Human RIPK2/RIP2 Antibody

Catalog #: MAB103872 Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB103872-100
MAB103872-SP
Detection of Human RIPK2/RIP2 by Western Blot.
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Human RIPK2/RIP2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human RIPK2/RIP2 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2527A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E.coli-derived recombinant human RIPK2/RIP2
Met1-Met540
Accession # O43353
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

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Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
Ramos human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human RIPK2/RIP2 antibody by Western Blot. View Larger

Detection of Human RIPK2/RIP2 by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human RIPK2/RIP2 Monoclonal Antibody (Catalog # MAB103872) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for RIPK2/RIP2 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RIPK2/RIP2

Receptor-interacting serine/threonine-protein kinase 2, also known as RIPK2 or RIP-2, is a  540 amino acids (aa) Serine/threonine/tyrosine kinase encoded by the RIPK2 gene. RIP-2, a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, plays an essential role in modulation of innate and adaptive immune responses. Human RIP2 is an adapter protein necessary for signal propagation of the Nucleotide-binding-oligomerization-domain-containing proteins 1/2 (NOD1 and NOD2). Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize, and recruit RIPK2 through the C-terminal caspase recruitment domain (CARD). Human and mouse RIPK2 aa sequences are 84% identical .

Long Name
Receptor Interacting Serine/Threonine Kinase 2
Entrez Gene IDs
8767 (Human); 192656 (Mouse)
Alternate Names
CARD3; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase; CARD-containing interleukin-1 beta-converting enzyme-associated kinase; CARDIAKCCK; EC 2.7.11; EC 2.7.11.1; GIG30; growth-inhibiting gene 30; receptor interacting protein 2; receptor-interacting protein (RIP)-like interacting caspase-like apoptosisregulatory protein (CLARP) kinase; Receptor-interacting protein 2; receptor-interacting serine/threonine-protein kinase 2; receptor-interacting serine-threonine kinase 2; RICK; RICKGIG30; RIP2; RIP-2; RIP2CARD-carrying kinase; RIPK2; RIP-like-interacting CLARP kinase

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