Human SLAM/CD150 Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot View Larger

Detection of Human SLAM/CD150 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SLAM/CD150 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF164) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SLAM/CD150 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Flow Cytometry View Larger

Detection of SLAM/CD150 in Human Lymphocytes by Flow Cytometry. Human whole blood lymphocytes were stained with Sheep Anti-Human SLAM/CD150 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF164, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

Preparation and Storage

Background: SLAM/CD150

SLAM (also IPO-3 and CD150) is a 70‑80 kDa member of the CD2 family of molecules. It is a costimulatory receptor that mediates T cell activation via SAP. SLAM is found on B cells, dendritic cells, macrophages, stem cells and Th1 T cells. SLAM engages in homophilic interactions. Mature human SLAM is 315 amino acids (aa) in length. It is a type I transmembrane glycoprotein whose extracellular domain contains one V-type and one C2-type Ig-like domain (aa 21‑237). There is a 77 aa SH2‑binding cytoplasmic domain. Four potential splice variants are suggested. One shows a deletion of aa 234‑263 (potentially soluble), while three others show aa substitutions; i.e.‑a ten aa substitution for aa 289‑335, a six aa substitution for aa 264‑335 and a 20 aa substitution for aa 125‑335 (potentially soluble). Over aa 1‑236, human SLAM shares 60% aa identity with mouse SLAM.

Product Datasheets

Related Research Areas

• B Cell Activation
• Co-stimulatory and Co-inhibitory Molecules
• Co-stimulatory Molecules Associated with Tfh Cells
• Dendritic Cell Co-stimulatory Molecules
• Dendritic Cell ITIM/ITAM Immunoreceptors
• Endothelial Cell Markers
• Hematopoietic Stem Cell Markers
• ITIM/ITAM Immunoreceptors and Related Molecules
• Lymphoma
• Macrophage Activation Markers
• Megakaryocytes
• Monocyte Markers
• Natural Killer T (NKT) Cells
• Platelet Immune Receptors
• Regulatory T Cells (Tregs)