Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 232902
View all RUNX2/CBFA1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human RUNX2/CBFA1 isoform 2
Lys233-Tyr418
Accession # Q13950

Specificity

Detects human RUNX2/CBFA1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human RUNX2/CBFA1 Antibody

RUNX2/CBFA1 antibody in U2OS Human Cell Line by Immunocytochemistry (ICC).

RUNX2/CBFA1 in U2OS Human Cell Line.

RUNX2/CBFA1 was detected in immersion fixed U2OS human osteosarcoma cell line using Rat Anti-Human RUNX2/CBFA1 Monoclonal Antibody (Catalog # MAB2006) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human RUNX2/CBFA1 by Immunohistochemistry

Detection of Human RUNX2/CBFA1 by Immunohistochemistry

Proteomic differences of common SCCs&rare SCCs.j Immunohistochemistry staining for RUNX2, FOXO1,&PLIN1 expression in rare SCCs (one case of thyroid SCC&one case of pancreatic SCC) was concordant with the mass spectrometry findings. Scale bar, 100 μm. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35851595), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of RUNX2/CBFA1 by Western Blot

Detection of RUNX2/CBFA1 by Western Blot

The activated Wnt signalling pathway affects chondrogenic differentiation of Cesca KEGG analysis of bulk RNA-seq at different induction points. b The transcription factor SOX9, condylar chondrocyte anabolism markers COL2A1 and COL1A1, and the catabolism marker MMP13 were obviously upregulated 3 days after the removal of CHIR99021. c An obvious downregulation of beta -catenin was detected by western blotting 7 days after the removal of CHIR99021. d The chondrogenic transcription factors SOX9 and RUNX2 were decreased, as well as COL2A1 and OPN, while MMP13 was enhanced in the presence of CHIR99021 in N2B27. e A scheme for the Wnt signalling pathway involved in chondrogenic differentiation of ECCs. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36477591), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of RUNX2/CBFA1 by Western Blot

Detection of RUNX2/CBFA1 by Western Blot

The activated Wnt signalling pathway affects chondrogenic differentiation of Cesca KEGG analysis of bulk RNA-seq at different induction points. b The transcription factor SOX9, condylar chondrocyte anabolism markers COL2A1 and COL1A1, and the catabolism marker MMP13 were obviously upregulated 3 days after the removal of CHIR99021. c An obvious downregulation of beta -catenin was detected by western blotting 7 days after the removal of CHIR99021. d The chondrogenic transcription factors SOX9 and RUNX2 were decreased, as well as COL2A1 and OPN, while MMP13 was enhanced in the presence of CHIR99021 in N2B27. e A scheme for the Wnt signalling pathway involved in chondrogenic differentiation of ECCs. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36477591), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of RUNX2/CBFA1 by Western Blot

Detection of RUNX2/CBFA1 by Western Blot

The activated Wnt signalling pathway affects chondrogenic differentiation of Cesca KEGG analysis of bulk RNA-seq at different induction points. b The transcription factor SOX9, condylar chondrocyte anabolism markers COL2A1 and COL1A1, and the catabolism marker MMP13 were obviously upregulated 3 days after the removal of CHIR99021. c An obvious downregulation of beta -catenin was detected by western blotting 7 days after the removal of CHIR99021. d The chondrogenic transcription factors SOX9 and RUNX2 were decreased, as well as COL2A1 and OPN, while MMP13 was enhanced in the presence of CHIR99021 in N2B27. e A scheme for the Wnt signalling pathway involved in chondrogenic differentiation of ECCs. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36477591), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of RUNX2/CBFA1 by Western Blot

Detection of RUNX2/CBFA1 by Western Blot

CSSEDF-expanded cells stably self-renewed and shared markers of CPCs/C.S.S. A single CSSEDF-expanded cell (passage 5) colony on a Matrigel-coated surface. Scale bars, 50 µm. b–d Immunocytochemistry showing that CSSEDF-expanded cells expressed genes identified as neural ectodermal markers, including SIX1, NESTIN and ETS1. Scale bars, 50 µm. e–i Immunocytochemistry showing that CSSEDF-expanded cells (passage 5) expressed genes identified as CPC/CSC markers, including SOX9, RUNX2, SOX5, TWIST1, and CD29. Scale bars, 50 µm. j–l Immunocytochemistry showing that CSSEDF-expanded cells (passage 5) express genes recently identified as TMJ condylar cartilage markers, including FOXC1, FOXC2 and MSX1. Scale bars, 50 µm. m Flow cytometry analysis showing that CSSEDF-expanded cells stably express cell proliferation and CPC/CSC markers after long-term in vitro expansion. n Gene expression of chondrocytic lineage markers by CSSEDF-expanded cells at different passages and patient TMJ condylar cartilage were analysed by PCR. CPCs/CSCs: cartilaginous progenitor cells/cartilaginous stem cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36477591), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of RUNX2/CBFA1 by Western Blot

Detection of RUNX2/CBFA1 by Western Blot

CSSEDF-expanded cells stably self-renewed and shared markers of CPCs/C.S.S. A single CSSEDF-expanded cell (passage 5) colony on a Matrigel-coated surface. Scale bars, 50 µm. b–d Immunocytochemistry showing that CSSEDF-expanded cells expressed genes identified as neural ectodermal markers, including SIX1, NESTIN and ETS1. Scale bars, 50 µm. e–i Immunocytochemistry showing that CSSEDF-expanded cells (passage 5) expressed genes identified as CPC/CSC markers, including SOX9, RUNX2, SOX5, TWIST1, and CD29. Scale bars, 50 µm. j–l Immunocytochemistry showing that CSSEDF-expanded cells (passage 5) express genes recently identified as TMJ condylar cartilage markers, including FOXC1, FOXC2 and MSX1. Scale bars, 50 µm. m Flow cytometry analysis showing that CSSEDF-expanded cells stably express cell proliferation and CPC/CSC markers after long-term in vitro expansion. n Gene expression of chondrocytic lineage markers by CSSEDF-expanded cells at different passages and patient TMJ condylar cartilage were analysed by PCR. CPCs/CSCs: cartilaginous progenitor cells/cartilaginous stem cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36477591), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of RUNX2/CBFA1 by Western Blot

Detection of RUNX2/CBFA1 by Western Blot

The activated Wnt signalling pathway affects chondrogenic differentiation of Cesca KEGG analysis of bulk RNA-seq at different induction points. b The transcription factor SOX9, condylar chondrocyte anabolism markers COL2A1 and COL1A1, and the catabolism marker MMP13 were obviously upregulated 3 days after the removal of CHIR99021. c An obvious downregulation of beta -catenin was detected by western blotting 7 days after the removal of CHIR99021. d The chondrogenic transcription factors SOX9 and RUNX2 were decreased, as well as COL2A1 and OPN, while MMP13 was enhanced in the presence of CHIR99021 in N2B27. e A scheme for the Wnt signalling pathway involved in chondrogenic differentiation of ECCs. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36477591), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human RUNX2/CBFA1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed U2OS human osteosarcoma cell line

Reviewed Applications

Read 3 reviews rated 4 using MAB2006 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RUNX2/CBFA1

CBFA1, also called runt-related transcription factor 2 (RUNX2), is an essential transcription factor for the regulation of osteoblast differentiation (1). The CBFA1 gene potentially encodes several proteins that differ in their N-terminal sequences and transactivation capacities (2).

References

  1. Ducy, P. et al. (1997) Cell 89:747.
  2. Xiao, Z.S. et al. (1998) Gene 214:187.
  3. Sato, M. et al. (1998) Oncogene 17:1517.

Long Name

Runt-related Transcription Factor 2

Alternate Names

CBFA1

Entrez Gene IDs

860 (Human)

Gene Symbol

RUNX2

UniProt

Q13950

Additional RUNX2/CBFA1 Products

Product Documents for Human RUNX2/CBFA1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human RUNX2/CBFA1 Antibody

For research use only

Citations for Human RUNX2/CBFA1 Antibody

Customer Reviews for Human RUNX2/CBFA1 Antibody (3)

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3 Customer Ratings
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human RUNX2/CBFA1 Antibody
    Name: Abby Sukarto
    Application: Flow Cytometry
    Sample Tested: Bone Extracts
    Species: Human
    Verified Customer | Posted 02/13/2020
    Human RUNX2/CBFA1 Antibody MAB2006
  • Human RUNX2/CBFA1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: bone marrow
    Species: Mouse
    Verified Customer | Posted 08/31/2018
    Human RUNX2/CBFA1 Antibody MAB2006
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 22034088
    Species: Human
    Verified Customer | Posted 02/16/2015

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