Human Serpin A7/TBG Antibody

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Detection of Human Serpin A7/TBG by Western Blot.
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Product Details
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Human Serpin A7/TBG Antibody Summary

Species Reactivity
Detects human Serpin A7/TBG in ELISA and Western Blot.
Monoclonal Mouse IgG1 Clone # 893339
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human Serpin A7/TBG
Accession # P05543
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Serpin A7/TBG antibody by Western Blot. View Larger

Detection of Human Serpin A7/TBG by Western Blot. Western blot shows human plasma PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serpin A7/TBG Monoclonal Antibody (Catalog # MAB8176) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin A7/TBG at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A7/TBG

Serpin A7 (also TBG/Thyroxine-binding globulin and T4-binding globulin) is a secreted 54 kDa glycoprotein, non-inhibitory member of the serpin family of proteins. It is expressed by the liver and secreted in plasma. Mutations in the SERPINA7 gene can result in a whole spectrum of deficiencies, characterized by either reduced or increased TBG levels in the serum. A deficiency in TBG result in low serum total T4 and T3 count in clinically euthyroid subjects in the presence of normal serum TSH. Over aa21-415, human serpin A7 shares 76% aa sequence identity with mouse serpin A7.        

Entrez Gene IDs
6906 (Human); 331535 (Mouse); 81806 (Rat)
Alternate Names
antitrypsin), member 7; FLJ17820; member 7; Serpin A7; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin); T4-binding globulin; TBG; TBGclade A (alpha-1 antiproteinase; thyroxin-binding globulin; thyroxine-binding globulin

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