Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all SNAP29 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SNAP29
Ser2-Glu129
Accession # O95721

Specificity

Detects human SNAP29 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human SNAP29 Antibody

Detection of Human SNAP29 antibody by Western Blot.

Detection of Human SNAP29 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human SNAP29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7869) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SNAP29 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

SNAP29 antibody in K562 Human Cell Line by Immunocytochemistry (ICC).

SNAP29 in K562 Human Cell Line.

SNAP29 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Sheep Anti-Human SNAP29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7869) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Golgi granules in cytoplasm (STX6 staining shown in green, lower panel). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human SNAP29 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line

Western Blot

0.5 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and SK‑Mel‑28 human malignant melanoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: SNAP29

SNAP29 (29 kDa Soluble NSF [n-ethylmaleimide sensitive factor] Attachment Protein; also Synaptosomal associated protein 29 and GS32 [in rodent]) is a cytosolic and membrane-associated 27-32 kDa member of the syntaxin/ SNAP-25 family of proteins. It is widely, if not ubiquitously, expressed, and found in cell types as diverse as oligodentroglia, mast cells, neurons, Schwann cells and likely keratinocytes. Functionally, SNAP29 interacts with directly with syntaxin-1A and negatively impacts neurotransmission by inhibiting SNARE complex disassembly. In addition, it interacts with EHD1and AP-2, contributing to receptor-mediated endocytosis. Finally, it also might be said that SNAP29 is a key to the maintenance of general intracellular trafficking patterns. In this regard, SNAP29 has a remarkable ability to bind to a large number of syntaxins associated with multiple internal membranes. Human SNAP29 is 258 amino acids (aa) in length. It contains one t-SNARE coiled-coil homology domain (aa 196-258). Over aa 1-129, human and mouse SNAP29 share 88% aa sequence identity.

Long Name

Synaptosomal-associated Protein, 29 kDa

Alternate Names

CEDNIK

Entrez Gene IDs

9342 (Human); 67474 (Mouse); 116500 (Rat)

Gene Symbol

SNAP29

UniProt

O95721 (Human)

Additional SNAP29 Products

Product Documents for Human SNAP29 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human SNAP29 Antibody

For research use only

Citations for Human SNAP29 Antibody

Customer Reviews for Human SNAP29 Antibody

There are currently no reviews for this product. Be the first to review Human SNAP29 Antibody and earn rewards!

Have you used Human SNAP29 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies