Human RUNX2/CBFA1 Antibody

(12 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human RUNX2/CBFA1.
  • Source
    Monoclonal Rat IgG2B Clone # 232902
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human RUNX2/CBFA1 isoform 2
    Lys233-Tyr418
    Accession # Q13950
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
RUNX2/CBFA1 in U2OS Human Cell Line. RUNX2/CBFA1 was detected in immersion fixed U2OS human osteosarcoma cell line using Rat Anti-Human RUNX2/CBFA1 Monoclonal Antibody (Catalog # MAB2006) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RUNX2/CBFA1

CBFA1, also called runt-related transcription factor 2 (RUNX2), is an essential transcription factor for the regulation of osteoblast differentiation (1). The CBFA1 gene potentially encodes several proteins that differ in their N-terminal sequences and transactivation capacities (2).

  • References:
    1. Ducy, P. et al. (1997) Cell 89:747.
    2. Xiao, Z.S. et al. (1998) Gene 214:187.
    3. Sato, M. et al. (1998) Oncogene 17:1517.
  • Long Name:
    Runt-related Transcription Factor 2
  • Entrez Gene IDs:
    860 (Human)
  • Alternate Names:
    Acute myeloid leukemia 3 protein; AML3CCD; CBFA1; CBFA1MGC120022; Core-binding factor subunit alpha-1; MGC120023; PEA2-alpha A; PEBP2A; PEBP2A1CCD1; PEBP2aA1; PEBP2-alpha A; runt domain, alpha subunit 1; runt-related transcription factor 2; RUNX2; SL3/AKV core-binding factor alpha A subunit; SL3-3 enhancer factor 1 alpha A subunit
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Cell-matrix signals specify bone endothelial cells during developmental osteogenesis
    Authors: UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams
    Nat. Cell Biol, 2017;19(3):189-201.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Collagen type XV and the 'osteogenic status'
    Authors: G Lisignoli, E Lambertini, C Manferdini, E Gabusi, L Penolazzi, F Paolella, M Angelozzi, V Casagranda, R Piva
    J. Cell. Mol. Med, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
    Authors: Ramasamy S, Kusumbe A, Wang L, Adams R
    Nature, 2014;507(7492):376-80.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. GATA4 is essential for bone mineralization via ERalpha and TGFbeta/BMP pathways.
    Authors: Guemes M, Garcia A, Rigueur D, Runke S, Wang W, Zhao G, Mayorga V, Atti E, Tetradis S, Peault B, Lyons K, Miranda-Carboni G, Krum S
    J Bone Miner Res, 2014;29(12):2676-87.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Paraffin-embedded
  5. Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone.
    Authors: Kusumbe A, Ramasamy S, Adams R
    Nature, 2014;507(7492):323-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  6. Identification of a clonally expanding haematopoietic compartment in bone marrow.
    Authors: Wang L, Benedito R, Bixel M, Zeuschner D, Stehling M, Savendahl L, Haigh J, Snippert H, Clevers H, Breier G, Kiefer F, Adams R
    EMBO J, 2013;32(2):219-30.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fluorescence
  7. Elastin degradation and vascular smooth muscle cell phenotype change precede cell loss and arterial medial calcification in a uremic mouse model of chronic kidney disease.
    Authors: Pai A, Leaf EM, El-Abbadi M, Giachelli CM
    Am. J. Pathol., 2011;178(2):764-73.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. Distinct transcriptional profiles characterize bone microenvironment mesenchymal cells rather than osteoblasts in relationship with multiple myeloma bone disease.
    Authors: Todoerti K, Lisignoli G, Storti P, Agnelli L, Novara F, Manferdini C, Codeluppi K, Colla S, Crugnola M, Abeltino M, Bolzoni M, Sgobba V, Facchini A, Lambertenghi-Deliliers G, Zuffardi O, Rizzoli V, Neri A, Giuliani N
    Exp. Hematol., 2010;38(2):141-53.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  9. Phosphate feeding induces arterial medial calcification in uremic mice: role of serum phosphorus, fibroblast growth factor-23, and osteopontin.
    Authors: El-Abbadi MM, Pai AS, Leaf EM, Yang HY, Bartley BA, Quan KK, Ingalls CM, Liao HW, Giachelli CM
    Kidney Int., 2009;75(12):1297-307.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  10. Emergence of patterned stem cell differentiation within multicellular structures.
    Authors: Ruiz SA, Chen CS
    Stem Cells, 2008;26(11):2921-7.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  11. Estrogen protects bone by inducing Fas ligand in osteoblasts to regulate osteoclast survival.
    Authors: Krum SA, Miranda-Carboni GA, Hauschka PV, Carroll JS, Lane TF, Freedman LP, Brown M
    EMBO J., 2008;27(3):535-45.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  12. The guidance of human mesenchymal stem cell differentiation in vitro by controlled modifications to the cell substrate.
    Authors: Curran JM, Chen R, Hunt JA
    Biomaterials, 2006;27(27):4783-93.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 12 Citations
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