Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 722516
View all SRPK3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SRPK3
Arg247-Ser316
Accession # Q9UPE1

Specificity

Detects human SRPK3 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human SRPK3 Antibody

Detection of Human SRPK3 antibody by Western Blot.

Detection of Human SRPK3 by Western Blot.

Western blot shows lysates of human skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SRPK3 Monoclonal Antibody (Catalog # MAB72301) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SRPK3 at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
SRPK3 antibody in Human Skeletal Muscle by Immunohistochemistry (IHC-P).

SRPK3 in Human Skeletal Muscle.

SRPK3 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Human SRPK3 Monoclonal Antibody (Catalog # MAB72301) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human SRPK3 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skeletal muscle

Western Blot

1 µg/mL
Sample: Human skeletal muscle tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SRPK3

SRPK3 (Ser/Arg-rich protein specific kinase 3), also called MSSK-1 (muscle-specific serine kinase 1) or STK23 (Ser/Thr kinase 23) is a 59 kDa (predicted) cytoplasmic member of the CMGC Ser/Thr protein kinase family. SRPK3 is principally expressed in heart and skeletal muscle. SRPKs selectively phosphorylate Ser on RS domain-containing proteins, initiating mRNA splicing and maturation.  The 533 amino acid (aa) human SRPK3 contains a split kinase domain (aa 79‑292 and 347‑531) separated by a hinge region. A 491 aa isoform shows an alternate translation start site at aa 43. Within the region used as an immunogen, human SRPK3 shares 87% and 86% aa identity with mouse and rat SRPK3, respectively.

Long Name

Ser/Arg-rich Protein-specific Kinase 3

Alternate Names

MSSK1, STK23

Entrez Gene IDs

26576 (Human); 56504 (Mouse); 293854 (Rat)

Gene Symbol

SRPK3

UniProt

Q9UPE1 (Human)

Additional SRPK3 Products

Product Documents for Human SRPK3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SRPK3 Antibody

For research use only

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Protocols

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