Human TGF-beta  RIII Antibody

Catalog # Availability Size / Price Qty
MAB2421
MAB2421-SP
Product Details
FAQs
Supplemental Products
Reviews (2)

Human TGF-beta  RIII Antibody Summary

Species Reactivity
Human
Specificity
Detects human TGF-beta RIII in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) TGF-beta RI, RII, RIIb, recombinant mouse (rm) TGF-beta  RIII, rhTGF-beta 1, beta 1.2, beta 2, beta 3, rhLatent TGF-beta 1, or rhLAP is observed.
Source
Monoclonal Mouse IgG1 Clone # 31308
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Murine myeloma cell line, NS0-derived recombinant human TGF-beta RIII
Gly21-Asp781
Accession # AAA67061
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample

Human TGF-beta RIII Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Detection Reagent: Human TGF‑ beta  RIII Biotinylated Antibody (Catalog # BAF242)

Standard: Recombinant Human TGF-beta RIII Protein (Catalog # 242-R3)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RIII

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type I receptor is a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. The Type II receptor is also a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I and Type II receptors together form a heterodimeric signaling complex that is essential for the transduction of the anti-proliferative signals of TGF-beta. The Type III receptor is a transmembrane proteoglycan with a large extracellular domain and a 43 amino acid residue cytoplasmic domain. The cytoplasmic domain of the Type III receptor lacks an obvious signaling motif and the receptor may not be involved directly in signal transduction. The Type III receptor binds TGF-beta 2 with the highest affinity. Other TGF-beta isoforms also bind the Type III receptor, but with lower affinities. Cellular responsiveness to TGF-beta 2 appears to be dependent on the presence of the Type III receptor which can interact with the signaling receptor complex. In addition to the transmembrane Type III receptor, a soluble form of the receptor is secreted by some cell types. The physiological role of this soluble receptor remains to be determined. The recombinant TGF-beta soluble receptor Type III binds the TGF-beta isoforms differentially in solution and exhibits TGF-beta antagonistic activities in vitro.

Long Name
Transforming Growth Factor beta Receptor III
Entrez Gene IDs
7049 (Human); 21814 (Mouse); 29610 (Rat)
Alternate Names
betaglycan proteoglycan; Betaglycan; BGCAN; TBRIII; TGF-beta receptor type 3; TGF-beta receptor type III; TGF-beta RIII; TGFbetaRIII; TGFBR3; TGF-bRIII; TGFR-3; Transforming growth factor beta receptor III; transforming growth factor beta receptor type 3; transforming growth factor, beta receptor III (betaglycan, 300kDa); transforming growth factor, beta receptor III

Product Datasheets

FAQs

  1. What epitope does this antibody detect?

    • We have not done epitope mapping, but the antibody was raised against the extracellular domain of recombinant human TGF -beta RIII, Gly 21-Asp781, accession # AAA67061.  The antibody will react with the antigen in this region.

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human TGF-beta  RIII Antibody

Average Rating: 3 (Based on 2 Reviews)

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Human TGF-beta  RIII Antibody
By Anonymous on 12/06/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma


Human TGF-beta  RIII Antibody
By Anonymous on 11/08/2018
Application: ELISA Sample Tested: Plasma Species: Human