Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 373722
View all Rex-1/ZFP42 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Rex-1
Ser2-Lys150
Accession # NP_777560

Specificity

Detects human Rex-1 in direct ELISAs and Western blots. Does not cross-react with recombinant human YY1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Rex‑1/ZFP42 Antibody

Rex‑1 antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC).

Rex‑1 in NTera-2 Human Cell Line.

Rex‑1 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using 10 µg/mL Human Rex‑1 Monoclonal Antibody (Catalog # MAB3598) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Rex‑1/ZFP42 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Human Rex‑1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Rex-1/ZFP42

Human Rex-1 (also Zfp-42) is a 35 kDa zinc finger family transcription factor that is highly expressed in human embryonic stem cells. It is one of several gene markers used to identify human stem cells. It is 310 amino acids in length and contains a zinc finger-binding domain between aa 214-307. Over this region, human and mouse show modest orthology at 47% aa identity.

Long Name

Zinc Finger Protein 42

Alternate Names

Rex1, ZFP42

Entrez Gene IDs

132625 (Human); 22702 (Mouse); 290765 (Rat)

Gene Symbol

ZFP42

UniProt

NP_777560

Additional Rex-1/ZFP42 Products

Product Documents for Human Rex‑1/ZFP42 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Rex‑1/ZFP42 Antibody

For research use only

Citations for Human Rex‑1/ZFP42 Antibody

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Protocols

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