Human SNAP29 Antibody

Catalog # Availability Size / Price Qty
AF7869
AF7869-SP
Human SNAP29 Antibody in Data
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Human SNAP29 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SNAP29 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SNAP29
Ser2-Glu129
Accession # O95721
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human SNAP29 antibody by Western Blot. View Larger

Detection of Human SNAP29 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human SNAP29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7869) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SNAP29 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry SNAP29 antibody in K562 Human Cell Line by Immunocytochemistry (ICC). View Larger

SNAP29 in K562 Human Cell Line. SNAP29 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Sheep Anti-Human SNAP29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7869) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Golgi granules in cytoplasm (STX6 staining shown in green, lower panel). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SNAP29

SNAP29 (29 kDa Soluble NSF [n-ethylmaleimide sensitive factor] Attachment Protein; also Synaptosomal associated protein 29 and GS32 [in rodent]) is a cytosolic and membrane-associated 27-32 kDa member of the syntaxin/ SNAP-25 family of proteins. It is widely, if not ubiquitously, expressed, and found in cell types as diverse as oligodentroglia, mast cells, neurons, Schwann cells and likely keratinocytes. Functionally, SNAP29 interacts with directly with syntaxin-1A and negatively impacts neurotransmission by inhibiting SNARE complex disassembly. In addition, it interacts with EHD1and AP-2, contributing to receptor-mediated endocytosis. Finally, it also might be said that SNAP29 is a key to the maintenance of general intracellular trafficking patterns. In this regard, SNAP29 has a remarkable ability to bind to a large number of syntaxins associated with multiple internal membranes. Human SNAP29 is 258 amino acids (aa) in length. It contains one t-SNARE coiled-coil homology domain (aa 196-258). Over aa 1-129, human and mouse SNAP29 share 88% aa sequence identity.

Long Name
Synaptosomal-associated Protein, 29 kDa
Entrez Gene IDs
9342 (Human); 67474 (Mouse); 116500 (Rat)
Alternate Names
CEDNIK; CEDNIKSoluble 29 kDa NSF attachment protein; SNAP29; SNAP-29FLJ21051; synaptosomal-associated protein 29; synaptosomal-associated protein, 29kD; synaptosomal-associated protein, 29kDa; Vesicle-membrane fusion protein SNAP-29

Product Datasheets

Citation for Human SNAP29 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. SNAP23 Regulates Endothelial Exocytosis of von Willebrand Factor.
    Authors: Zhu Q, Yamakuchi M, Lowenstein C
    PLoS ONE, 2015;10(8):e0118737.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human SNAP29 Antibody

There are currently no reviews for this product. Be the first to review Human SNAP29 Antibody and earn rewards!

Have you used Human SNAP29 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review