Human Serpin B2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Serpin B2 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 930109
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Serpin B2
    Glu2-Ala65, Ala99-Pro415
    Accession # P05120
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Knockout Validated

    Serpin B2 is specifically detected in K562 human chronic myelogenous leukemia parental cell line but is not detectable in Serpin B2 knockout K562 cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Serpin B2 by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with PMA and HEK001 human epidermal keratinocyte cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB8550) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin B2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Serpin B2 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line either untreated (upper panel) or treated with PMA and Calcium Ionomycin for 2 days (lower panel) was stained with Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB8550, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Immunocytochemistry
Serpin B2 in U937 Human Cell Line. Serpin B2 was detected in immersion fixed U937 human histiocytic lymphoma cell line stimulated with PMA using Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB8550) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Knockout Validated
Serpin B2 Specificity is Shown by Immunocytochemistry in Knockout Cell Line. Serpin B2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line treated with PMA but is not detected in Serpin B2 knockout (KO) K562 cell line using Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB8550) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin B2

Serpin B2, also known as Plasminogen Activator Inhibitor 2 (PAI-2), is a 415 amino acid serine-type endopeptidase inhibitor with high expression in blood, vasculature and placenta. Its expression is upregulated in pregnancy and in activated monocytes/macrophages by a wide range of viral, bacterial and parasitic agents. Serpin B2 is upregulated in monocytes from HIV-1 infected patients, and is thought to modulate Th1/Th2 responses. Serpin B2/PAI-2 colocalizes with CHL1 and Vitronectin and mediates neurite outgrowth during post-natal brain development. Inhibition of uPA by SerpinB2 in tumors is associated with a favorable prognosis.

  • Entrez Gene IDs:
    5055 (Human); 18788 (Mouse); 60325 (Rat)
  • Alternate Names:
    HsT1201; Monocyte Arg-serpin; PAI; PAI2; PAI2Urokinase inhibitor; Placental plasminogen activator inhibitor; PLANH2; PLANH2PAI-2; plasminogen activator inhibitor 2; plasminogen activator inhibitor, type II (arginine-serpin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2; Serpin B2; serpin peptidase inhibitor, clade B (ovalbumin), member 2; Urokinase Inhibitor
Related Research Areas
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