Human Thioredoxin-1 Antibody

Catalog # Availability Size / Price Qty

SAVE +FREE SHIPPING with ACADEMIA23 (See Promo Details)

Detection of Human Thioredoxin‑1 by Western Blot.
2 Images
Product Details
Supplemental Products

Human Thioredoxin-1 Antibody Summary

Species Reactivity
Detects human Thioredoxin‑1 in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 763629
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Thioredoxin‑1
Accession # P10599
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Thioredoxin-1 antibody by Western Blot. View Larger

Detection of Human Thioredoxin‑1 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Thioredoxin-1 Monoclonal Antibody (Catalog # MAB1970) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Thioredoxin-1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Thioredoxin-1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Thioredoxin‑1 in HeLa Human Cell Line. Thioredoxin-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Thioredoxin-1 Monoclonal Antibody (Catalog # MAB1970) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thioredoxin-1

Thioredoxins (Trxs) are a group of small ubiquitous proteins in all living cells that are key regulators of cellular redox balance (1, 2). The mammalian Trx family has three members. The Trx-1, which is a secreted and cellular protein, the mitochondria-specific Trx-2, and the Trx-like cytosolic protein p32TrxL (3-5). The active site of mammalian Trxs contains two cysteines in the conserved sequence -Y-C-G-P-C-K-. In Trx-1 the conserved cysteine residues are in positions 32 and 35, respectively. Trxs exist either in a reduced or in an oxidized state when the two cysteines at the active site form an intramolecular disulfide bridge. NADPH and the flavoprotein thioredoxin reductase can convert the oxidized Trx into the reduced Trx. Trx-1 is the only extracellular occurring thioredoxin, and is secreted by lymphocytes, hepatocytes, fibroblasts, and several tumor cells. Plasma concentrations of Trx-1 are up to 6 nM (6). In cells, Trx-1 is localized predominantly in the cytoplasm. Small amounts have been detected in the nucleus and in association with the outside surface of the cells. Expression of Trx-1 is increased under various stress conditions such as hypoxia, elevated hydrogen peroxide concentrations, photochemical oxidative stress, and viral and bacterial infections. Biological functions of Trx-1 include growth factor activity, antioxidant properties, a cofactor that provides reducing equivalents, and transcriptional regulation (1, 2). The synovial tissue of rheumatoid arthritis patients produces increased levels of Trx-1 under oxidative stress conditions, and a correlation exists between the plasma levels of Trx-1 and the severity of the disease, making Trx-1 a biomarker for this pathological condition (7, 8).

  1. Holmgren, A. (1985) Annu. Rev. Biochem. 54:237.
  2. Powis, G. and W.R. Monfort (2001) Annu. Rev. Pharm. Toxicol. 41:269.
  3. Deiss, L.P. and A. Kimchi (1991) Science 252:117.
  4. Spyrou, G. et al. (1997) J. Biol. Chem. 272:2936.
  5. Miranda-Vizuete, A. et al. (1998) Biochem. Biophys. Res. Commun. 243:284.
  6. Nakamura, H. et al. (1997) Annu. Rev. Immunol. 15:147.
  7. Mourice, M.M. et al. (1999) Arthritis Rheum. 42:2430.
  8. Jikimoto, T. et al. (2001) Mol. Immunol. 38:765.
Entrez Gene IDs
7295 (Human)
Alternate Names
Thioredoxin1; Thioredoxin-1; TRDX;TRX;TRX1;Trx80;TXN; Trx1; TXN; TXN1

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Thioredoxin-1 Antibody

There are currently no reviews for this product. Be the first to review Human Thioredoxin-1 Antibody and earn rewards!

Have you used Human Thioredoxin-1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review