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Human SOX17 Antibody

AF1924 106 Citations 2 Reviews Datasheet / COA / SDS
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AF1924
AF1924-SP
Detection of Human SOX17 by Western Blot.
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Product Details
Citations (106)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SOX17 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SOX17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SOX18 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SOX17
Asp177-Val414
Accession # Q9H6I2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human SOX17 by Western Blot. View Larger

Detection of Human SOX17 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOX17 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Chromatin Immunoprecipitation (ChIP) Detection of SOX17-regulated Genes by Chromatin Immunoprecipitation. View Larger

Detection of SOX17-regulated Genes by Chromatin Immunoprecipitation. Endoderm-differentiated D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX17/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The p21 promoter was detected by standard PCR.

Immunocytochemistry SOX17 in B16 Mouse Cell Line. View Larger

SOX17 in B16 Mouse Cell Line. SOX17 was detected in immersion fixed B16 mouse melanoma cell line using 10 µg/mL Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry SOX17 in Human BG01V Cells. View Larger

SOX17 in Human BG01V Cells. SOX17 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human SOX17 by Simple WesternTM. View Larger

Detection of Human SOX17 by Simple WesternTM. Simple Western lane view shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+), loaded at 0.2 mg/mL. A specific band was detected for SOX17 at approximately 59 kDa (as indicated) using 10 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western Detection of Human SOX17 by Simple WesternTM. View Larger

Detection of Human SOX17 by Simple WesternTM. Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or endoderm differentiated (+), loaded at 0.2 mg/mL. A specific band was detected for SOX17 at approximately 58 kDa (as indicated) using 10 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX17

SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).

References
  1. Kanai-Azuma, M. et al. (2002) Development 129:2367.
  2. Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.
Long Name
Transcription Factor SOX17
Entrez Gene IDs
64321 (Human)
Alternate Names
FLJ22252; SOX17; SRY (sex determining region Y)-box 17; SRY-related HMG-box transcription factor SOX17; transcription factor SOX-17; VUR3

Product Datasheets

Product Datasheet
COA
SDS

Citations for Human SOX17 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

106 Citations: Showing 1 - 10
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  1. A hPSC-based platform to discover gene-environment interactions that impact human ?-cell and dopamine neuron survival
    Authors: T Zhou, TW Kim, CN Chong, L Tan, S Amin, Z Sadat Badi, S Mukherjee, Z Ghazizadeh, H Zeng, M Guo, M Crespo, T Zhang, R Kenyon, CL Robinson, E Apostolou, H Wang, JZ Xiang, T Evans, L Studer, S Chen
    Nat Commun, 2018;9(1):4815.
    Species: Human
    Sample Types: Whole Cell
    Applications: ICC
  2. Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel
    Authors: X Gao, JJ Yourick, RL Sprando
    Stem Cell Res, 2018;31(0):193-196.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Establishment of STUB1/CHIP mutant induced pluripotent stem cells (iPSCs) from a patient with Gordon Holmes syndrome/SCAR16
    Authors: S Schuster, Y Schelling, M Synofzik, P Höflinger, L Schöls, S Hauser
    Stem Cell Res, 2018;29(0):166-169.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Induced pluripotent stem cells derived from a schizophrenia patient with ASTN2 deletion
    Authors: Y Arioka, I Kushima, H Kubo, D Mori, N Ozaki
    Stem Cell Res, 2018;30(0):81-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  5. Short-term retinoic acid treatment sustains pluripotency and suppresses differentiation of human induced pluripotent stem cells
    Authors: MT De Angelis, EI Parrotta, G Santamaria, G Cuda
    Cell Death Dis, 2018;9(1):6.
    Species: Human
    Sample Types: Embryoid Bodies
    Applications: ICC
  6. Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation
    Authors: NS Corsini, AM Peer, P Moeseneder, M Roiuk, TR Burkard, HC Theussl, I Moll, JA Knoblich
    Cell Stem Cell, 2018;22(4):543-558.e12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. G9a regulates temporal preimplantation developmental program and lineage segregation in blastocyst
    Authors: JJ Zylicz, M Borensztei, FC Wong, Y Huang, C Lee, S Dietmann, MA Surani
    Elife, 2018;7(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Generation of gene-corrected iPSC line from Parkinson's disease patient iPSC line with alpha-SNCA A53T mutation
    Authors: SY Lee, S Jeong, J Kim, SK Chung
    Stem Cell Res, 2018;30(0):145-149.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Generation of two induced pluripotent stem cell (iPSC) lines from p.F508del Cystic Fibrosis patients
    Authors: A Fleischer, IM Lorenzo, E Palomino, T Aasen, F Gómez, M Servera, VJ Asensio, V Gálvez, JC Izpisúa-Be, D Bachiller
    Stem Cell Res, 2018;29(0):1-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency
    Authors: K Adachi, W Kopp, G Wu, S Heising, B Greber, M Stehling, MJ Araúzo-Bra, ST Boerno, B Timmermann, M Vingron, HR Schöler
    Cell Stem Cell, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  11. Generation of a human Juvenile myelomonocytic leukemia iPSC line, CHOPi001-A, with a mutation in CBL
    Authors: AL Gagne, JA Maguire, S Gandre-Bab, ST Chou, SK Tasian, ML Loh, MJ Weiss, P Gadue, DL French
    Stem Cell Res, 2018;31(0):157-160.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Random Mutagenesis, Clonal Events, and Embryonic or Somatic Origin Determine the mtDNA Variant Type and Load in Human Pluripotent Stem�Cells
    Authors: F Zambelli, J Mertens, D Dziedzicka, J Sterckx, C Markouli, A Keller, P Tropel, L Jung, S Viville, H Van de Vel, M Geens, S Seneca, K Sermon, C Spits
    Stem Cell Reports, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Derivation of induced pluripotent stem cells in Japanese macaque (Macaca fuscata)
    Authors: R Nakai, M Ohnuki, K Kuroki, H Ito, H Hirai, R Kitajima, T Fujimoto, M Nakagawa, W Enard, M Imamura
    Sci Rep, 2018;8(1):12187.
    Species: Rhesus Macaque
    Sample Types: Whole Cells
    Applications: ICC
  14. Three lines of induced pluripotent stem cells derived from a 15q11.2-q13.1 duplication syndrome patient
    Authors: Y Arioka, I Kushima, D Mori, N Ozaki
    Stem Cell Res, 2018;31(0):240-243.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  15. yylncT Defines a Class of Divergently Transcribed lncRNAs and Safeguards the T-mediated Mesodermal Commitment of Human PSCs
    Authors: S Frank, G Ahuja, D Bartsch, N Russ, W Yao, JC Kuo, JP Derks, VS Akhade, Y Kargapolov, T Georgomano, JE Messling, M Gramm, L Brant, R Rehimi, NE Vargas, A Kuroczik, TP Yang, RGA Sahito, J Franzen, J Hescheler, A Sachinidis, M Peifer, A Rada-Igles, M Kanduri, IG Costa, C Kanduri, A Papantonis, L Kurian
    Cell Stem Cell, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  16. Generation of two induced pluripotent stem cells lines from Mucopolysaccharydosis IIIA patient: IMEDEAi004-A and IMEDEAi004-B
    Authors: S Vallejo, A Fleischer, JM Martín, A Sánchez, E Palomino, D Bachiller
    Stem Cell Res, 2018;32(0):110-114.
    Species: Human
    Sample Types:
  17. Generation of two induced pluripotent stem cells lines from a Mucopolysaccharydosis IIIB (MPSIIIB) patient
    Authors: S Vallejo-Di, A Fleischer, JM Martín-Fer, A Sánchez-Gi, D Bachiller
    Stem Cell Res, 2018;33(0):180-184.
    Species: Human
    Sample Types: Whole Cell
    Applications: ICC
  18. Derivation and characterization of the NIH registry human stem cell line NYSCF100 line under defined feeder-free conditions
    Authors: A Sevilla, E Forero, M Zimmer, H Martinez, K Reggio, D Paull, D Egli, S Noggle
    Stem Cell Res, 2018;29(0):99-102.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  19. Generation of an induced pluripotent stem cell line from a hypertrophic cardiomyopathy patient with a pathogenic myosin binding protein C (MYBPC3) p.Arg502Trp mutation
    Authors: M Holliday, SB Ross, S Lim, C Semsarian
    Stem Cell Res, 2018;33(0):56-59.
    Species: Human
    Sample Types: Whole Cell
    Applications: ICC
  20. Activation of the Aryl Hydrocarbon Receptor Interferes with Early Embryonic Development
    Authors: M Gialitakis, M Tolaini, Y Li, M Pardo, L Yu, A Toribio, JS Choudhary, K Niakan, V Papayannop, B Stockinger
    Stem Cell Reports, 2017;9(5):1377-1386.
    Species: Mouse
    Sample Types: Embryo
    Applications: IHC-Fr
  21. Peripheral blood derived induced pluripotent stem cells (iPSCs) from a female with familial hypertrophic cardiomyopathy
    Authors: SB Ross, ST Fraser, RD Bagnall, C Semsarian
    Stem Cell Res, 2017;20(0):76-79.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  22. Dynamics of 5-carboxylcytosine during hepatic differentiation: potential general role for active demethylation by DNA repair in lineage specification
    Authors: LC Lewis, PC Lo, JM Foster, N Dai, IR Corrêa, PM Durczak, G Duncan, A Ramsawhook, GP Aithal, C Denning, NR Hannan, A Ruzov
    Epigenetics, 2017;0(0):0.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. The chromatin modifier Satb1 regulates cell fate through Fgf signalling in the early mouse embryo
    Authors: M Goolam, M Zernicka-G
    Development, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. Acellular Mouse Kidney ECM can be Used as a Three-Dimensional Substrate to Test the Differentiation Potential of Embryonic Stem Cell Derived Renal Progenitors
    Authors: M Sambi, T Chow, J Whiteley, M Li, S Chua, V Raileanu, IM Rogers
    Stem Cell Rev, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Identification of a small molecule that facilitates the differentiation of human iPSCs/ESCs and mouse embryonic pancreatic explants into pancreatic endocrine cells
    Authors: Y Kondo, T Toyoda, R Ito, M Funato, Y Hosokawa, S Matsui, T Sudo, M Nakamura, C Okada, X Zhuang, A Watanabe, A Ohta, N Inagaki, K Osafune
    Diabetologia, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. Direct Reprogramming of Fibroblasts via a Chemically Induced XEN-like State
    Authors: X Li, D Liu, Y Ma, X Du, J Jing, L Wang, B Xie, D Sun, S Sun, X Jin, X Zhang, T Zhao, J Guan, Z Yi, W Lai, P Zheng, Z Huang, Y Chang, Z Chai, J Xu, H Deng
    Cell Stem Cell, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  27. Generation of induced pluripotent stem cells (iPSCs) from a hypertrophic cardiomyopathy patient with the pathogenic variant p.Val698Ala in beta-myosin heavy chain (MYH7) gene
    Authors: SB Ross, ST Fraser, N Nowak, C Semsarian
    Stem Cell Res, 2017;20(0):88-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  28. The circular RNA circBIRC6 participates in the molecular circuitry controlling human pluripotency
    Authors: CY Yu, TC Li, YY Wu, CH Yeh, W Chiang, CY Chuang, HC Kuo
    Nat Commun, 2017;8(1):1149.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  29. Insulin-producing cells derived from 'induced pluripotent stem cells' of patients with fulminant type 1 diabetes: vulnerability to cytokine insults and increased expression of apoptosis-related genes
    Authors: Y Hosokawa, T Toyoda, K Fukui, MY Baden, M Funato, Y Kondo, T Sudo, H Iwahashi, M Kishida, C Okada, A Watanabe, I Asaka, K Osafune, A Imagawa, I Shimomura
    J Diabetes Investig, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  30. Lineage Establishment and Progression within the Inner Cell Mass of the Mouse Blastocyst Requires FGFR1 and FGFR2
    Authors: M Kang, V Garg, AK Hadjantona
    Dev. Cell, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  31. Generation of a PXR reporter human induced pluripotent stem cell line (PXR-mCherry hiPSC) using the CRISPR/Cas9 system
    Authors: H Kim, JW Kim, SJ Kim, YJ Choi, DS Kim, HJ Park
    Stem Cell Res, 2017;26(0):72-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  32. Functional Thyroid Follicular Cells Differentiation from Human-Induced Pluripotent Stem Cells in Suspension Culture
    Authors: A Arauchi, K Matsuura, T Shimizu, T Okano
    Front Endocrinol (Lausanne), 2017;8(0):103.
    Species: Human
    Sample Types: Embryo Bodies
    Applications: Flow
  33. Rapid DNA replication origin licensing protects stem cell pluripotency
    Authors: JP Matson, R Dumitru, P Coryell, RM Baxley, W Chen, K Twaroski, BR Webber, J Tolar, AK Bielinsky, JE Purvis, JG Cook
    Elife, 2017;6(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  34. ICM conversion to epiblast by FGF/ERK inhibition is limited in time and requires transcription and protein degradation
    Authors: S Bessonnard, S Coqueran, S Vandormael, A Dufour, J Artus, M Cohen-Tann
    Sci Rep, 2017;7(1):12285.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  35. Embryonic cholecystitis and defective gallbladder contraction in the Sox17-haploinsufficient model of biliary atresia
    Authors: H Higashiyam, A Ozawa, H Sumitomo, M Uemura, K Fujino, H Igarashi, K Imaimatsu, N Tsunekawa, Y Hirate, M Kurohmaru, Y Saijoh, M Kanai-Azum, Y Kanai
    Development, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  36. Novel SOX17 frameshift mutations in endometrial cancer are functionally distinct from recurrent missense mutations
    Authors: CJ Walker, MJ O'Hern, VA Serna, T Kurita, MA Miranda, CE Sapp, DG Mutch, DE Cohn, PJ Goodfellow
    Oncotarget, 2017;8(40):68758-68768.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  37. Guided self-organization and cortical plate formation in human brain organoids
    Authors: MA Lancaster, NS Corsini, S Wolfinger, EH Gustafson, AW Phillips, TR Burkard, T Otani, FJ Livesey, JA Knoblich
    Nat. Biotechnol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  38. Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells
    Authors: F Sampazioti, MC de Brito, I Geti, A Bertero, NR Hannan, L Vallier
    Nat Protoc, 2017;12(4):814-827.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  39. Lymphoblast-derived integration-free ISRM-CON9 iPS cell line from a 75year old female
    Authors: S Martins, M Bohndorf, F Schröter, F Assar, W Wruck, K Sleegers, C Van Broeck, J Adjaye
    Stem Cell Res, 2017;26(0):76-79.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  40. Accreditation of Biosafe Clinical-Grade Human Embryonic Stem Cells According to Chinese Regulations
    Authors: Q Gu, J Wang, L Wang, ZX Liu, WW Zhu, YQ Tan, WF Han, J Wu, CJ Feng, JH Fang, L Liu, L Wang, W Li, XY Zhao, BY Hu, J Hao, Q Zhou
    Stem Cell Reports, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  41. TALEN-mediated shift of mitochondrial DNA heteroplasmy in MELAS-iPSCs with m.13513G>A mutation
    Authors: N Yahata, Y Matsumoto, M Omi, N Yamamoto, R Hata
    Sci Rep, 2017;7(1):15557.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  42. An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation
    Authors: J Saarimäki-, D Balboa, MA Russell, J Saarikettu, M Kinnunen, S Keskitalo, A Malhi, C Valensisi, C Andrus, S Eurola, H Grym, J Ustinov, K Wartiovaar, RD Hawkins, O Silvennoin, M Varjosalo, NG Morgan, T Otonkoski
    Cell Rep, 2017;19(2):281-294.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  43. Principles of early human development and germ cell program from conserved model systems
    Authors: T Kobayashi, H Zhang, WWC Tang, N Irie, S Withey, D Klisch, A Sybirna, S Dietmann, DA Contreras, R Webb, C Allegrucci, R Alberio, MA Surani
    Nature, 2017;546(7658):416-420.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  44. Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders
    Authors: C Lorenz, P Lesimple, R Bukowiecki, A Zink, G Inak, B Mlody, M Singh, M Semtner, N Mah, K Aur‚, M Leong, O Zabiegalov, EM Lyras, V Pfiffer, B Fauler, J Eichhorst, B Wiesner, N Huebner, J Priller, T Mielke, D Meierhofer, Z Izsv k, JC Meier, F Bouillaud, J Adjaye, M Schuelke, EE Wanker, A LombŠs, A Prigione
    Cell Stem Cell, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  45. Distinct Requirements for FGFR1 and FGFR2 in Primitive Endoderm Development and Exit from Pluripotency
    Authors: A Molotkov, P Mazot, JR Brewer, RM Cinalli, P Soriano
    Dev. Cell, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  46. Plasticity of the inner cell mass in mouse blastocyst is restricted by the activity of FGF/MAPK pathway
    Authors: M Wigger, K Kisielewsk, K Filimonow, B Plusa, M Maleszewsk, A Suwi?ska
    Sci Rep, 2017;7(1):15136.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  47. Xist-dependent imprinted X inactivation and the early developmental consequences of its failure
    Authors: M Borensztei, L Syx, K Ancelin, P Diabangoua, C Picard, T Liu, JB Liang, I Vassilev, R Galupa, N Servant, E Barillot, A Surani, CJ Chen, E Heard
    Nat. Struct. Mol. Biol, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  48. Inappropriate cadherin switching in the mouse epiblast compromises proper signaling between the epiblast and the extraembryonic ectoderm during gastrulation
    Sci Rep, 2016;6(0):26562.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC Not Specified
  49. Generation of optic atrophy 1 patient-derived induced pluripotent stem cells (iPS-OPA1-BEHR) for disease modeling of complex optic atrophy syndromes (Behr syndrome)
    Stem Cell Res, 2016;17(2):426-429.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC Fresh
  50. The Anterior-Posterior Patterning of Definitive Endoderm Generated from Human Embryonic Stem Cells Depends on the Differential Signaling of Retinoic Acid, Wnt- and BMP-Signaling
    Authors: Claudia Davenport
    Stem Cells, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC - PFA fixed
  51. Ductular reaction-on-a-chip: Microfluidic co-cultures to study stem cell fate selection during liver injury
    Sci Rep, 2016;6(0):36077.
    Species: Rat
    Sample Types: Whole Cells
    Applications: IHC
  52. Capturing the biology of disease severity in a PSC-based model of familial dysautonomia
    Authors: N Zeltner, F Fattahi, NC Dubois, N Saurat, F Lafaille, L Shang, B Zimmer, J Tchieu, MA Soliman, G Lee, JL Casanova, L Studer
    Nat. Med., 2016;22(12):1421-1427.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  53. Hepatic differentiation of human pluripotent stem cells in miniaturized format suitable for high-throughput screen
    Authors: A Carpentier, I Nimgaonkar, V Chu, Y Xia, Z Hu, TJ Liang
    Stem Cell Res, 2016;16(3):640-650.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometery
  54. Diverse Functions of Retinoic Acid in Brain Vascular Development
    J Neurosci, 2016;36(29):7786-801.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  55. p38 (Mapk14/11) occupies a regulatory node governing entry into primitive endoderm differentiation during preimplantation mouse embryo development
    Open Biol, 2016;6(9):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fluorescent
  56. Derivation of Patient Specific Pluripotent Stem Cells Using Clinically Discarded Cumulus Cells
    PLoS ONE, 2016;11(11):e0165715.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC - Paraffin embedded
  57. Changes in Parthenogenetic Imprinting Patterns during Reprogramming by Cell Fusion
    Authors: Hyun Sik Jang
    PLoS ONE, 2016;11(5):e0156491.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC - Not specified
  58. Efficient derivation of extraembryonic endoderm stem cell lines from mouse postimplantation embryos
    Sci Rep, 2016;6(0):39457.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  59. Selection and dynamics of embryonic stem cell integration into early mouse embryos.
    Authors: Alexandrova S, Kalkan T, Humphreys P, Riddell A, Scognamiglio R, Trumpp A, Nichols J
    Development, 2016;143(1):24-34.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Not specified
  60. HEB associates with PRC2 and SMAD2/3 to regulate developmental fates.
    Authors: Yoon, Se-Jin, Foley, Joseph W, Baker, Julie C
    Nat Commun, 2015;6(0):6546.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  61. Activin-A and Bmp4 levels modulate cell type specification during CHIR-induced cardiomyogenesis.
    Authors: Kim M, Horst A, Blinka S, Stamm K, Mahnke D, Schuman J, Gundry R, Tomita-Mitchell A, Lough J
    PLoS ONE, 2015;10(2):e0118670.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  62. Transcription activator-like effector nuclease (TALEN)-mediated CLYBL targeting enables enhanced transgene expression and one-step generation of dual reporter human induced pluripotent stem cell (iPSC) and neural stem cell (NSC) lines.
    Authors: Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
    PLoS ONE, 2015;10(1):e0116032.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC - Not specified
  63. Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells.
    Authors: Paull D, Sevilla A, Zhou H, Hahn A, Kim H, Napolitano C, Tsankov A, Shang L, Krumholz K, Jagadeesan P, Woodard C, Sun B, Vilboux T, Zimmer M, Forero E, Moroziewicz D, Martinez H, Malicdan M, Weiss K, Vensand L, Dusenberry C, Polus H, Sy K, Kahler D, Gahl W, Solomon S, Chang S, Meissner A, Eggan K, Noggle S
    Nat Methods, 2015;12(9):885-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC Not Specified
  64. Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis.
    Authors: Takasato M, Er P, Chiu H, Maier B, Baillie G, Ferguson C, Parton R, Wolvetang E, Roost M, Chuva de Sousa Lopes S, Little M
    Nature, 2015;526(7574):564-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Not Specified
  65. Design of a Vitronectin-Based Recombinant Protein as a Defined Substrate for Differentiation of Human Pluripotent Stem Cells into Hepatocyte-Like Cells.
    Authors: Nagaoka M, Kobayashi M, Kawai C, Mallanna S, Duncan S
    PLoS ONE, 2015;10(8):e0136350.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC - Not specified
  66. Citrullination regulates pluripotency and histone H1 binding to chromatin.
    Authors: Christophorou M, Castelo-Branco G, Halley-Stott R, Oliveira C, Loos R, Radzisheuskaya A, Mowen K, Bertone P, Silva J, Zernicka-Goetz M, Nielsen M, Gurdon J, Kouzarides T
    Nature, 2014;507(7490):104-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Not Specified
  67. Discovery of directional and nondirectional pioneer transcription factors by modeling DNase profile magnitude and shape.
    Authors: Sherwood, Richard, Hashimoto, Tatsunor, O'Donnell, Charles, Lewis, Sophia, Barkal, Amira A, van Hoff, John Pet, Karun, Vivek, Jaakkola, Tommi, Gifford, David K
    Nat Biotechnol, 2014;32(2):171-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  68. Derivation of transgene-free human induced pluripotent stem cells from human peripheral T cells in defined culture conditions.
    Authors: Kishino, Yoshikaz, Seki, Tomohisa, Fujita, Jun, Yuasa, Shinsuke, Tohyama, Shugo, Kunitomi, Akira, Tabei, Ryota, Nakajima, Kazuaki, Okada, Marina, Hirano, Akinori, Kanazawa, Hideaki, Fukuda, Keiichi
    PLoS ONE, 2014;9(5):e97397.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  69. A modified EpiSC culture condition containing a GSK3 inhibitor can support germline-competent pluripotency in mice.
    Authors: Tsukiyama, Tomoyuki, Ohinata, Yasuhide
    PLoS ONE, 2014;9(4):e95329.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  70. Efficient programming of human eye conjunctiva-derived induced pluripotent stem (ECiPS) cells into definitive endoderm-like cells.
    Authors: Massumi M, Hoveizi E, Baktash P, Hooti A, Ghazizadeh L, Nadri S, Pourasgari F, Hajarizadeh A, Soleimani M, Nabiuni M, Khorramizadeh M
    Exp Cell Res, 2014;322(1):51-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  71. Sox17-mediated maintenance of fetal intra-aortic hematopoietic cell clusters.
    Authors: Nobuhisa I, Osawa M, Uemura M, Kishikawa Y, Anani M, Harada K, Takagi H, Saito K, Kanai-Azuma M, Kanai Y, Iwama A, Taga T
    Mol Cell Biol, 2014;34(11):1976-90.
    Species: Mouse
    Sample Types: Embryo
    Applications: IHC OCT-embedded
  72. GATA6 levels modulate primitive endoderm cell fate choice and timing in the mouse blastocyst.
    Authors: Schrode N, Saiz N, Di Talia S, Hadjantonakis A
    Dev Cell, 2014;29(4):454-67.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  73. Unfolded protein response is required for the definitive endodermal specification of mouse embryonic stem cells via Smad2 and beta-catenin signaling.
    Authors: Xu H, Tsang K, Wang Y, Chan J, Xu G, Gao W
    J Biol Chem, 2014;289(38):26290-301.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC Not Specified
  74. Induction of pluripotency in human somatic cells via a transient state resembling primitive streak-like mesendoderm.
    Authors: Takahashi K, Tanabe K, Ohnuki M, Narita M, Sasaki A, Yamamoto M, Nakamura M, Sutou K, Osafune K, Yamanaka S
    Nat Commun, 2014;5(0):3678.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC - PFA fixed
  75. High oxygen condition facilitates the differentiation of mouse and human pluripotent stem cells into pancreatic progenitors and insulin-producing cells.
    Authors: Hakim F, Kaitsuka T, Raeed J, Wei F, Shiraki N, Akagi T, Yokota T, Kume S, Tomizawa K
    J Biol Chem, 2014;289(14):9623-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  76. Generation and characterization of induced pluripotent stem cells from Aid-deficient mice.
    Authors: Shimamoto R, Amano N, Ichisaka T, Watanabe A, Yamanaka S, Okita K
    PLoS ONE, 2014;9(4):e94735.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  77. miR-373 is regulated by TGFbeta signaling and promotes mesendoderm differentiation in human Embryonic Stem Cells.
    Authors: Rosa A, Papaioannou M, Krzyspiak J, Brivanlou A
    Dev Biol, 2014;391(1):81-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  78. BMP signalling regulates the pre-implantation development of extra-embryonic cell lineages in the mouse embryo.
    Authors: Graham, Sarah J, Wicher, Krzyszto, Jedrusik, Agnieszk, Guo, Guoji, Herath, Wishva, Robson, Paul, Zernicka-Goetz, Magdalen
    Nat Commun, 2014;5(0):5667.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  79. Generation of functional insulin-producing cells from mouse embryonic stem cells through 804G cell-derived extracellular matrix and protein transduction of transcription factors.
    Authors: Kaitsuka T, Noguchi H, Shiraki N, Kubo T, Wei F, Hakim F, Kume S, Tomizawa K
    Stem Cells Transl Med, 2014;3(1):114-27.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  80. Production of hepatocyte-like cells from human pluripotent stem cells.
    Authors: Hannan, Nicholas, Segeritz, Charis-P, Touboul, Thomas, Vallier, Ludovic
    Nat Protoc, 2013;8(2):430-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  81. Cell-cycle control of developmentally regulated transcription factors accounts for heterogeneity in human pluripotent cells.
    Authors: Singh A, Chappell J, Trost R, Lin L, Wang T, Tang J, Matlock B, Weller K, Wu H, Zhao S, Jin P, Dalton S
    Stem Cell Reports, 2013;1(6):532-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  82. Identification of transcription factors for lineage-specific ESC differentiation.
    Authors: Yamamizu K, Piao Y, Sharov A, Zsiros V, Yu H, Nakazawa K, Schlessinger D, Ko M
    Stem Cell Reports, 2013;1(6):545-59.
  83. Defined extracellular matrix components are necessary for definitive endoderm induction.
    Authors: Taylor-Weiner H, Schwarzbauer J, Engler A
    Stem Cells, 2013;31(10):2084-94.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  84. Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme.
    Authors: Sneddon, Julie B, Borowiak, Malgorza, Melton, Douglas
    Nature, 2012;491(7426):765-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC Fluorescence
  85. The endothelial cell line bEnd.3 maintains human pluripotent stem cells.
    Authors: Joubin, Katherin, Richardson, Amelia, Novoa, Natalia, Tu, Edmund, Tomishima, Mark J
    Stem Cells Dev, 2012;21(12):2312-21.
    Species: Human
    Sample Types: Whole Cells
  86. CD166(pos) subpopulation from differentiated human ES and iPS cells support repair of acute lung injury.
    Authors: Soh, Boon Sen, Zheng, Dahai, Li Yeo, Julie Su, Yang, Henry He, Ng, Shi Yan, Wong, Lan Hion, Zhang, Wencai, Li, Pin, Nichane, Massimo, Asmat, Atasha, Wong, Poo Sing, Wong, Peng Che, Su, Lin Lin, Mantalaris, Sakis A, Lu, Jia, Xian, Wa, McKeon, Frank, Chen, Jianzhu, Lim, Elaine H, Lim, Bing
    Mol Ther, 2012;20(12):2335-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  87. A molecular signature for purified definitive endoderm guides differentiation and isolation of endoderm from mouse and human embryonic stem cells.
    Authors: Wang P, McKnight K, Wong D, Rodriguez R, Sugiyama T, Gu X, Ghodasara A, Qu K, Chang H, Kim S
    Stem Cells Dev, 2012;21(12):2273-87.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  88. A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3.
    Authors: Bone HK, Nelson AS, Goldring CE, Tosh D, Welham MJ
    J. Cell. Sci., 2011;124(0):1992-2000.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  89. Early in vitro differentiation of mouse definitive endoderm is not correlated with progressive maturation of nuclear DNA methylation patterns.
    Authors: Tajbakhsh J, Gertych A, Fagg WS
    PLoS ONE, 2011;6(7):e21861.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  90. CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived from human embryonic stem cells.
    Authors: Jiang W, Sui X, Zhang D, Liu M, Ding M, Shi Y, Deng H
    Stem Cells, 2011;29(4):609-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  91. Alginate microcapsule for propagation and directed differentiation of hESCs to definitive endoderm.
    Authors: Chayosumrit M, Tuch B, Sidhu K
    Biomaterials, 2010;31(3):505-14.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  92. Induction of pluripotent stem cells from human third molar mesenchymal stromal cells.
    Authors: Oda Y, Yoshimura Y, Ohnishi H
    J. Biol. Chem., 2010;285(38):29270-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  93. Origin and formation of the first two distinct cell types of the inner cell mass in the mouse embryo.
    Authors: Morris SA, Teo RT, Li H, Robson P, Glover DM, Zernicka-Goetz M
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(14):6364-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  94. A role for PDGF signaling in expansion of the extra-embryonic endoderm lineage of the mouse blastocyst.
    Authors: Artus J, Panthier JJ, Hadjantonakis AK
    Development, 2010;137(20):3361-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  95. SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cells.
    Authors: Chng Z, Teo A, Pedersen RA, Vallier L
    Cell Stem Cell, 2010;6(1):59-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  96. The ROCK inhibitor Y-27632 improves recovery of human embryonic stem cells after fluorescence-activated cell sorting with multiple cell surface markers.
    Authors: Emre N, Vidal JG, Elia J
    PLoS ONE, 2010;5(8):e12148.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  97. A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells.
    Authors: Hansson M, Olesen DR, Peterslund JM, Engberg N, Kahn M, Winzi M, Klein T, Maddox-Hyttel P, Serup P
    Dev. Biol., 2009;330(2):286-304.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  98. Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
    Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH
    FEBS Lett., 2009;583(3):561-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  99. Biphasic induction of Pdx1 in mouse and human embryonic stem cells can mimic development of pancreatic beta-cells.
    Authors: Bernardo AS, Cho CH, Mason S, Docherty HM, Pedersen RA, Vallier L, Docherty K
    Stem Cells, 2009;27(2):341-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  100. Highly efficient differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells.
    Authors: Zhang D, Jiang W, Liu M, Sui X, Yin X, Chen S, Shi Y, Deng H
    Cell Res., 2009;19(4):429-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  101. Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells.
    Authors: Sun N, Panetta NJ, Gupta DM, Wilson KD, Lee A, Jia F, Hu S, Cherry AM, Robbins RC, Longaker MT, Wu JC
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(37):15720-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  102. Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells.
    Authors: Borowiak M, Maehr R, Chen S, Chen AE, Tang W, Fox JL, Schreiber SL, Melton DA
    Cell Stem Cell, 2009;4(4):348-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  103. High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells.
    Authors: Desbordes SC, Placantonakis DG, Ciro A, Socci ND, Lee G, Djaballah H, Studer L
    Cell Stem Cell, 2008;2(6):602-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  104. Prospective isolation and global gene expression analysis of definitive and visceral endoderm.
    Authors: Sherwood RI, Jitianu C, Cleaver O, Shaywitz DA, Lamenzo JO, Chen AE, Golub TR, Melton DA
    Dev. Biol., 2007;304(2):541-55.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  105. Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions.
    Authors: Yao S, Chen S, Clark J, Hao E, Beattie GM, Hayek A, Ding S
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(18):6907-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  106. Generation of a human Ocular Albinism type 1 iPSC line, SEIi001-A, with a mutation in GPR143.
    Authors: Baulier E, Garcia Diaz A, Corneo B, Farber D
    Stem Cell Res, 0;33(0):274-277.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Human SOX17 Antibody
By Anonymous on 04/13/2018
Application: WB Sample Tested: Tumor cell lyastes Species: Human
Human SOX17 Antibody
By Anonymous on 12/15/2016
Application: ICC/IF Sample Tested: Induced Pluripotent Stem Cells,Endoderm Species: Human

Used this antibody to verify the endodermal germ layer derived from iPSC.