Human SH2D2A Antibody Summary
Accession # Q9NP31
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human SH2D2A by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line (untreated), human primary differentiated Th2 cells and human primary differentiated Th17 cells treated (+) with 1 µg/mL Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484) and 5 µg/mL Rat Anti-Mouse CD28 Monoclonal Antibody (Catalog # MAB4831) for 120 minutes. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SH2D2A Polyclonal Antibody (Catalog # AF6020) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SH2D2A at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human SH2D2A by Simple WesternTM.
Simple Western lane view shows lysate of human primary differentiated Th2 cells loaded at 0.2 mg/mL. A specific band was detected for SH2D2A at approximately 58 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human SH2D2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6020) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
SH2D2A (SH2 domain-containing protein 2A; also TSAd and VRAP) is a 52 kDa protein best described as a multifunctional intracellular adaptor molecule. It is expressed in activated CD4+ and CD8+ T cells, endothelial cells, and fibroblasts. SH2D2A is found in both cytoplasm and nucleus, and has multiple binding partners. After TCR activation, SH2D2A interacts with LCK, prolonging its activity. It also binds to SMAD2 and SMAD3, and constitutively interacts with PI3K and PLC gamma, thus participating in VEGF:KDR signaling. Human SH2D2A is 389 amino acids (aa) in length and contains one SH2 domain (aa 95‑186) and a Pro-rich region (aa 187‑389). Phosphorylation occurs on Y39, Y216 and Y305, and on S217 and S296.
Citations for Human SH2D2A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Axl is essential for VEGF-A-dependent activation of PI3K/Akt.
Authors: Ruan GX, Kazlauskas A,
EMBO J., 2012;31(7):1692-703.
Sample Types: Cell Lysates
VEGFR2 induces c-Src signaling and vascular permeability in vivo via the adaptor protein TSAd.
Authors: Sun Z, Li X, Massena S, Kutschera S, Padhan N, Gualandi L, Sundvold-Gjerstad V, Gustafsson K, Choy WW, Zang G, Quach M, Jansson L, Phillipson M, Abid MR, Spurkland A, Claesson-Welsh L
J. Exp. Med., 2012;209(7):1363-77.
Sample Types: Cell Lysates
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