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Human RUNX2/CBFA1 Antibody

Catalog #: MAB2006
20 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2006
MAB2006-SP
RUNX2/CBFA1 in U2OS Human Cell Line.
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Product Details
Citations (20)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human RUNX2/CBFA1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human RUNX2/CBFA1.
Source
Monoclonal Rat IgG2B Clone # 232902
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human RUNX2/CBFA1 isoform 2
Lys233-Tyr418
Accession # Q13950
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry RUNX2/CBFA1 antibody in U2OS Human Cell Line by Immunocytochemistry (ICC). View Larger

RUNX2/CBFA1 in U2OS Human Cell Line. RUNX2/CBFA1 was detected in immersion fixed U2OS human osteosarcoma cell line using Rat Anti-Human RUNX2/CBFA1 Monoclonal Antibody (Catalog # MAB2006) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RUNX2/CBFA1

CBFA1, also called runt-related transcription factor 2 (RUNX2), is an essential transcription factor for the regulation of osteoblast differentiation (1). The CBFA1 gene potentially encodes several proteins that differ in their N-terminal sequences and transactivation capacities (2).

References
  1. Ducy, P. et al. (1997) Cell 89:747.
  2. Xiao, Z.S. et al. (1998) Gene 214:187.
  3. Sato, M. et al. (1998) Oncogene 17:1517.
Long Name
Runt-related Transcription Factor 2
Entrez Gene IDs
860 (Human)
Alternate Names
Acute myeloid leukemia 3 protein; CBFA1; CBF-alpha-1; CCD1; CCDAML3; CLCD; Core-binding factor subunit alpha-1; core-binding factor, runt domain, alpha subunit 1; MGC120023; ML3; oncogene AML-3; OSF2; OSF-2; osteoblast-specific transcription factor 2; PEA2aA; PEA2-alpha A; PEBP2A; PEBP2aA; PEBP2-alpha A; polyomavirus enhancer-binding protein 2 alpha A subunit; runt domain, alpha subunit 1; runt related transcription factor 2; runt-related transcription factor 2; RUNX2; SL3/AKV core-binding factor alpha A subunit; SL3-3 enhancer factor 1 alpha A subunit

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Citations for Human RUNX2/CBFA1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Proteomic analysis reveals key differences between squamous cell carcinomas and adenocarcinomas across multiple tissues
    Authors: Q Song, Y Yang, D Jiang, Z Qin, C Xu, H Wang, J Huang, L Chen, R Luo, X Zhang, Y Huang, L Xu, Z Yu, S Tan, M Deng, R Xue, J Qie, K Li, Y Yin, X Yue, X Sun, J Su, F He, C Ding, Y Hou
    Oncogene, 2022;13(1):4167.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Induction of osteogenesis by bone-targeted Notch activation
    Authors: C Xu, VV Dinh, K Kruse, HW Jeong, EC Watson, S Adams, F Berkenfeld, M Stehling, SJ Rasouli, R Fan, R Chen, I Bedzhov, Q Chen, K Kato, ME Pitulescu, RH Adams
    Elife, 2022;11(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Pleiotropic Roles of NOTCH1 Signaling in the Loss of Maturational Arrest of Human Osteoarthritic Chondrocytes
    Authors: M Minguzzi, V Panichi, S D'Adamo, S Cetrullo, L Cattini, F Flamigni, E Mariani, RM Borzì
    International Journal of Molecular Sciences, 2021;22(21):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Shifting osteogenesis in vascular calcification
    Authors: J Yao, X Wu, X Qiao, D Zhang, L Zhang, JA Ma, X Cai, KI Boström, Y Yao
    JCI Insight, 2021;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Pericytes as a Source of Osteogenic Cells in Bone Fracture Healing
    Authors: S Supakul, K Yao, H Ochi, T Shimada, K Hashimoto, S Sunamura, Y Mabuchi, M Tanaka, C Akazawa, T Nakamura, A Okawa, S Takeda, S Sato
    Int J Mol Sci, 2019;20(5):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. Osteogenic Potential of Human Umbilical Cord Mesenchymal Stem Cells on Coralline Hydroxyapatite/Calcium Carbonate Microparticles
    Authors: AGE Day, WR Francis, K Fu, IL Pieper, O Guy, Z Xia
    Stem Cells Int, 2018;2018(0):4258613.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Cell-matrix signals specify bone endothelial cells during developmental osteogenesis
    Authors: UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams
    Nat. Cell Biol, 2017;19(3):189-201.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Collagen type XV and the 'osteogenic status'
    Authors: G Lisignoli, E Lambertini, C Manferdini, E Gabusi, L Penolazzi, F Paolella, M Angelozzi, V Casagranda, R Piva
    J. Cell. Mol. Med, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. The Selective Serotonin Re-Uptake Inhibitor Fluoxetine Directly Inhibits Osteoblast Differentiation and Mineralization During Fracture Healing in Mice
    Authors: P Leucht
    J. Bone Miner. Res., 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
    Authors: Ramasamy S, Kusumbe A, Wang L, Adams R
    Nature, 2014;507(7492):376-80.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. GATA4 is essential for bone mineralization via ERalpha and TGFbeta/BMP pathways.
    Authors: Guemes M, Garcia A, Rigueur D, Runke S, Wang W, Zhao G, Mayorga V, Atti E, Tetradis S, Peault B, Lyons K, Miranda-Carboni G, Krum S
    J Bone Miner Res, 2014;29(12):2676-87.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-P
  12. Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone.
    Authors: Kusumbe A, Ramasamy S, Adams R
    Nature, 2014;507(7492):323-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  13. Identification of a clonally expanding haematopoietic compartment in bone marrow.
    Authors: Wang L, Benedito R, Bixel M, Zeuschner D, Stehling M, Savendahl L, Haigh J, Snippert H, Clevers H, Breier G, Kiefer F, Adams R
    EMBO J, 2013;32(2):219-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Elastin degradation and vascular smooth muscle cell phenotype change precede cell loss and arterial medial calcification in a uremic mouse model of chronic kidney disease.
    Authors: Pai A, Leaf EM, El-Abbadi M, Giachelli CM
    Am. J. Pathol., 2011;178(2):764-73.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Distinct transcriptional profiles characterize bone microenvironment mesenchymal cells rather than osteoblasts in relationship with multiple myeloma bone disease.
    Authors: Todoerti K, Lisignoli G, Storti P, Agnelli L, Novara F, Manferdini C, Codeluppi K, Colla S, Crugnola M, Abeltino M, Bolzoni M, Sgobba V, Facchini A, Lambertenghi-Deliliers G, Zuffardi O, Rizzoli V, Neri A, Giuliani N
    Exp. Hematol., 2010;38(2):141-53.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Phosphate feeding induces arterial medial calcification in uremic mice: role of serum phosphorus, fibroblast growth factor-23, and osteopontin.
    Authors: El-Abbadi MM, Pai AS, Leaf EM, Yang HY, Bartley BA, Quan KK, Ingalls CM, Liao HW, Giachelli CM
    Kidney Int., 2009;75(12):1297-307.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. Emergence of patterned stem cell differentiation within multicellular structures.
    Authors: Ruiz SA, Chen CS
    Stem Cells, 2008;26(11):2921-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Estrogen protects bone by inducing Fas ligand in osteoblasts to regulate osteoclast survival.
    Authors: Krum SA, Miranda-Carboni GA, Hauschka PV, Carroll JS, Lane TF, Freedman LP, Brown M
    EMBO J., 2008;27(3):535-45.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. The guidance of human mesenchymal stem cell differentiation in vitro by controlled modifications to the cell substrate.
    Authors: Curran JM, Chen R, Hunt JA
    Biomaterials, 2006;27(27):4783-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. Lack of Endothelial Nitric Oxide Synthase Accelerates Ectopic Calcification in Uremic Mice Fed an Adenine and High Phosphorus Diet.
    Authors: Oe Y, Mitsui S, Sato E, Shibata N, Kisu K, Sekimoto A, Miyazaki M, Sato H, Ito S, Takahashi N
    Am J Pathol, 0;191(2):283-293.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

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Reviews for Human RUNX2/CBFA1 Antibody

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Human RUNX2/CBFA1 Antibody
By Abby Sukarto on 02/13/2020
Application: Flow Sample Tested: Bone Extracts Species: Human
Flow Cytometry Human RUNX2/CBFA1 Antibody MAB2006
Human RUNX2/CBFA1 Antibody
By Anonymous on 08/31/2018
Application: ICC/IF Sample Tested: bone marrow Species: Mouse
Immunocytochemistry/Immunofluorescence Human RUNX2/CBFA1 Antibody MAB2006