Human T-bet/TBX21 Antibody Summary
Accession # Q9UL17
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of T‑bet/TBX21 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Human T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB5385, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with ice-cold methanol.
Detection of Human T-bet/TBX21 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Human T-bet/TBX21 Monoclonal Antibody (Catalog # MAB5385) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for T-bet/TBX21 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62 kDa member of the T-box family of transcription factors and the Tbr1 subfamily. Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1-cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN gamma, and initiates Th1 lineage development from naïve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.
Citations for Human T-bet/TBX21 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
Filter your results:
The effect of adalimumab on key drivers in the pathogenesis of psoriasis.
Authors: Hendriks A, van der Velden H, Wolberink E, Seyger M, Schalkwijk J, Zeeuwen P, de Jong E, Pasch M, van Erp P, van de Kerkhof P
Br J Dermatol, 2014;170(3):571-80.
Regional immunity in melanoma: immunosuppressive changes precede nodal metastasis.
Authors: Mansfield AS, Holtan SG, Grotz TE
Mod. Pathol., 2011;24(4):487-94.
Sample Types: Whole Tissue
TLR2 Stimulation Drives Human Naive and Effector Regulatory T Cells into a Th17-Like Phenotype with Reduced Suppressive Function.
Authors: Nyirenda MH, Sanvito L, Darlington PJ
J. Immunol., 2011;187(5):2278-90.
Sample Types: Whole Cells
Applications: Flow Cytometry
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Cell and Tissue Staining Kits
Reviews for Human T-bet/TBX21 Antibody
There are currently no reviews for this product. Be the first to review Human T-bet/TBX21 Antibody and earn rewards!
Have you used Human T-bet/TBX21 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image