Human Rex-1/ZFP42 Biotinylated Antibody Summary
Accession # NP_777560
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Rex‑1 in BG01V Human Stem Cells. Rex‑1 was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Human Rex‑1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3598) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Rex-1, also named zinc finger protein 42 (Zfp-42), is a 32 kDa transcription factor belonging to the kruppel C2H2-type zinc-finger protein family. It is a putative stem cell marker and is highly expressed in embryonic and adult stem cells. Over the region used for immunization, human and mouse Rex-1 share 47% amino acid sequence identity.
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