Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Xenograft

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Tankyrase 1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Tankyrase 1
Ala943-Gly1039
Accession # O95271

Specificity

Detects human Tankyrase 1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Tankyrase 1 Antibody

Detection of Human Tankyrase 1 antibody by Western Blot.

Detection of Human Tankyrase 1 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Tankyrase 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7116) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tankyrase 1 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Tankyrase 1 by Western Blot

Detection of Tankyrase 1 by Western Blot

Tankyrase inhibition stabilizes AXIN levels and decreases beta -catenin in human HCC cell linesA. HepG2, B. Huh7, and C. Hep40 cells were treated for 24 hours with 10 μM XAV939, 10 μM WXL-8, or DMSO as control, and whole cell extracts isolated for Western blotting using anti-TNKS1, anti-AXIN1, anti-AXIN2, anti-beta -catenin, and anti-survivin. GAPDH was used as the loading control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26246473), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Tankyrase 1 by Western Blot

Detection of Tankyrase 1 by Western Blot

Tankyrase inhibition stabilizes AXIN levels and decreases beta -catenin in human HCC cell linesA. HepG2, B. Huh7, and C. Hep40 cells were treated for 24 hours with 10 μM XAV939, 10 μM WXL-8, or DMSO as control, and whole cell extracts isolated for Western blotting using anti-TNKS1, anti-AXIN1, anti-AXIN2, anti-beta -catenin, and anti-survivin. GAPDH was used as the loading control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26246473), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Tankyrase 1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: MCF‑7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tankyrase 1

TNKS (Tankyrase; also TRF1-interacting, ANKYrin-Related ADP-ribose polymerase and TNKS1, TANK1 and PARP5A) is a 165-170 kDa member of the PARP family of proteins. It is ubiquitously expressed and participates in at least two critical cellular functions. First, via ribosylation activity, it inhibits TRF1, a regulator of telomere length. This promotes chromosomal lengthening. Second, it activates the Wnt signaling pathway by promoting Axin-1 and -2 degradation, resulting in beta -catenin activation. Human TNKS is 1327 amino acids (aa) in length. It contains polyHis, Pro and Ser regions (aa 9-134), 15 ankyrin repeats (aa 215-934), one SAM motif (aa 1030-1089) and a PARP catalytic domain (aa 1112-1317). There are three potential splice variants. One shows a three aa substitution for aa 640-1327, another contains a 43 aa substitution for aa 1-327, while a third possesses a 20 aa substitution for aa 300-327. Over aa 943-1039, human TNKS shares 97% aa identity with mouse TNKS.

Long Name

TRF1-interacting Ankyrin-related ADP-ribose Polymerase

Alternate Names

PARP5A, PARPL, pART5, TANK1, TINF1, TNKS

Entrez Gene IDs

8658 (Human); 21951 (Mouse); 290794 (Rat)

Gene Symbol

TNKS

UniProt

O95271 (Human)

Additional Tankyrase 1 Products

Product Documents for Human Tankyrase 1 Antibody

Certificate of Analysis

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Product Specific Notices for Human Tankyrase 1 Antibody

For research use only

Citations for Human Tankyrase 1 Antibody

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