Human ST8 alpha -2,8‑Sialyltransferase 8A/ST8SIA1 Antibody AF6716: R&D Systems

Human ST8 alpha -2,8‑Sialyltransferase 8A/
ST8SIA1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human ST8 alpha -2,8‑Sialyltransferase 8A/ST8SIA1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human ST8 alpha -2,8‑Sialyltransferase 8A/ST8SIA1
    Tyr49-Ser356
    Accession # Q92185
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human ST8  alpha ‑2,8‑Sialyltransferase 8A/
ST8SIA1 by Western Blot.

Western blot shows lysates of Hs 294T human melanoma cell line, SK‑Mel‑28 human malignant melanoma cell line, A375 human melanoma cell line, and Bowes human melanoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human ST8 alpha ‑2,8‑Sialyltransferase 8A/ST8SIA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6716) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST8 alpha ‑2,8‑Sialyltransferase 8A/ST8SIA1 at approximately 48‑50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry
ST8  alpha ‑2,8‑Sialyltransferase 8A/ST8SIA1 in Human Breast Cancer Tissue.

ST8 alpha ‑2,8‑Sialyltransferase 8A/ST8SIA1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human ST8 alpha ‑2,8‑Sialyltransferase 8A/ST8SIA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6716) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ST8 alpha-2,8-Sialyltransferase 8A/ST8SIA1
Gangliosides are acidic glycosphingolipids that contain one or more sialic acid residues and are particularly prevalent on neuronal cells (1). Ganglioside GD3 is involved in cell adhesion and the growth of cultured malignant cells (2). ST8SIA1 is a sialyltransferase that catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 (NeuNAc alpha 2‑3Gal beta 1‑4Glc‑Cer) to produce GD3 (NeuNAc alpha 2‑8NeuNAc alpha 2‑3Gal beta 1‑4Glc‑Cer) and GT3 (NeuNAc alpha 2‑8NeuNAc alpha 2‑8NeuNAc alpha 2‑3Gal beta 1‑4Glc‑Cer) in a successive manner (3); therefore the enzyme has both GD3 and GT3 synthase activity (4). ST8SIA1 is mainly expressed in adult and fetal brain, and its expression is enhanced in melanoma cell lines (3, 4, 5). Like most known glycosyltransferases, ST8SIA1 is predicted as a type II transmembrane protein with a short N‑terminal cytoplasmic domain and a single-pass transmembrane domain followed by an enzymatic domain in the lumen of the Golgi apparatus. However, recently GD3 synthase activity was demonstrated at the surface of epithelial and melanoma cells, suggesting glycosphingolipid synthesis may occur at the cell membrane (6). Recombinant ST8SIA1 also showed activity on fetuin from fetal calf serum, when measured using a phosphatase-coupled method (7).
  • References:
    1. Kolter, T. et al. (2002) J. Biol. Chem. 277:25859.
    2. Cheresh, D.A. et al. (1986) J. Cell. Biol. 102:688.
    3. Nakayama, J. et al. (1986) J. Biol. Chem. 271:3684.
    4. Nara, K. et al. (1994) Proc. Natl. Acad. Sci. USA. 91:7952.
    5. Haraguchi, M. et al. (1994) Proc. Natl. Acad. Sci. USA. 91:10455.
    6. Crespo, P.M. et al. (2010) J. Biol. Chem. 285:29179.
    7. Wu, Z.L. et al. (2010) Glycobiology doi: 10.1093/glycob/cwq187.
  • Long Name:
    ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1
  • Entrez Gene IDs:
    6489 (Human); 20449 (Mouse); 25280 (Rat)
  • Alternate Names:
    Alpha-2,8-sialyltransferase 8A; alpha-N-acetylneuraminide alpha-2,8-sialyltransferase; disialoganglioside (GD3) synthase; Ganglioside GD3 synthase; Ganglioside GT3 synthase; ganglioside-specific alpha-2,8-polysialyltransferase; GD3 synthase); GD3S; sialyltransferase 8 (alpha-N-acetylneuraminate: alpha-2,8-sialytransferase, GD3synthase) A; sialyltransferase 8A (alpha-N-acetylneuraminate: alpha-2,8-sialyltransferase; Sialyltransferase 8A; Sialytransferase St8Sia I; SIAT8A; SIAT8-A; SIAT8EC 2.4.99.8; ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1; ST8SIA1; ST8SiaI
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ST8SIA1 Antibody?

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