Human Siglec-15 Antibody

Catalog # Availability Size / Price Qty
Detection of Siglec-15 in HEK293 Human Cell Line transfected with Human Siglec-15 and eGFP by Flow Cytometry
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Product Details
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Human Siglec-15 Antibody Summary

Species Reactivity
Detects human Siglec-15 in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 1026113
Protein A or G purified from hybridoma culture supernatant
Chinese Hamster Ovary cell line, CHO-derived human Siglec-15
Accession # Q6ZMC9
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Flow Cytometry
0.25 µg/106 cells
HEK293 Human Cell Line transfected with Human Siglec-15 and eGFP
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Siglec-15 antibody in HEK293 Human Cell Line transfected with Human Siglec-15 and eGFP antibody by Flow Cytometry View Larger

Detection of Siglec-15 in HEK293 Human Cell Line transfected with Human Siglec-15 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with either (A) human Siglec-15 or (B) irrelevant protein, and eGFP, was stained with Mouse anti-human Siglec-15 monoclonal antibody (Catalog # MAB9227) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB002, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-15

Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Mature human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain (2). Within the ECD, human Siglec-15 shares 85% aa sequence identity with mouse and rat Siglec-15. Alternative splicing generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 associates with the signaling molecules DAP12 and DAP10 (2-5). It is expressed on osteoclasts, macrophages, and dendritic cells (2-6) and binds to the sialyl-Tn antigen (2, 3, 6). This interaction induces the production of TGF-beta by tumor-associated macrophages (3). Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts (4-6).

  1. Humphrey, M.B. and M.C. Nakamura (2015) Clin. Rev. Allergy Immunol. PMID 26573914.
  2. Angata, T. et al. (2007) Glycobiology 17:838.
  3. Takamiya, R. et al. (2013) Glycobiology 23:178.
  4. Kameda, Y. et al. (2013) J. Bone Miner. Res. 28:2463.
  5. Stuible, M. et al. (2014) J. Biol. Chem. 289:6498.
  6. Ishida-Kitagawa, N. et al. (2012) J. Biol. Chem. 287:17493.
Long Name
Sialic Acid Binding Ig-like Lectin 15
Entrez Gene IDs
284266 (Human); 620235 (Mouse); 498888 (Rat)
Alternate Names
CD33 antigen-like 3SIGLEC-15; CD33 molecule-like 3; CD33L3; HsT1361; sialic acid binding Ig-like lectin 15; sialic acid-binding Ig-like lectin 15; Siglec15; Siglec-15

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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