hh Human TGF-beta  RIII Antibody AF-242-PB: R&D Systems

Human TGF-beta  RIII Antibody

(7 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human TGF-beta  RIII in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) TGF-beta  RIII is observed, and less than 1% cross-reactivity with recombinant human (rh) TGF-beta  RI, rhTGF-beta  RII, rhTGF-beta  RIIB, and rmTGF-beta  RI  is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TGF‑ beta  RIII
    Gly21-Asp781
    Accession # AAA67061
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human TGF-beta  RIII (Catalog # 242-R3)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TGF‑ beta  RIII in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Goat Anti-Human TGF‑ beta  RIII Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF‑242‑PB, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Fluorescein-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0109).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta RIII

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type I receptor is a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. The Type II receptor is also a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I and Type II receptors together form a heterodimeric signaling complex that is essential for the transduction of the anti-proliferative signals of TGF-beta.

The Type III receptor is a transmembrane proteoglycan with a large extracellular domain and a 43 amino acid residue cytoplasmic domain. The cytoplasmic domain of the Type III receptor lacks an obvious signaling motif and the receptor may not be involved directly in signal transduction. The Type III receptor binds TGF-beta 2 with the highest affinity. Other TGF-beta isoforms also bind the Type III receptor, but with lower affinities. Cellular responsiveness to TGF-beta 2 appears to be dependent on the presence of the Type III receptor which can interact with the signaling receptor complex. In addition to the transmembrane Type III receptor, a soluble form of the receptor is secreted by some cell types. The physiological role of this soluble receptor remains to be determined. The recombinant TGF-beta soluble receptor Type III binds the TGF-beta isoforms differentially in solution and exhibits TGF-beta antagonistic activities in vitro.

  • Long Name:
    Transforming Growth Factor beta Receptor III
  • Entrez Gene IDs:
    7049 (Human); 21814 (Mouse); 29610 (Rat)
  • Alternate Names:
    betaglycan proteoglycan; Betaglycan; BGCAN; TBRIII; TGF-beta receptor type 3; TGF-beta receptor type III; TGF-beta RIII; TGFbetaRIII; TGFBR3; TGF-bRIII; TGFR-3; Transforming growth factor beta receptor III; transforming growth factor beta receptor type 3; transforming growth factor, beta receptor III (betaglycan, 300kDa); transforming growth factor, beta receptor III
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. TWIST1-Induced miR-424 Reversibly Drives Mesenchymal Programming while Inhibiting Tumor Initiation.
    Authors: Drasin D, Guarnieri A, Neelakantan D, Kim J, Cabrera J, Wang C, Zaberezhnyy V, Gasparini P, Cascione L, Huebner K, Tan A, Ford H
    Cancer Res, 2015;75(9):1908-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Molecular signatures and transcriptional regulatory networks of human immature decidual NK and mature peripheral NK cells.
    Authors: Wang F, Zhou Y, Fu B, Wu Y, Zhang R, Sun R, Tian Z, Wei H
    Eur J Immunol, 2014;44(9):2771-84.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration.
    Authors: Murphy MO, Ghosh J, Fulford P, Khwaja N, Halka AT, Carter A, Turner NJ, Walker MG
    Eur J Vasc Endovasc Surg, 2006;31(5):516-22.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  4. Inhibin alpha-subunit and the inhibin coreceptor betaglycan are downregulated in endometrial carcinoma.
    Authors: Florio P, Ciarmela P, Reis FM, Toti P, Galleri L, Santopietro R, Tiso E, Tosi P, Petraglia F
    Eur. J. Endocrinol., 2005;152(2):277-84.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. Extracellular domain of TGFbeta type III receptor inhibits angiogenesis and tumor growth in human cancer cells.
    Authors: Bandyopadhyay A, Zhu Y, Malik SN, Kreisberg J, Brattain MG, Sprague EA, Luo J, Lopez-Casillas F, Sun LZ
    Oncogene, 2002;21(22):3541-51.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  6. TGF-beta1 regulates TGF-beta1 and FGF-2 mRNA expression during fibroblast wound healing.
    Authors: Song QH, Klepeis VE, Trinkaus-Randall V
    MP, Mol. Pathol., 2002;55(3):164-76.
    Species: Rabbit
    Sample Type: Whole Tissue
    Application: IHC
  7. Transforming growth factor-beta1 regulation of growth zone chondrocytes is mediated by multiple interacting pathways.
    Authors: Rosado E, Schwartz Z, Sylvia VL, Dean DD, Boyan BD
    Biochim. Biophys. Acta, 2002;1590(1):1-15.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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