Home / Tankyrase 1 / Human Tankyrase 1 Antibody

Human Tankyrase 1 Antibody

Catalog #: AF7116
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF7116
AF7116-SP

Save 15% on Select RUO Reagents. See Details. 

Detection of Human Tankyrase 1 by Western Blot.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Tankyrase 1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Tankyrase 1 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Tankyrase 1
Ala943-Gly1039
Accession # O95271
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Tankyrase 1 antibody by Western Blot. View Larger

Detection of Human Tankyrase 1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Tankyrase 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7116) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tankyrase 1 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tankyrase 1

TNKS (Tankyrase; also TRF1-interacting, ANKYrin-Related ADP-ribose polymerase and TNKS1, TANK1 and PARP5A) is a 165-170 kDa member of the PARP family of proteins. It is ubiquitously expressed and participates in at least two critical cellular functions. First, via ribosylation activity, it inhibits TRF1, a regulator of telomere length. This promotes chromosomal lengthening. Second, it activates the Wnt signaling pathway by promoting Axin-1 and -2 degradation, resulting in beta -catenin activation. Human TNKS is 1327 amino acids (aa) in length. It contains polyHis, Pro and Ser regions (aa 9-134), 15 ankyrin repeats (aa 215-934), one SAM motif (aa 1030-1089) and a PARP catalytic domain (aa 1112-1317). There are three potential splice variants. One shows a three aa substitution for aa 640-1327, another contains a 43 aa substitution for aa 1-327, while a third possesses a 20 aa substitution for aa 300-327. Over aa 943-1039, human TNKS shares 97% aa identity with mouse TNKS.

Long Name
TRF1-interacting Ankyrin-related ADP-ribose Polymerase
Entrez Gene IDs
8658 (Human); 21951 (Mouse); 290794 (Rat)
Alternate Names
EC 2.4.2.30; PARP5A; PARP-5a; PARP5APARPL; PARPL; pART5; Poly [ADP-ribose] polymerase 5A; TANK1; Tankyrase 1; Tankyrase I; tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase; tankyrase-1; TIN1TANK1; TINF1; TINF1TNKS-1; TNKS; TNKS1TRF1-interacting ankyrin-related ADP-ribose polymerase

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Human Tankyrase 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Tankyrase inhibitors attenuate WNT/ beta -catenin signaling and inhibit growth of hepatocellular carcinoma cells
    Authors: Li Ma, Xiaolin Wang, Tao Jia, Wei Wei, Mei-Sze Chua, Samuel So
    Oncotarget

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Tankyrase 1 Antibody

There are currently no reviews for this product. Be the first to review Human Tankyrase 1 Antibody and earn rewards!

Have you used Human Tankyrase 1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review