Human SH2D2A Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human SH2D2A in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human SH2D2A
    Ile279-Gln389
    Accession # Q9NP31
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human SH2D2A by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line (untreated), human primary differentiated Th2 cells and human primary differentiated Th17 cells treated (+) with 1 µg/mL Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484) and 5 µg/mL Rat Anti-Mouse CD28 Monoclonal Antibody (Catalog # MAB4831) for 120 minutes. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SH2D2A Polyclonal Antibody (Catalog # AF6020) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SH2D2A at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human SH2D2A by Simple WesternTM. Simple Western lane view shows lysate of human primary  differentiated Th2 cells loaded at 0.2 mg/mL. A specific band was detected for SH2D2A at approximately 58 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human SH2D2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6020) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SH2D2A
SH2D2A (SH2 domain-containing protein 2A; also TSAd and VRAP) is a 52 kDa protein best described as a multifunctional intracellular adaptor molecule. It is expressed in activated CD4+ and CD8+ T cells, endothelial cells, and fibroblasts. SH2D2A is found in both cytoplasm and nucleus, and has multiple binding partners. After TCR activation, SH2D2A interacts with LCK, prolonging its activity. It also binds to SMAD2 and SMAD3, and constitutively interacts with PI3K and PLC gamma, thus participating in VEGF:KDR signaling. Human SH2D2A is 389 amino acids (aa) in length and contains one SH2 domain (aa 95‑186) and a Pro-rich region (aa 187‑389). Phosphorylation occurs on Y39, Y216 and Y305, and on S217 and S296.
  • Entrez Gene IDs:
    9047 (Human); 27371 (Mouse); 310688 (Rat)
  • Alternate Names:
    F2771; RIBP; SCAP; SH2 domain containing 2A; SH2 domain protein 2A; SH2 domain-containing adapter protein; SH2D2A; T cell specific adapter protein TSAd; T cell-specific adapter protein; T lymphocyte specific adaptor protein; TSAd; TSADSH2 domain-containing protein 2A; VEGF receptor-associated protein; VRAP
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. VEGFR2 induces c-Src signaling and vascular permeability in vivo via the adaptor protein TSAd.
    Authors: Sun Z, Li X, Massena S, Kutschera S, Padhan N, Gualandi L, Sundvold-Gjerstad V, Gustafsson K, Choy WW, Zang G, Quach M, Jansson L, Phillipson M, Abid MR, Spurkland A, Claesson-Welsh L
    J. Exp. Med., 2012;209(7):1363-77.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Axl is essential for VEGF-A-dependent activation of PI3K/Akt.
    Authors: Ruan GX, Kazlauskas A,
    EMBO J., 2012;31(7):1692-703.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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