Human SOX17 Antibody

(69 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human SOX17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SOX18 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human SOX17
    Asp177-Val414
    Accession # Q9H6I2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Chromatin Immunoprecipitation (ChIP)
    5 µg/5 x 106 cells
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human SOX17 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOX17 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chromatin Immunoprecipitation (ChIP)
Detection of SOX17-regulated Genes by Chromatin Immunoprecipitation. Endoderm-differentiated D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX17/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The p21 promoter was detected by standard PCR.
Immunocytochemistry
SOX17 in B16 Mouse Cell Line. SOX17 was detected in immersion fixed B16 mouse melanoma cell line using 10 µg/mL Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
SOX17 in Human BG01V Cells. SOX17 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human SOX17 by Simple WesternTM. Simple Western lane view shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+), loaded at 0.2 mg/mL. A specific band was detected for SOX17 at approximately 59 kDa (as indicated) using 10 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human SOX17 by Simple WesternTM. Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or endoderm differentiated (+), loaded at 0.2 mg/mL. A specific band was detected for SOX17 at approximately 58 kDa (as indicated) using 10 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX17

SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).

  • References:
    1. Kanai-Azuma, M. et al. (2002) Development 129:2367.
    2. Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.
  • Long Name:
    Transcription Factor SOX17
  • Entrez Gene IDs:
    64321 (Human)
  • Alternate Names:
    FLJ22252; SOX17; SRY (sex determining region Y)-box 17; SRY-related HMG-box transcription factor SOX17; transcription factor SOX-17; VUR3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

69 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Guided self-organization and cortical plate formation in human brain organoids
    Authors: MA Lancaster, NS Corsini, S Wolfinger, EH Gustafson, AW Phillips, TR Burkard, T Otani, FJ Livesey, JA Knoblich
    Nat. Biotechnol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  2. Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells
    Authors: F Sampazioti, MC de Brito, I Geti, A Bertero, NR Hannan, L Vallier
    Nat Protoc, 2017;12(4):814-827.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  3. Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders
    Authors: C Lorenz, P Lesimple, R Bukowiecki, A Zink, G Inak, B Mlody, M Singh, M Semtner, N Mah, K Aur‚, M Leong, O Zabiegalov, EM Lyras, V Pfiffer, B Fauler, J Eichhorst, B Wiesner, N Huebner, J Priller, T Mielke, D Meierhofer, Z Izsv k, JC Meier, F Bouillaud, J Adjaye, M Schuelke, EE Wanker, A LombŠs, A Prigione
    Cell Stem Cell, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. Xist-dependent imprinted X inactivation and the early developmental consequences of its failure
    Authors: M Borensztei, L Syx, K Ancelin, P Diabangoua, C Picard, T Liu, JB Liang, I Vassilev, R Galupa, N Servant, E Barillot, A Surani, CJ Chen, E Heard
    Nat. Struct. Mol. Biol, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. Peripheral blood derived induced pluripotent stem cells (iPSCs) from a female with familial hypertrophic cardiomyopathy
    Authors: SB Ross, ST Fraser, RD Bagnall, C Semsarian
    Stem Cell Res, 2017;20(0):76-79.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  6. Generation of induced pluripotent stem cells (iPSCs) from a hypertrophic cardiomyopathy patient with the pathogenic variant p.Val698Ala in beta-myosin heavy chain (MYH7) gene
    Authors: SB Ross, ST Fraser, N Nowak, C Semsarian
    Stem Cell Res, 2017;20(0):88-90.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  7. An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation
    Authors: J Saarimäki-, D Balboa, MA Russell, J Saarikettu, M Kinnunen, S Keskitalo, A Malhi, C Valensisi, C Andrus, S Eurola, H Grym, J Ustinov, K Wartiovaar, RD Hawkins, O Silvennoin, M Varjosalo, NG Morgan, T Otonkoski
    Cell Rep, 2017;19(2):281-294.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. Insulin-producing cells derived from 'induced pluripotent stem cells' of patients with fulminant type 1 diabetes: vulnerability to cytokine insults and increased expression of apoptosis-related genes
    Authors: Y Hosokawa, T Toyoda, K Fukui, MY Baden, M Funato, Y Kondo, T Sudo, H Iwahashi, M Kishida, C Okada, A Watanabe, I Asaka, K Osafune, A Imagawa, I Shimomura
    J Diabetes Investig, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  9. Accreditation of Biosafe Clinical-Grade Human Embryonic Stem Cells According to Chinese Regulations
    Authors: Q Gu, J Wang, L Wang, ZX Liu, WW Zhu, YQ Tan, WF Han, J Wu, CJ Feng, JH Fang, L Liu, L Wang, W Li, XY Zhao, BY Hu, J Hao, Q Zhou
    Stem Cell Reports, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  10. Principles of early human development and germ cell program from conserved model systems
    Authors: T Kobayashi, H Zhang, WWC Tang, N Irie, S Withey, D Klisch, A Sybirna, S Dietmann, DA Contreras, R Webb, C Allegrucci, R Alberio, MA Surani
    Nature, 2017;546(7658):416-420.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  11. Direct Reprogramming of Fibroblasts via a Chemically Induced XEN-like State
    Authors: X Li, D Liu, Y Ma, X Du, J Jing, L Wang, B Xie, D Sun, S Sun, X Jin, X Zhang, T Zhao, J Guan, Z Yi, W Lai, P Zheng, Z Huang, Y Chang, Z Chai, J Xu, H Deng
    Cell Stem Cell, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  12. Diverse Functions of Retinoic Acid in Brain Vascular Development
    J Neurosci, 2016;36(29):7786-801.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  13. Selection and dynamics of embryonic stem cell integration into early mouse embryos.
    Authors: Alexandrova S, Kalkan T, Humphreys P, Riddell A, Scognamiglio R, Trumpp A, Nichols J
    Development, 2016;143(1):24-34.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  14. The Anterior-Posterior Patterning of Definitive Endoderm Generated from Human Embryonic Stem Cells Depends on the Differential Signaling of Retinoic Acid, Wnt- and BMP-Signaling
    Authors: Claudia Davenport
    Stem Cells, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC - PFA fixed
  15. Changes in Parthenogenetic Imprinting Patterns during Reprogramming by Cell Fusion
    Authors: Hyun Sik Jang
    PLoS ONE, 2016;11(5):e0156491.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC - Not specified
  16. Hepatic differentiation of human pluripotent stem cells in miniaturized format suitable for high-throughput screen
    Authors: A Carpentier, I Nimgaonkar, V Chu, Y Xia, Z Hu, TJ Liang
    Stem Cell Res, 2016;16(3):640-650.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow Cytometery
  17. Inappropriate cadherin switching in the mouse epiblast compromises proper signaling between the epiblast and the extraembryonic ectoderm during gastrulation
    Sci Rep, 2016;6(0):26562.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Not Specified
  18. Efficient derivation of extraembryonic endoderm stem cell lines from mouse postimplantation embryos
    Sci Rep, 2016;6(0):39457.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  19. p38 (Mapk14/11) occupies a regulatory node governing entry into primitive endoderm differentiation during preimplantation mouse embryo development
    Open Biol, 2016;6(9):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-Fluorescent
  20. Generation of optic atrophy 1 patient-derived induced pluripotent stem cells (iPS-OPA1-BEHR) for disease modeling of complex optic atrophy syndromes (Behr syndrome)
    Stem Cell Res, 2016;17(2):426-429.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Fresh
  21. Ductular reaction-on-a-chip: Microfluidic co-cultures to study stem cell fate selection during liver injury
    Sci Rep, 2016;6(0):36077.
    Species: Rat
    Sample Type: Whole Cells
    Application: IHC
  22. Derivation of Patient Specific Pluripotent Stem Cells Using Clinically Discarded Cumulus Cells
    PLoS ONE, 2016;11(11):e0165715.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC - Paraffin embedded
  23. Capturing the biology of disease severity in a PSC-based model of familial dysautonomia
    Authors: N Zeltner, F Fattahi, NC Dubois, N Saurat, F Lafaille, L Shang, B Zimmer, J Tchieu, MA Soliman, G Lee, JL Casanova, L Studer
    Nat. Med., 2016;22(12):1421-1427.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  24. HEB associates with PRC2 and SMAD2/3 to regulate developmental fates.
    Authors: Yoon, Se-Jin, Foley, Joseph W, Baker, Julie C
    Nat Commun, 2015;6(0):6546.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  25. Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells.
    Authors: Paull D, Sevilla A, Zhou H, Hahn A, Kim H, Napolitano C, Tsankov A, Shang L, Krumholz K, Jagadeesan P, Woodard C, Sun B, Vilboux T, Zimmer M, Forero E, Moroziewicz D, Martinez H, Malicdan M, Weiss K, Vensand L, Dusenberry C, Polus H, Sy K, Kahler D, Gahl W, Solomon S, Chang S, Meissner A, Eggan K, Noggle S
    Nat Methods, 2015;12(9):885-92.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Not Specified
  26. Design of a Vitronectin-Based Recombinant Protein as a Defined Substrate for Differentiation of Human Pluripotent Stem Cells into Hepatocyte-Like Cells.
    Authors: Nagaoka M, Kobayashi M, Kawai C, Mallanna S, Duncan S
    PLoS ONE, 2015;10(8):e0136350.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC - Not specified
  27. Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis.
    Authors: Takasato M, Er P, Chiu H, Maier B, Baillie G, Ferguson C, Parton R, Wolvetang E, Roost M, Chuva de Sousa Lopes S, Little M
    Nature, 2015;526(7574):564-8.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  28. Transcription activator-like effector nuclease (TALEN)-mediated CLYBL targeting enables enhanced transgene expression and one-step generation of dual reporter human induced pluripotent stem cell (iPSC) and neural stem cell (NSC) lines.
    Authors: Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
    PLoS ONE, 2015;10(1):e0116032.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC - Not specified
  29. Activin-A and Bmp4 levels modulate cell type specification during CHIR-induced cardiomyogenesis.
    Authors: Kim M, Horst A, Blinka S, Stamm K, Mahnke D, Schuman J, Gundry R, Tomita-Mitchell A, Lough J
    PLoS ONE, 2015;10(2):e0118670.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  30. miR-373 is regulated by TGFbeta signaling and promotes mesendoderm differentiation in human Embryonic Stem Cells.
    Authors: Rosa A, Papaioannou M, Krzyspiak J, Brivanlou A
    Dev Biol, 2014;391(1):81-8.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  31. Induction of pluripotency in human somatic cells via a transient state resembling primitive streak-like mesendoderm.
    Authors: Takahashi K, Tanabe K, Ohnuki M, Narita M, Sasaki A, Yamamoto M, Nakamura M, Sutou K, Osafune K, Yamanaka S
    Nat Commun, 2014;5(0):3678.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC - PFA fixed
  32. BMP signalling regulates the pre-implantation development of extra-embryonic cell lineages in the mouse embryo.
    Authors: Graham, Sarah J, Wicher, Krzyszto, Jedrusik, Agnieszk, Guo, Guoji, Herath, Wishva, Robson, Paul, Zernicka-Goetz, Magdalen
    Nat Commun, 2014;5(0):5667.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  33. Efficient programming of human eye conjunctiva-derived induced pluripotent stem (ECiPS) cells into definitive endoderm-like cells.
    Authors: Massumi M, Hoveizi E, Baktash P, Hooti A, Ghazizadeh L, Nadri S, Pourasgari F, Hajarizadeh A, Soleimani M, Nabiuni M, Khorramizadeh M
    Exp Cell Res, 2014;322(1):51-61.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  34. Discovery of directional and nondirectional pioneer transcription factors by modeling DNase profile magnitude and shape.
    Authors: Sherwood, Richard, Hashimoto, Tatsunor, O'Donnell, Charles, Lewis, Sophia, Barkal, Amira A, van Hoff, John Pet, Karun, Vivek, Jaakkola, Tommi, Gifford, David K
    Nat Biotechnol, 2014;32(2):171-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  35. Citrullination regulates pluripotency and histone H1 binding to chromatin.
    Authors: Christophorou M, Castelo-Branco G, Halley-Stott R, Oliveira C, Loos R, Radzisheuskaya A, Mowen K, Bertone P, Silva J, Zernicka-Goetz M, Nielsen M, Gurdon J, Kouzarides T
    Nature, 2014;507(7490):104-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  36. Generation of functional insulin-producing cells from mouse embryonic stem cells through 804G cell-derived extracellular matrix and protein transduction of transcription factors.
    Authors: Kaitsuka T, Noguchi H, Shiraki N, Kubo T, Wei F, Hakim F, Kume S, Tomizawa K
    Stem Cells Transl Med, 2014;3(1):114-27.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  37. GATA6 levels modulate primitive endoderm cell fate choice and timing in the mouse blastocyst.
    Authors: Schrode N, Saiz N, Di Talia S, Hadjantonakis A
    Dev Cell, 2014;29(4):454-67.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  38. Derivation of transgene-free human induced pluripotent stem cells from human peripheral T cells in defined culture conditions.
    Authors: Kishino, Yoshikaz, Seki, Tomohisa, Fujita, Jun, Yuasa, Shinsuke, Tohyama, Shugo, Kunitomi, Akira, Tabei, Ryota, Nakajima, Kazuaki, Okada, Marina, Hirano, Akinori, Kanazawa, Hideaki, Fukuda, Keiichi
    PLoS ONE, 2014;9(5):e97397.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  39. High oxygen condition facilitates the differentiation of mouse and human pluripotent stem cells into pancreatic progenitors and insulin-producing cells.
    Authors: Hakim F, Kaitsuka T, Raeed J, Wei F, Shiraki N, Akagi T, Yokota T, Kume S, Tomizawa K
    J Biol Chem, 2014;289(14):9623-38.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  40. Sox17-mediated maintenance of fetal intra-aortic hematopoietic cell clusters.
    Authors: Nobuhisa I, Osawa M, Uemura M, Kishikawa Y, Anani M, Harada K, Takagi H, Saito K, Kanai-Azuma M, Kanai Y, Iwama A, Taga T
    Mol Cell Biol, 2014;34(11):1976-90.
    Species: Mouse
    Sample Type: Embryo
    Application: IHC OCT-embedded
  41. A modified EpiSC culture condition containing a GSK3 inhibitor can support germline-competent pluripotency in mice.
    Authors: Tsukiyama, Tomoyuki, Ohinata, Yasuhide
    PLoS ONE, 2014;9(4):e95329.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  42. Unfolded protein response is required for the definitive endodermal specification of mouse embryonic stem cells via Smad2 and beta-catenin signaling.
    Authors: Xu H, Tsang K, Wang Y, Chan J, Xu G, Gao W
    J Biol Chem, 2014;289(38):26290-301.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Not Specified
  43. Generation and characterization of induced pluripotent stem cells from Aid-deficient mice.
    Authors: Shimamoto R, Amano N, Ichisaka T, Watanabe A, Yamanaka S, Okita K
    PLoS ONE, 2014;9(4):e94735.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  44. Identification of transcription factors for lineage-specific ESC differentiation.
    Authors: Yamamizu K, Piao Y, Sharov A, Zsiros V, Yu H, Nakazawa K, Schlessinger D, Ko M
    Stem Cell Reports, 2013;1(6):545-59.
  45. Cell-cycle control of developmentally regulated transcription factors accounts for heterogeneity in human pluripotent cells.
    Authors: Singh A, Chappell J, Trost R, Lin L, Wang T, Tang J, Matlock B, Weller K, Wu H, Zhao S, Jin P, Dalton S
    Stem Cell Reports, 2013;1(6):532-44.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  46. Defined extracellular matrix components are necessary for definitive endoderm induction.
    Authors: Taylor-Weiner H, Schwarzbauer J, Engler A
    Stem Cells, 2013;31(10):2084-94.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  47. Production of hepatocyte-like cells from human pluripotent stem cells.
    Authors: Hannan, Nicholas, Segeritz, Charis-P, Touboul, Thomas, Vallier, Ludovic
    Nat Protoc, 2013;8(2):430-7.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  48. CD166(pos) subpopulation from differentiated human ES and iPS cells support repair of acute lung injury.
    Authors: Soh, Boon Sen, Zheng, Dahai, Li Yeo, Julie Su, Yang, Henry He, Ng, Shi Yan, Wong, Lan Hion, Zhang, Wencai, Li, Pin, Nichane, Massimo, Asmat, Atasha, Wong, Poo Sing, Wong, Peng Che, Su, Lin Lin, Mantalaris, Sakis A, Lu, Jia, Xian, Wa, McKeon, Frank, Chen, Jianzhu, Lim, Elaine H, Lim, Bing
    Mol Ther, 2012;20(12):2335-46.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  49. Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme.
    Authors: Sneddon, Julie B, Borowiak, Malgorza, Melton, Douglas
    Nature, 2012;491(7426):765-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  50. A molecular signature for purified definitive endoderm guides differentiation and isolation of endoderm from mouse and human embryonic stem cells.
    Authors: Wang P, McKnight K, Wong D, Rodriguez R, Sugiyama T, Gu X, Ghodasara A, Qu K, Chang H, Kim S
    Stem Cells Dev, 2012;21(12):2273-87.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  51. The endothelial cell line bEnd.3 maintains human pluripotent stem cells.
    Authors: Joubin, Katherin, Richardson, Amelia, Novoa, Natalia, Tu, Edmund, Tomishima, Mark J
    Stem Cells Dev, 2012;21(12):2312-21.
    Species: Human
    Sample Type: Whole Cells
  52. CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived from human embryonic stem cells.
    Authors: Jiang W, Sui X, Zhang D, Liu M, Ding M, Shi Y, Deng H
    Stem Cells, 2011;29(4):609-17.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  53. A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3.
    Authors: Bone HK, Nelson AS, Goldring CE, Tosh D, Welham MJ
    J. Cell. Sci., 2011;124(0):1992-2000.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  54. Early in vitro differentiation of mouse definitive endoderm is not correlated with progressive maturation of nuclear DNA methylation patterns.
    Authors: Tajbakhsh J, Gertych A, Fagg WS
    PLoS ONE, 2011;6(7):e21861.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  55. The ROCK inhibitor Y-27632 improves recovery of human embryonic stem cells after fluorescence-activated cell sorting with multiple cell surface markers.
    Authors: Emre N, Vidal JG, Elia J
    PLoS ONE, 2010;5(8):e12148.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  56. SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cells.
    Authors: Chng Z, Teo A, Pedersen RA, Vallier L
    Cell Stem Cell, 2010;6(1):59-70.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  57. A role for PDGF signaling in expansion of the extra-embryonic endoderm lineage of the mouse blastocyst.
    Authors: Artus J, Panthier JJ, Hadjantonakis AK
    Development, 2010;137(20):3361-72.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  58. Origin and formation of the first two distinct cell types of the inner cell mass in the mouse embryo.
    Authors: Morris SA, Teo RT, Li H, Robson P, Glover DM, Zernicka-Goetz M
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(14):6364-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  59. Alginate microcapsule for propagation and directed differentiation of hESCs to definitive endoderm.
    Authors: Chayosumrit M, Tuch B, Sidhu K
    Biomaterials, 2010;31(3):505-14.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  60. Induction of pluripotent stem cells from human third molar mesenchymal stromal cells.
    Authors: Oda Y, Yoshimura Y, Ohnishi H
    J. Biol. Chem., 2010;285(38):29270-8.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  61. A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells.
    Authors: Hansson M, Olesen DR, Peterslund JM, Engberg N, Kahn M, Winzi M, Klein T, Maddox-Hyttel P, Serup P
    Dev. Biol., 2009;330(2):286-304.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  62. Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells.
    Authors: Borowiak M, Maehr R, Chen S, Chen AE, Tang W, Fox JL, Schreiber SL, Melton DA
    Cell Stem Cell, 2009;4(4):348-58.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  63. Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
    Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH
    FEBS Lett., 2009;583(3):561-7.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  64. Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells.
    Authors: Sun N, Panetta NJ, Gupta DM, Wilson KD, Lee A, Jia F, Hu S, Cherry AM, Robbins RC, Longaker MT, Wu JC
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(37):15720-5.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  65. Biphasic induction of Pdx1 in mouse and human embryonic stem cells can mimic development of pancreatic beta-cells.
    Authors: Bernardo AS, Cho CH, Mason S, Docherty HM, Pedersen RA, Vallier L, Docherty K
    Stem Cells, 2009;27(2):341-51.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  66. Highly efficient differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells.
    Authors: Zhang D, Jiang W, Liu M, Sui X, Yin X, Chen S, Shi Y, Deng H
    Cell Res., 2009;19(4):429-38.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  67. High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells.
    Authors: Desbordes SC, Placantonakis DG, Ciro A, Socci ND, Lee G, Djaballah H, Studer L
    Cell Stem Cell, 2008;2(6):602-12.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  68. Prospective isolation and global gene expression analysis of definitive and visceral endoderm.
    Authors: Sherwood RI, Jitianu C, Cleaver O, Shaywitz DA, Lamenzo JO, Chen AE, Golub TR, Melton DA
    Dev. Biol., 2007;304(2):541-55.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  69. Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions.
    Authors: Yao S, Chen S, Clark J, Hao E, Beattie GM, Hayek A, Ding S
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(18):6907-12.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 69 Citations
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedInduced Pluripotent Stem Cells,Endoderm
SpeciesHuman

Other Experimental Details

Other Experimental DetailsUsed this antibody to verify the endodermal germ layer derived from iPSC.

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