Human Relaxin R2 Antibody

Catalog # Availability Size / Price Qty
MAB102501-100
MAB102501-SP
Human Relaxin R2 Antibody in Flow Cytometry
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Human Relaxin R2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Relaxin R2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 1013903
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Relaxin R2
Met1-Asn416
Accession # Q8WXD0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Relaxin R2 in HEK293 Human Cell Line Transfected with Human Relaxin R2 and eGFP by Flow Cytometry. View Larger

Detection of Relaxin R2 in HEK293 Human Cell Line Transfected with Human Relaxin R2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human Relaxin R2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Relaxin R2 Monoclonal Antibody (Catalog # MAB102501) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Relaxin R2

Relaxin R2 (also known as LGR8) is the G-protein coupled receptor (GPCR) for Relaxin, and may also be a receptor for INSL3. LGR8 can mediate the action of Relaxin through cAMP-dependent pathway distinct from structurally related insulin and insulin-like growth factor family ligand. Distribution of this receptor implicates its role in reproductive, brain, renal, cardiovascular and other functions.

Long Name
Relaxin Receptor 2
Entrez Gene IDs
122042 (Human); 140498 (Mouse)
Alternate Names
G protein coupled receptor affecting testicular descent; Gpr106; GPR106G-protein coupled receptor 106; Great; GREATRelaxin family peptide receptor 2; INSL3R; leucine-rich repeat-containing G protein-coupled receptor 8; Leucine-rich repeat-containing G-protein coupled receptor 8; Lgr8; LGR8.1; LGR8G-protein coupled receptor affecting testicular descent; Relaxin R2; relaxin receptor 2; relaxin/insulin-like family peptide receptor 2; RelaxinR2; RLN2R; Rxfp2; RXFPR2

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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