Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Human

Applications

Knockout Validated, Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 930109
View all Serpin B2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Serpin B2
Glu2-Ala65, Ala99-Pro415
Accession # P05120

Specificity

Detects human Serpin B2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Serpin B2 Antibody

Detection of Human Serpin B2 antibody by Western Blot.

Detection of Human Serpin B2 by Western Blot.

Western blot shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with PMA and HEK001 human epidermal keratinocyte cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB8550) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin B2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Serpin B2 antibody in U937 Human Cell Line antibody by Flow Cytometry.

Detection of Serpin B2 in U937 Human Cell Line by Flow Cytometry.

U937 human histiocytic lymphoma cell line either untreated (upper panel) or treated with PMA and Calcium Ionomycin for 2 days (lower panel) was stained with Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB8550, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Serpin B2 antibody in U937 Human Cell Line by Immunocytochemistry (ICC).

Serpin B2 in U937 Human Cell Line.

Serpin B2 was detected in immersion fixed U937 human histiocytic lymphoma cell line stimulated with PMA using Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB8550) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Serpin B2 Antibody Specificity is Shown by Immunocytochemistry antibody in Knockout Cell Line.

Serpin B2 Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

Serpin B2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line treated with PMA but is not detected in Serpin B2 knockout (KO) K562 cell line using Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB8550) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Serpin B2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: U937 human histiocytic lymphoma cell line treated with PMA and Calcium Ionomycin

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line stimulated with PMA 

Knockout Validated

Serpin B2 is specifically detected in K562 human chronic myelogenous leukemia parental cell line but is not detectable in Serpin B2 knockout K562 cell line.

Western Blot

2 µg/mL
Sample: U937 human histiocytic lymphoma cell line treated with PMA and HEK001 human epidermal keratinocyte cell line

Reviewed Applications

Read 1 review rated 4 using MAB8550 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin B2

Serpin B2, also known as Plasminogen Activator Inhibitor 2 (PAI-2), is a 415 amino acid serine-type endopeptidase inhibitor with high expression in blood, vasculature and placenta. Its expression is upregulated in pregnancy and in activated monocytes/macrophages by a wide range of viral, bacterial and parasitic agents. Serpin B2 is upregulated in monocytes from HIV-1 infected patients, and is thought to modulate Th1/Th2 responses. Serpin B2/PAI-2 colocalizes with CHL1 and Vitronectin and mediates neurite outgrowth during post-natal brain development. Inhibition of uPA by SerpinB2 in tumors is associated with a favorable prognosis.

Alternate Names

PAI2, PLANH2, Urokinase Inhibitor

Entrez Gene IDs

5055 (Human); 18788 (Mouse); 60325 (Rat)

Gene Symbol

SERPINB2

UniProt

P05120

Additional Serpin B2 Products

Product Documents for Human Serpin B2 Antibody

Certificate of Analysis

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Product Specific Notices for Human Serpin B2 Antibody

For research use only

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  • Human Serpin B2 Antibody
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: Brain (trigeminal ganglia)
    Species: Mouse
    Verified Customer | Posted 06/04/2019

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Protocols

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