Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all Rex-1/ZFP42 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Rex‑1/ZFP42
Ser2-Lys150
Accession # NP_777560

Specificity

Detects human Rex‑1/ZFP42 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Rex-1/ZFP42 Biotinylated Antibody

Rex‑1 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC).

Rex‑1 in BG01V Human Stem Cells.

Rex‑1 was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Human Rex‑1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3598) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Rex-1/ZFP42 Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Western Blot

0.1 µg/mL
Sample: Recombinant Human Rex‑1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Rex-1/ZFP42

Rex-1, also named zinc finger protein 42 (Zfp-42), is a 32 kDa transcription factor belonging to the kruppel C2H2-type zinc-finger protein family. It is a putative stem cell marker and is highly expressed in embryonic and adult stem cells. Over the region used for immunization, human and mouse Rex-1 share 47% amino acid sequence identity.

Long Name

Zinc Finger Protein 42

Alternate Names

Rex1, ZFP42

Entrez Gene IDs

132625 (Human); 22702 (Mouse); 290765 (Rat)

Gene Symbol

ZFP42

UniProt

NP_777560

Additional Rex-1/ZFP42 Products

Product Documents for Human Rex-1/ZFP42 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Rex-1/ZFP42 Biotinylated Antibody

For research use only

Citations for Human Rex-1/ZFP42 Biotinylated Antibody

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Protocols

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