Human Serpin A7/TBG Antibody

Catalog # Availability Size / Price Qty
AF8176
AF8176-SP
Detection of Human Serpin A7/TBG by Western Blot.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human Serpin A7/TBG Antibody Summary

Species Reactivity
Human
Specificity
Detects human Serpin A7/TBG in direct ELISAs and Western blots.  In direct ELISAs, approximately 5% cross-reactivity with recombinant human Serpin A9 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Serpin A7/TBG
Ala21-Ala415
Accession # P05543
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Serpin A7/TBG by Western Blot. View Larger

Detection of Human Serpin A7/TBG by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Serpin A7/TBG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8176) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Serpin A7/TBG at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A7/TBG

Serpin A7 (also TBG/Thyroxine-binding globulin and T4-binding globulin) is a secreted 54 kDa glycoprotein, non-inhibitory member of the serpin family of proteins. It is expressed by the liver and secreted in plasma. Mutations in the SERPINA7 gene can result in a whole spectrum of deficiencies, characterized by either reduced or increased TBG levels in the serum. A deficiency in TBG result in low serum total T4 and T3 count in clinically euthyroid subjects in the presence of normal serum TSH. Over aa21-415, human serpin A7 shares 76% aa sequence identity with mouse serpin A7.        

Entrez Gene IDs
6906 (Human); 331535 (Mouse); 81806 (Rat)
Alternate Names
antitrypsin), member 7; FLJ17820; member 7; Serpin A7; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin); T4-binding globulin; TBG; TBGclade A (alpha-1 antiproteinase; thyroxin-binding globulin; thyroxine-binding globulin

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Serpin A7/TBG Antibody

There are currently no reviews for this product. Be the first to review Human Serpin A7/TBG Antibody and earn rewards!

Have you used Human Serpin A7/TBG Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review