Human T-bet/TBX21 Antibody

Catalog # Availability Size / Price Qty
Detection of T‑bet/TBX21 in Jurkat Human Cell Line by Flow Cytometry.
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Product Details
Citations (3)
Supplemental Products
Reviews (1)

Human T-bet/TBX21 Antibody Summary

Species Reactivity
Detects human T-bet.
Monoclonal Mouse IgG1 Clone # 525803
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human T-bet
Accession # Q9UL17
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
8-25 µg/mL
Immersion fixed Jurkat human acute T cell leukemia cell line
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of T‑bet/TBX21 antibody in Jurkat Human Cell Line antibody by Flow Cytometry. View Larger

Detection of T‑bet/TBX21 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Human T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB5385, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with ice-cold methanol.

Western Blot Detection of Human T-bet/TBX21 antibody by Western Blot. View Larger

Detection of Human T-bet/TBX21 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Human T-bet/TBX21 Monoclonal Antibody (Catalog # MAB5385) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for T-bet/TBX21 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: T-bet/TBX21

T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62 kDa member of the T-box family of transcription factors and the Tbr1 subfamily. Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1-cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN gamma, and initiates Th1 lineage development from naïve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.

Long Name
T-box transcription factor TBX21
Entrez Gene IDs
30009 (Human); 57765 (Mouse); 303496 (Rat)
Alternate Names
Tbet; T-bet; TBETT-PET; TBLYM; TBLYMT-cell-specific T-box transcription factor T-bet; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor TBX21; TBX21; T-PET; Transcription factor TBLYM

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Citations for Human T-bet/TBX21 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The effect of adalimumab on key drivers in the pathogenesis of psoriasis.
    Authors: Hendriks A, van der Velden H, Wolberink E, Seyger M, Schalkwijk J, Zeeuwen P, de Jong E, Pasch M, van Erp P, van de Kerkhof P
    Br J Dermatol, 2014;170(3):571-80.
  2. Regional immunity in melanoma: immunosuppressive changes precede nodal metastasis.
    Authors: Mansfield AS, Holtan SG, Grotz TE
    Mod. Pathol., 2011;24(4):487-94.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. TLR2 Stimulation Drives Human Naive and Effector Regulatory T Cells into a Th17-Like Phenotype with Reduced Suppressive Function.
    Authors: Nyirenda MH, Sanvito L, Darlington PJ
    J. Immunol., 2011;187(5):2278-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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Human T-bet/TBX21 Antibody
By Anonymous on 06/11/2022
Application: WB Sample Tested: Daudi human Burkitt's lymphoma cell line Species: Human