Human SRPK3 Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human SRPK3 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 722516
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human SRPK3
    Arg247-Ser316
    Accession # Q9UPE1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human SRPK3 by Western Blot. Western blot shows lysates of human skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SRPK3 Monoclonal Antibody (Catalog # MAB72301) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SRPK3 at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
SRPK3 in Human Skeletal Muscle. SRPK3 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Human SRPK3 Monoclonal Antibody (Catalog # MAB72301) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SRPK3
SRPK3 (Ser/Arg-rich protein specific kinase 3), also called MSSK-1 (muscle-specific serine kinase 1) or STK23 (Ser/Thr kinase 23) is a 59 kDa (predicted) cytoplasmic member of the CMGC Ser/Thr protein kinase family. SRPK3 is principally expressed in heart and skeletal muscle. SRPKs selectively phosphorylate Ser on RS domain-containing proteins, initiating mRNA splicing and maturation.  The 533 amino acid (aa) human SRPK3 contains a split kinase domain (aa 79‑292 and 347‑531) separated by a hinge region. A 491 aa isoform shows an alternate translation start site at aa 43. Within the region used as an immunogen, human SRPK3 shares 87% and 86% aa identity with mouse and rat SRPK3, respectively.
  • Long Name:
    Ser/Arg-rich Protein-specific Kinase 3
  • Entrez Gene IDs:
    26576 (Human); 56504 (Mouse); 293854 (Rat)
  • Alternate Names:
    MGC102944; MSSK1; MSSK-1; MSSK1EC 2.7.11.1; Muscle-specific serine kinase 1; serine arginine rich protein-specific kinase 3; Serine/arginine-rich protein-specific kinase 3; serine/threonine kinase 23; Serine/threonine-protein kinase 23; serine/threonine-protein kinase SRPK3; SFRS protein kinase 3; SRPK3; SR-protein-specific kinase 3; SRSF protein kinase 3; STK23

FAQs

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NorthernLights Guard Mounting Media

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Isotype Controls
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Mouse IgG1 Isotype Control

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Mouse IgG1 Isotype Control

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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Mouse IgG Biotinylated Antibody

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Mouse IgG Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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