Human Siglec-14 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB4905-100
MAB4905-SP
Detection of Siglec-14  in HEK293 Human Cell Line Transfected with Human Siglec-14 by Flow  Cytometry.
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Human Siglec-14 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Siglec-14 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2457E
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line, NS0-derived human Siglec-14
Lys18-Leu358
Accession # Q08ET2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Siglec-14  in HEK293 Human Cell Line Transfected with Human Siglec-14 by Flow  Cytometry. View Larger

Detection of Siglec-14 in HEK293 Human Cell Line Transfected with Human Siglec-14 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with Human Siglec-14 (filled histogram) or irrelevant protein (open histogram) were stained with Rabbit Anti-Human Siglec-14 Monoclonal Antibody (Catalog # MAB4905) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Gate was set based on Rabbit IgG isotype control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-14

Siglecs are sialic acid specific I-type lectins that belong to the immunoglobulin superfamily. Structurally, they are transmembrane proteins with an N-terminal Ig-like V-set domain followed by a varying number of Ig-like C2-set domains (1, 2). Orthologs of Siglec-14 have not been described in mouse or rat. Mature human Siglec-14 consists of a 342 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and two Ig-like C2-set domains, a 23 aa transmembrane segment, and a 15 aa cytoplasmic tail (3). Within the first two Ig-like domains, Siglec-14 shares 99.5% aa sequence identity with Siglec-5, and 51% - 56% with Siglec-3, -6, -7, -8, and -9. Siglec-5 and -14 exhibit similar ligand preference among sialylated glycans, although Siglec-14 binds with higher avidity (3). Siglec-14 does not have the cytoplasmic ITIM sequence that mediates inhibitory signaling of most other Siglecs (2, 3). However, its transmembrane segment contains a charged arginine residue that enables association with the adaptor protein DAP12 (3, 4). Siglec-15 is the only other human Siglec identified to date that associates with DAP12 (5). Siglec-14 mRNA is primarily expressed in bone marrow, spleen, and fetal liver (3). The protein may be expressed on neutrophils, monocytes, and macrophages, as reports describing Siglec-5 on these cells employed some antibodies that also detect Siglec-14 (3, 6, 7). Siglec-5 and -14 likely function as paired receptors with similar ligand specificity and cellular expression but potentially opposing effects on cellular activation (3).

References
  1. Varki, A. and T. Angata (2006) Glycobiology 16:1R.
  2. Crocker, P.R. et al. (2007) Nat. Rev. Immunol. 7:255.
  3. Angata, T. et al. (2006) FASEB J. 20:1964.
  4. Turnbull, I.R. and M. Colonna (2007) Nat. Rev. Immunol. 7:155.
  5. Angata, T. et al. (2007) Glycobiology 17:838.
  6. Connolly, N.P. et al. (2002) Br. J. Haematol. 119:221.
  7. Erickson-Miller, C.L. et al. (2003) Exp. Hematol. 31:382.
Long Name
Sialic Acid Binding Ig-like Lectin 14
Entrez Gene IDs
100049587 (Human)
Alternate Names
AC018755.2; sialic acid binding Ig-like lectin 14; Siglec14; Siglec-14

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