Human Reg1A/B Antibody

Catalog # Availability Size / Price Qty
MAB49371
MAB49371-SP
Reg1A in Human Pancreas.
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Human Reg1A/B Antibody Summary

Species Reactivity
Human
Specificity
Detects human Reg1A in ELISAs. 100% cross-reactivity with recombinant human (rh) Reg1B in Western blot is observed. In direct ELISAs no cross-reactivity with rhReg3A, recombinant mouse Reg1, or recombinant rat Reg1A is observed.
Source
Monoclonal Rat IgG2A Clone # 431211
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Reg1A 
Gln23-Asn166
Accession # P05451
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Reg1A
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Reg1A in Human Pancreas. View Larger

Reg1A in Human Pancreas. Reg1A was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human Reg1A Monoclonal Antibody (Catalog # MAB49371) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Reg1A/B

Reg1A, also known as PTP, PSP, and lithostathine, is a member of the Reg family of secreted proteins with a C-type lectin domain. Due to variable glycosylation, pancreatic Reg1A exists as multiple species of 16‑18 kDa. Reg1A promotes the maintenance and growth of pancreatic islet beta -cells and intestinal villi. It is up‑regulated in pancreatitis and some carcinomas. Reg1A is an antigenic target in autoimmune diabetes. Human Reg1A shares 65%‑68% aa sequence identity with mouse and rat Reg1A.

Reg1B, also known as secretory pancreatic stone protein 2 and lithostathine 1 beta, is a type I subclass member of the Reg family, which comprises secreted proteins with a C-type lectin domain. Reg1B is highly related to Reg1A, a protein that is implicated in islet cell regeneration and diabetogenesis.

Long Name
Regenerating Islet-derived 1A/B
Alternate Names
Reg1A/B

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Reg1A/B Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human Reg1A /B Antibody
By Anonymous on 05/17/2022
Application: IHC Sample Tested: Pancreas tissue Species: Human

Human Reg1A Antibody
By Anonymous on 06/09/2020
Application: ELISA Sample Tested: Serum and Plasma Species: Human

We used this antibody in an in-house ELISA along with pAb (AF4937) and protein standard to quantify Reg1 alpha in human serum and plasma. This combination detected Reg1alpha in human serum and plasma.