Human TACI/TNFRSF13B Antibody

  (2 citations)     
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Citations (2)
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  • Species Reactivity
    Human
  • Specificity
    Detects human TACI/TNFRSF13B in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse TACI or recombinant human BCMA is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 165609
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TACI/TNFRSF13B
    Ser2-Thr166
    Accession # O14836
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC)
  • Immunohistochemistry
    8-25 µg/mL
    Immersion fixed paraffin-embedded sections of human spleen
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 0.03-0.12 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) to immobilized Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
    • Human TACI/TNFRSF13B Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human TACI/TNFRSF13B Antibody (Catalog # MAB174 )
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human TACI/TNFRSF13B Biotinylated Antibody (Catalog # BAF174 )
  • ELISA Standard
     
    Recombinant Human TACI/TNFRSF13B Fc Chimera Protein, CF (Catalog # 174-TC )
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TACI/TNFRSF13B

TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily. TACI is a type III membrane protein with an extracellular N‑terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine rich domains. Within the TNFRSF, it shares the highest homology with BCMA. TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Human TACI is a 293 amino acid (aa) protein consisting of a 166 aa extracellular domain, a 20 aa transmembrane domain, and a 107 aa intracellular domain. Human and mouse TACI share 54% amino acid identity.

  • References:
    1. Xia, X.-Z. et al. (2000) J. Exp. Med. 192:137.
    2. von Bulow, G.U. et al. (1997) Science, 278:138.
    3. Gross, J.A. et al. (2000) Nature 404:995.
    4. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
    5. Yan, M. et al. (2000) Nature Immunol. 1:37.
    6. Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.
  • Long Name:
    Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B
  • Entrez Gene IDs:
    23495 (Human); 57916 (Mouse)
  • Alternate Names:
    CD267 antigen; CD267; CVID; CVID2; FLJ39942; MGC133214; TACI; TACIMGC39952; TNFRSF13B; TNFRSF14B; Transmembrane activator and CAML interactor; tumor necrosis factor receptor 13B; tumor necrosis factor receptor superfamily member 13B; tumor necrosis factor receptor superfamily, member 13B
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. The immunoregulator soluble TACI is released by ADAM10 and reflects B cell activation in autoimmunity.
    Authors: Hoffmann F, Kuhn P, Laurent S, Hauck S, Berer K, Wendlinger S, Krumbholz M, Khademi M, Olsson T, Dreyling M, Pfister H, Alexander T, Hiepe F, Kumpfel T, Crawford H, Wekerle H, Hohlfeld R, Lichtenthaler S, Meinl E
    J Immunol, 2015;194(2):542-52.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  2. BlyS is up-regulated by hypoxia and promotes migration of human breast cancer cells.
    Authors: Zhu J, Sun L, Lin S
    J. Exp. Clin. Cancer Res., 2012;31(0):31.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

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