Human Siglec-14 Antibody Summary
Accession # Q08ET2
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Siglec-14 in HEK293 Human Cell Line Transfected with Human Siglec-14 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with Human Siglec-14 (filled histogram) or irrelevant protein (open histogram) were stained with Rabbit Anti-Human Siglec-14 Monoclonal Antibody (Catalog # MAB4905) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Gate was set based on Rabbit IgG isotype control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Siglecs are sialic acid specific I-type lectins that belong to the immunoglobulin superfamily. Structurally, they are transmembrane proteins with an N-terminal Ig-like V-set domain followed by a varying number of Ig-like C2-set domains (1, 2). Orthologs of Siglec-14 have not been described in mouse or rat. Mature human Siglec-14 consists of a 342 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and two Ig-like C2-set domains, a 23 aa transmembrane segment, and a 15 aa cytoplasmic tail (3). Within the first two Ig-like domains, Siglec-14 shares 99.5% aa sequence identity with Siglec-5, and 51% - 56% with Siglec-3, -6, -7, -8, and -9. Siglec-5 and -14 exhibit similar ligand preference among sialylated glycans, although Siglec-14 binds with higher avidity (3). Siglec-14 does not have the cytoplasmic ITIM sequence that mediates inhibitory signaling of most other Siglecs (2, 3). However, its transmembrane segment contains a charged arginine residue that enables association with the adaptor protein DAP12 (3, 4). Siglec-15 is the only other human Siglec identified to date that associates with DAP12 (5). Siglec-14 mRNA is primarily expressed in bone marrow, spleen, and fetal liver (3). The protein may be expressed on neutrophils, monocytes, and macrophages, as reports describing Siglec-5 on these cells employed some antibodies that also detect Siglec-14 (3, 6, 7). Siglec-5 and -14 likely function as paired receptors with similar ligand specificity and cellular expression but potentially opposing effects on cellular activation (3).
- Varki, A. and T. Angata (2006) Glycobiology 16:1R.
- Crocker, P.R. et al. (2007) Nat. Rev. Immunol. 7:255.
- Angata, T. et al. (2006) FASEB J. 20:1964.
- Turnbull, I.R. and M. Colonna (2007) Nat. Rev. Immunol. 7:155.
- Angata, T. et al. (2007) Glycobiology 17:838.
- Connolly, N.P. et al. (2002) Br. J. Haematol. 119:221.
- Erickson-Miller, C.L. et al. (2003) Exp. Hematol. 31:382.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human Siglec-14 Antibody
There are currently no reviews for this product. Be the first to review Human Siglec-14 Antibody and earn rewards!
Have you used Human Siglec-14 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image