Human Siglec-9 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1139
Human Siglec-9 Biotinylated Antibody in Data
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Human Siglec-9 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Siglec‑9 in direct ELISAs and Western blots. In direct ELISAs, less than 0.2% cross-reactivity with recombinant human (rh) Siglec-2, rhSiglec-3, rhSiglec-5, rhSiglec-7, and rhSiglec-10 is observed. In Western blots, approximately 25% cross‑reactivity with rhSiglec-7 is observed, approximately 5% cross‑reactivity with rhSiglec-3 and rhSiglec-5 is observed, and less than 1% cross‑reactivity with rhSiglec-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec-9
Gln18-Gly348
Accession # Q9Y336
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Siglec‑9 Fc Chimera (Catalog # 1139-SL)
Flow Cytometry
0.25 µg/106 cells
See below
Immunocytochemistry
5-15 µg/mL
See below

Human Siglec-9 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human Siglec‑9 Antibody (Catalog # MAB1139)

Standard: Recombinant Human Siglec-9 Fc Chimera Protein, CF (Catalog # 1139-SL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Siglec‑9 antibody in Human Monocytes antibody by Flow Cytometry. View Larger

Detection of Siglec‑9 in Human Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Goat Anti-Human Siglec-9 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1139, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

Immunocytochemistry Siglec-9 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

Siglec‑9 in Human PBMCs. Siglec-9 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Siglec-9 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1139) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-9

Siglecs(1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglec-5 to -11 (1‑4). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (2, 3). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system.

The cDNA of human Siglec-9 encodes a 463 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-likeV-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (5, 6). In peripheral blood leukocytes, Siglec-9 is expressed on neutrophils, monocytes, a fraction of NK cells, B cells, and a minor subset of CD8+ T cells (5). It binds equally well to both 2,3- and 2,6-linked sialic acid (5, 6). Siglec-9 is closely related to Siglec-7, and they share ~80% amino acid sequence identity. The gene encoding siglec-9 was mapped to chromosome 19q13.4.

References
  1. Crocker, P.R. et al. (1998) Glycobiology 8:v.
  2. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
  3. Crocker, P.R. and A. Varki (2001) Immunology 103:137.
  4. Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
  5. Zhang, J.Q. et al. (2000) J. Biol. Chem. 275:22121.
  6. Angata, T. et al. (2000) J. Biol. Chem. 275:22127.
Long Name
Sialic Acid Binding Ig-like Lectin 9
Entrez Gene IDs
27180 (Human)
Alternate Names
CD329; CDw329; FOAP-9; OBBP-Like; Protein FOAP-9; sialic acid binding Ig-like lectin 9; sialic acid-binding Ig-like lectin 9; Siglec9; Siglec-9

Product Datasheets

Citation for Human Siglec-9 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Siglecs facilitate HIV-1 infection of macrophages through adhesion with viral sialic acids.
    Authors: Zou Z, Chastain A, Moir S
    PLoS ONE, 2011;6(9):e24559.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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