Human SMAC/Diablo Antibody

(4 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human SMAC/Diablo in Western blots. Also detects mouse and rat SMAC/Diablo when the salt concentration of blotting buffer is reduced to 0.15 M NaCl, and 1 μg/mL anti-SMAC/Diablo concentration is used.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human SMAC/Diablo
    Ala56-Asp239
    Accession # Q9NR28
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human SMAC/Diablo by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, CHP-100 human neuroblastoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human SMAC/Diablo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF789) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human SMAC/Diablo (Catalog # 789-SM) (0.5 ng/lane) was included. A specific band for SMAC/Diablo was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
Detection of Human SMAC/Diablo by Simple WesternTM. Simple Western lane view shows lysates of SH‑SY5Y human neuroblastoma cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for SMAC/Diablo at approximately 29 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human SMAC/Diablo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF789). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SMAC/Diablo

SMAC (second mitochondria derived activator of caspase)/Diablo promotes caspase activation by interacting with the inhibitor of apoptosis (IAP) proteins in the cytochrome c/Apaf-1/caspase-9 pathway.

  • References:
    1. Du, C. et al. (2000) Cell 102:33.
    2. Chai, J. et al. (2000) Nature 406:855.
    3. Srinivasula, S. et al. (2000) J. Biol. Chem. 275:36152.
    4. Ekert, P. et al. (2001) J. Cell Biol. 152:483.
    5. Verhagen, A. (2000) Cell 102:43.
  • Long Name:
    Second Mitochondria-derived Activator of Caspase
  • Entrez Gene IDs:
    56616 (Human)
  • Alternate Names:
    0610041G12Rik; Diablo; diablo, IAP-binding mitochondrial protein; Direct IAP-binding protein with low pI; mitochondrial Smac protein; Second mitochondria-derived activator of caspase; SMAC; SMACmitochondrial
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Pro-Apoptotic Activity of New Honokiol/Triphenylmethane Analogues in B-Cell Lymphoid Malignancies
    Molecules, 2016;21(8):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Proteasome inhibition can impair caspase-8 activation upon submaximal stimulation of apoptotic tumor necrosis factor-related apoptosis inducing ligand (TRAIL) signaling.
    Authors: Laussmann MA, Passante E, Hellwig CT
    J. Biol. Chem., 2012;287(18):14402-11.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Inhibition of lymphotoxin-beta receptor-mediated cell death by survivin-DeltaEx3.
    Authors: You RI, Chou YC
    Cancer Res., 2006;66(6):3051-61.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. Role and regulation of nodal/activin receptor-like kinase 7 signaling pathway in the control of ovarian follicular atresia.
    Authors: Wang H, Jiang JY, Zhu C, Peng C, Tsang BK
    Mol. Endocrinol., 2006;20(10):2469-82.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 42  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Rabbit IgG Antibody

WB AF008 1
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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Average Rating: 5 (Based on 1 review)

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Western Blot Human SMAC/Diablo Antibody AF789
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 WB Mouse Anonymous 08/18/2017
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Western Blot Human SMAC/Diablo Antibody AF789
Western Blot: Human SMAC/Diablo Antibody [AF789]

Summary

ApplicationWestern Blot
Sample TestedRAW 264.7 mouse monocyte/macrophage cell line
SpeciesMouse

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