Human TACI/TNFRSF13B Biotinylated Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human TACI/TNFRSF13B in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant mouse TACI is observed and less than 0.2% cross-reactivity with recombinant human BCMA is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TACI/TNFRSF13B
    Ser2-Thr166
    Accession # Q53F36
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC)
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed paraffin-embedded sections of human spleen subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
    • Human TACI/TNFRSF13B Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human TACI/TNFRSF13B Antibody (Catalog # MAB174)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human TACI/TNFRSF13B Biotinylated Antibody (Catalog # BAF174)
  • ELISA Standard
     
    Recombinant Human TACI/TNFRSF13B Fc Chimera Protein, CF (Catalog # 174-TC)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TACI/TNFRSF13B

TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily. TACI is a type III membrane protein with an extracellular N‑terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine rich domains. Within the TNFRSF, it shares the highest homology with BCMA. TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Human TACI is a 293 amino acid (aa) protein consisting of a 166 aa extracellular domain, a 20 aa transmembrane domain, and a 107 aa intracellular domain. Human and mouse TACI share 54% amino acid identity.

  • References:
    1. Xia, X.-Z. et al. (2000) J. Exp. Med. 192:137.
    2. von Bulow, G.U. et al. (1997) Science, 278:138.
    3. Gross, J.A. et al. (2000) Nature 404:995.
    4. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
    5. Yan, M. et al. (2000) Nature Immunol. 1:37.
    6. Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.
  • Long Name:
    Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B
  • Entrez Gene IDs:
    23495 (Human); 57916 (Mouse)
  • Alternate Names:
    CD267 antigen; CD267; CVID; CVID2; FLJ39942; MGC133214; TACI; TACIMGC39952; TNFRSF13B; TNFRSF14B; Transmembrane activator and CAML interactor; tumor necrosis factor receptor 13B; tumor necrosis factor receptor superfamily member 13B; tumor necrosis factor receptor superfamily, member 13B
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Fusion Toxin BLyS-Gelonin Inhibits Growth of Malignant Human B Cell Lines In Vitro and In Vivo.
    Authors: Luster, Troy A, Mukherjee, Ipsita, Carrell, Jeffrey, Cho, Yun Hee, Gill, Jeffrey, Kelly, Lizbeth, Garcia, Andy, Ward, Christop, Oh, Luke, Ullrich, Stephen, Migone, Thi-Sau, Humphreys, Robin
    PLoS ONE, 2012;7(10):e47361.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Atacicept in patients with rheumatoid arthritis: results of a multicenter, phase Ib, double-blind, placebo-controlled, dose-escalating, single- and repeated-dose study.
    Authors: Tak PP, Thurlings RM, Rossier C, Nestorov I, Dimic A, Mircetic V, Rischmueller M, Nasonov E, Shmidt E, Emery P, Munafo A
    Arthritis Rheum., 2008;58(1):61-72.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
  3. IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells.
    Authors: Ettinger R, Sims GP, Robbins R, Withers D, Fischer RT, Grammer AC, Kuchen S, Lipsky PE
    J. Immunol., 2007;178(5):2872-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. B-lymphocyte stimulator (BLyS) stimulates immunoglobulin production and malignant B-cell growth in Waldenstrom macroglobulinemia.
    Authors: Elsawa SF, Novak AJ, Grote DM, Ziesmer SC, Witzig TE, Kyle RA, Dillon SR, Harder B, Gross JA, Ansell SM
    Blood, 2006;107(7):2882-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Decreased survival of B cells of HIV-viremic patients mediated by altered expression of receptors of the TNF superfamily.
    Authors: Moir S, Malaspina A, Pickeral OK, Donoghue ET, Vasquez J, Miller NJ, Krishnan SR, Planta MA, Turney JF, Justement JS, Kottilil S, Dybul M, Mican JM, Kovacs C, Chun TW, Birse CE, Fauci AS
    J. Exp. Med., 2004;200(7):587-99.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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