Human TGF-beta RIII Antibody
Human TGF-beta RIII Antibody Summary
Gly21-Asp781
Accession # AAA67061
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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TGF-beta RIII in Human Placenta Tissue. TGF-beta RIII was detected in immersion fixed paraffin-embedded sections of human placenta tissue using Goat Anti-Human TGF-beta RIII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-242-PB) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface on syncytiotrophoblast cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of TGF‑ beta RIII in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Goat Anti-Human TGF-beta RIII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-242-PB, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Fluorescein-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0109).
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Detection of TGF-beta RIII in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast adenocarcinoma cell line was stained with Goat Anti-Human TGF-beta RIII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-242-PB, filled histogram) or control antibody (AB-108-C, open histogram), followed by PE-conjugated Anti-Goat IgG Secondary Antibody (F0107).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta RIII
Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type I receptor is a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. The Type II receptor is also a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I and Type II receptors together form a heterodimeric signaling complex that is essential for the transduction of the anti-proliferative signals of TGF-beta.
The Type III receptor is a transmembrane proteoglycan with a large extracellular domain and a 43 amino acid residue cytoplasmic domain. The cytoplasmic domain of the Type III receptor lacks an obvious signaling motif and the receptor may not be involved directly in signal transduction. The Type III receptor binds TGF-beta 2 with the highest affinity. Other TGF-beta isoforms also bind the Type III receptor, but with lower affinities. Cellular responsiveness to TGF-beta 2 appears to be dependent on the presence of the Type III receptor which can interact with the signaling receptor complex. In addition to the transmembrane Type III receptor, a soluble form of the receptor is secreted by some cell types. The physiological role of this soluble receptor remains to be determined. The recombinant TGF-beta soluble receptor Type III binds the TGF-beta isoforms differentially in solution and exhibits TGF-beta antagonistic activities in vitro.
Product Datasheets
Citations for Human TGF-beta RIII Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Evidence for a pathogenic role of extrafollicular, IL-10-producing CCR6+B helper T cells in systemic lupus erythematosus
Authors: F Facciotti, P Larghi, R Bosotti, C Vasco, N Gagliani, C Cordiglier, S Mazzara, V Ranzani, E Rottoli, S Curti, A Penatti, B Karnani, Y Kobayashi, M Crosti, M Bombaci, JP van Hambur, G Rossetti, R Gualtierot, M Gerosa, S Gatti, S Torretta, L Pignataro, SW Tas, S Abrignani, M Pagani, F Grassi, PL Meroni, RA Flavell, J Geginat
Proc. Natl. Acad. Sci. U.S.A., 2020;117(13):7305-7316.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Altering the Proteoglycan State of Transforming Growth Factor ? Type III Receptor (T?RIII)/Betaglycan Modulates Canonical Wnt/?-Catenin Signaling
Authors: Karthikeyan Mythreye
J. Biol. Chem., 2016;291(49):25716-25728.
Applications: Immunoprecipitation -
TWIST1-Induced miR-424 Reversibly Drives Mesenchymal Programming while Inhibiting Tumor Initiation.
Authors: Drasin D, Guarnieri A, Neelakantan D, Kim J, Cabrera J, Wang C, Zaberezhnyy V, Gasparini P, Cascione L, Huebner K, Tan A, Ford H
Cancer Res, 2015;75(9):1908-21.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Molecular signatures and transcriptional regulatory networks of human immature decidual NK and mature peripheral NK cells.
Authors: Wang F, Zhou Y, Fu B, Wu Y, Zhang R, Sun R, Tian Z, Wei H
Eur J Immunol, 2014;44(9):2771-84.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration.
Authors: Murphy MO, Ghosh J, Fulford P, Khwaja N, Halka AT, Carter A, Turner NJ, Walker MG
Eur J Vasc Endovasc Surg, 2006;31(5):516-22.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Inhibin alpha-subunit and the inhibin coreceptor betaglycan are downregulated in endometrial carcinoma.
Authors: Florio P, Ciarmela P, Reis FM, Toti P, Galleri L, Santopietro R, Tiso E, Tosi P, Petraglia F
Eur. J. Endocrinol., 2005;152(2):277-84.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
TGF-beta1 regulates TGF-beta1 and FGF-2 mRNA expression during fibroblast wound healing.
Authors: Song QH, Klepeis VE, Trinkaus-Randall V
MP, Mol. Pathol., 2002;55(3):164-76.
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHC -
Transforming growth factor-beta1 regulation of growth zone chondrocytes is mediated by multiple interacting pathways.
Authors: Rosado E, 2019, Schwartz Z, Sylvia VL, Dean DD, Boyan BD
91, 2002;1590(1):1-15.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization -
Extracellular domain of TGFbeta type III receptor inhibits angiogenesis and tumor growth in human cancer cells.
Authors: Bandyopadhyay A, 2019, Zhu Y, Malik SN, Kreisberg J, Brattain MG, Sprague EA, Luo J, Lopez-Casillas F, Sun LZ
101205, 2002;21(22):3541-51.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
FAQs
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What epitope does this antibody detect?
We have not done epitope mapping, but the antibody was raised against the extracellular domain of recombinant human TGF -beta RIII, Gly 21-Asp781, accession # AAA67061. The antibody will react with the antigen in this region.
Isotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for Human TGF-beta RIII Antibody
Average Rating: 3.5 (Based on 2 Reviews)
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Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
