Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 541213
View all THSD1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human THSD1 isoform 2
Glu25-Ile361
Accession # NP_954872

Specificity

Detects human TSHD1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human THSD1 Antibody

Detection of THSD1 antibody in HUVEC Human Cells antibody by Flow Cytometry.

Detection of THSD1 in HUVEC Human Cells by Flow Cytometry.

HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human THSD1 Monoclonal Antibody (Catalog # MAB5178, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).

Applications for Human THSD1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HUVEC human umbilical vein endothelial cells

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: THSD1

THSD1 (thrombospondin type 1 domain-containing protein 1; also TMTSP) is a 90‑100 kDa type I transmembrane protein that is reminiscent of Unc5h proteins. It is expressed on embryonic endothelial and hematopoietic stem cells, and may be involved in cell-cell interactions. Mature human THSD1 contains a 388 aa extracellular domain (ECD) (aa 25‑412) and a 418 aa cytoplasmic region (aa 434‑851). The ECD possesses three Ig-like domains (aa 156‑316) and one TSP type-1 domain (aa 340‑393). There is one potential alternate start site at Met380, and two splice variants, one of which shows a deletion of aa 341‑393, and a second that shows a 36 aa substitution for aa 394‑851, generating a soluble form. Over aa 25‑361, human THSD1 is 76% aa identical to mouse THSD1.

Long Name

Thrombospondin, Type I, Domain Containing 1

Alternate Names

TMTSP

Entrez Gene IDs

55901 (Human); 56229 (Mouse); 364630 (Rat)

Gene Symbol

THSD1

UniProt

NP_954872

Additional THSD1 Products

Product Documents for Human THSD1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human THSD1 Antibody

For research use only

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