Human Rex-1/ZFP42 Antibody

Catalog # Availability Size / Price Qty
MAB3598
MAB3598-SP
Rex‑1 in NTera-2 Human Cell Line.
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Human Rex-1/ZFP42 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Rex-1 in direct ELISAs and Western blots. Does not cross-react with recombinant human YY1.
Source
Monoclonal Rat IgG2A Clone # 373722
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Rex-1
Ser2-Lys150
Accession # NP_777560
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Rex‑1
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Rex‑1 antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC). View Larger

Rex‑1 in NTera-2 Human Cell Line. Rex‑1 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using 10 µg/mL Human Rex‑1 Monoclonal Antibody (Catalog # MAB3598) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rex-1/ZFP42

Human Rex-1 (also Zfp-42) is a 35 kDa zinc finger family transcription factor that is highly expressed in human embryonic stem cells. It is one of several gene markers used to identify human stem cells. It is 310 amino acids in length and contains a zinc finger-binding domain between aa 214-307. Over this region, human and mouse show modest orthology at 47% aa identity.

Long Name
Zinc Finger Protein 42
Entrez Gene IDs
132625 (Human); 22702 (Mouse); 290765 (Rat)
Alternate Names
FLJ20086; KIAA1584; MGC21637; MGC61687; Rex1; Rex-1; SUHW4; Suppressor of hairy wing homolog 4; ZFP42; zinc finger protein 280D; Zinc finger protein 634; ZNF634suppressor of hairy wing homolog 4 (Drosophila)

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