kinase 7
Entrez Gene IDs
5754 (Human); 71461 (Mouse); 301242 (Rat)
Alternate Names
CCK4; CCK-4; CCK4Colon carcinoma kinase 4; colon carcinoma kinase-4; Protein-tyrosine kinase 7; Pseudo tyrosine kinase receptor 7; PTK7 protein tyrosine kinase 7; PTK7; Tyrosine-protein kinase-like 7

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Citations for Human/Rat PTK7/CCK4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Identification and targeting of protein tyrosine kinase 7 (PTK7) as an immunotherapy candidate for neuroblastoma
    Authors: Jasmine Y. Lee, Hunter C. Jonus, Arhanti Sadanand, Gianna M. Branella, Victor Maximov, Suttipong Suttapitugsakul et al.
    Cell Reports Medicine
  2. The Increased PTK7 Expression Is a Malignant Factor in Cervical Cancer
    Authors: JJ Sun, HL Li, SJ Guo, H Ma, SJ Liu, D Liu, FX Xue
    Dis. Markers, 2019-03-03;2019(0):5380197.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  3. miR‑205‑5p/PTK7 axis is involved in the proliferation, migration and invasion of colorectal cancer cells
    Authors: Shuo Chen, Yan Wang, Yinan Su, Lin Zhang, Mingqing Zhang, Xueqing Li et al.
    Molecular Medicine Reports
  4. Protein Tyrosine Kinase 7 (PTK7) as a Predictor of Lymph Node Metastases and a Novel Prognostic Biomarker in Patients with Prostate Cancer
    Authors: Hongtuan Zhang, Andi Wang, Shiyong Qi, Shang Cheng, Bing Yao, Yong Xu
    International Journal of Molecular Sciences

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