Human TGF-beta  RIII Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF242
Product Details
Citations (1)
FAQs
Supplemental Products
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Human TGF-beta  RIII Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TGF‑ beta  RIII in ELISAs and Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) TGF‑ beta 1, rhTGF‑ beta 2, rhTGF‑ beta 3, recombinant amphibian TGF‑ beta 5, rhLAP (TGF‑ beta 1), rhTGF‑ beta  RII, and rhGDNF is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TGF‑ beta  RIII
Gly21-Asp781
Accession # AAA67061
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TGF-beta  RIII (Catalog # 242-R3)

Human TGF-beta RIII Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-.04 µg/mL 

Use in combination with:

Capture Reagent: Human TGF‑ beta  RIII Antibody (Catalog # MAB2421)

Standard: Recombinant Human TGF-beta RIII Protein (Catalog # 242-R3)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RIII

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type I receptor is a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. The Type II receptor is also a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I and Type II receptors together form a heterodimeric signaling complex that is essential for the transduction of the anti-proliferative signals of TGF-beta.

The Type III receptor is a transmembrane proteoglycan with a large extracellular domain and a 43 amino acid residue cytoplasmic domain. The cytoplasmic domain of the Type III receptor lacks an obvious signaling motif and the receptor may not be involved directly in signal transduction. The Type III receptor binds TGF-beta 2 with the highest affinity. Other TGF-beta isoforms also bind the Type III receptor, but with lower affinities. Cellular responsiveness to TGF-beta 2 appears to be dependent on the presence of the Type III receptor which can interact with the signaling receptor complex. In addition to the transmembrane Type III receptor, a soluble form of the receptor is secreted by some cell types. The physiological role of this soluble receptor remains to be determined. The recombinant TGF-beta soluble receptor Type III binds the TGF-beta isoforms differentially in solution and exhibits TGF-beta antagonistic activities in vitro.

Long Name
Transforming Growth Factor beta Receptor III
Entrez Gene IDs
7049 (Human); 21814 (Mouse); 29610 (Rat)
Alternate Names
betaglycan proteoglycan; Betaglycan; BGCAN; TBRIII; TGF-beta receptor type 3; TGF-beta receptor type III; TGF-beta RIII; TGFbetaRIII; TGFBR3; TGF-bRIII; TGFR-3; Transforming growth factor beta receptor III; transforming growth factor beta receptor type 3; transforming growth factor, beta receptor III (betaglycan, 300kDa); transforming growth factor, beta receptor III

Product Datasheets

Citation for Human TGF-beta  RIII Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Strain-dependent control of transforming growth factor-beta function in osteoblasts in an in vitro model: biochemical events associated with distraction osteogenesis.
    Authors: Knoll BI, McCarthy TL, Centrella M, Shin J
    Plast. Reconstr. Surg., 2005;116(1):224-33.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

  1. What epitope does this antibody detect?

    • We have not done epitope mapping, but the antibody was raised against the extracellular domain of recombinant human TGF -beta RIII, Gly 21-Asp781, accession # AAA67061.  The antibody will react with the antigen in this region.

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