Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all SOX17 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SOX17
Asp177-Val414
Accession # Q9H6I2

Specificity

Detects human SOX17 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) SOX2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human SOX17 Biotinylated Antibody

SOX17 antibody in iBJ6 Cell Line by Immunocytochemistry (ICC).

SOX17 in iBJ6 Cell Line.

SOX17 was detected in immersion fixed iBJ6, an induced pluripotent stem (iPS) cell line, differentiated to endoderm using Goat Anti-Human SOX17 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1924) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Human SOX17 Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human BG01V embryonic stem cells and iBJ6 induced pluripotent stem cell line differentiated to endoderm

Western Blot

0.1 µg/mL
Sample: Recombinant Human SOX17

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SOX17

SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2). In Western blot, BAF1924 detects a 60 kDa band of endogenous SOX17 which is higher than predicted molecular weight but consistent with the current literature (3).

 

References

  1. Kanai-Azuma, M. et al. (2002) Development 129:2367.
  2. Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.
  3. Sohn, J. et al. (2006) J. Neuroscience 26:9722.

Long Name

Transcription Factor SOX17

Alternate Names

FLJ22252, SRY (sex determining region Y)-box 17, SRY-related HMG-box transcription factor SOX17, transcription factor SOX-17, VUR3

Entrez Gene IDs

64321 (Human)

Gene Symbol

SOX17

UniProt

Q9H6I2

Additional SOX17 Products

Product Documents for Human SOX17 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SOX17 Biotinylated Antibody

For research use only

Citations for Human SOX17 Biotinylated Antibody

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Protocols

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