Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Rex-1/ZFP42 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Rex‑1/ZFP42
Ser2-Lys150
Accession # NP_777560

Specificity

Detects human Rex‑1/ZFP42 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human YYI is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Rex‑1/ZFP42 Antibody

Rex-1/ZFP42 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC).

Rex‑1/ZFP42 in BG01V Human Stem Cells.

Rex-1/ZFP42 was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Sheep Anti-Human Rex-1/ZFP24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3598) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Rex-1/ZFP42 antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC).

Rex‑1/ZFP42 in NTera‑2 Human Cell Line.

Rex-1/ZFP42 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Sheep Anti-Human Rex-1/ZFP42 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3598) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Rex‑1/ZFP42 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human Rex‑1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Rex-1/ZFP42

Rex-1, also named zinc finger protein 42 (Zfp-42), is a 32 kDa transcription factor belonging to the kruppel C2H2-type zinc-finger protein family. It is a putative stem cell marker and is highly expressed in embryonic and adult stem cells. Over the region used for immunization, human and mouse Rex-1 share 47% amino acid sequence identity.

Long Name

Zinc Finger Protein 42

Alternate Names

Rex1, ZFP42

Entrez Gene IDs

132625 (Human); 22702 (Mouse); 290765 (Rat)

Gene Symbol

ZFP42

UniProt

NP_777560

Additional Rex-1/ZFP42 Products

Product Documents for Human Rex‑1/ZFP42 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Rex‑1/ZFP42 Antibody

For research use only

Citations for Human Rex‑1/ZFP42 Antibody

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Protocols

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