Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2627A
View all Thymidine Kinase 1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived human Thymidine Kinase 1
Ser2-Asn234
Accession # P04183

Specificity

Detects human Thymidine Kinase 1 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Thymidine Kinase 1 Antibody

Detection of Human Thymidine Kinase 1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Thymidine Kinase 1 Monoclonal Antibody (Catalog # MAB81801) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Thymidine Kinase 1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human Thymidine Kinase 1 by Simple WesternTM.

Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, HeLa human cervical epithelial carcinoma cell line and SK‑BR‑3 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Thymidine Kinase 1 at approximately 32 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Thymidine Kinase 1 Monoclonal Antibody (Catalog # MAB81801). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Thymidine Kinase 1 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: A431 human epithelial carcinoma cell line, HeLa human cervical epithelial carcinoma cell line and SK‑BR‑3 human breast cancer cell line

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thymidine Kinase 1

Thymidine kinases are 2'-deoxythymidine kinases that phosphorylate deoxythymidine and generate deoxythymidine 5’-phosphate (1). Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Two forms of thymidine kinase are present in mammalian cells, TK1 and TK2. TK1 is cell cycle-dependent and is present in the cytoplasm only in anticipation of cell division (2, 3); whereas TK2 is located in mitochondria and is cell cycle-independent (4). TK1 is synthesized by the cell during the S phase of cell division and is degraded after cell division is completed (5). TK1 normally occurs in tissue as a dimer and can be converted to more active tetrameric form by ATP binding (6). TK1 is feedback inhibited by thymidine triphosphate, the product of the further phosphorylation of thymidine (7). Because tumor cells replicate much more frequently than normal cells and requires faster DNA synthesis and higher TK1 activity, TK1 is a cancer maker especially for hematologic malignancies (8, 9). In clinical chemistry TK1 is used as a proliferation marker in the diagnosis, control of treatment and follow-up of malignant disease (10). In addition, thymidine kinase is required for the action of many antiviral drugs (11). The enzymatic activity of recombinant human TK1 is measured using a phosphatase-coupled method (12).

References

  1. Wintersberger, E. (1997) Biochem. Soc. Trans. 25:303.
  2. Littelfield, J.W. (1966) Biochim. Biophys. Acta 114:398.
  3. Flemington, E, et al. (1987) Gene 52:267.
  4. Berk, A.J. et al. (1973) Arch. Biochem. Biophys. 154:563.
  5. Zhu, C. et al. (2006) Nucleosides Nucleotides Nucleic Acids 25:1185.
  6. Munch-Petersen, B. et al. (1993) J. Biol. Chem. 268:15621.
  7. Mikkelsen, N. E. et al. (2003). Biochemistry 42:5706.
  8. Doi S, et al. (1990) Nagoya J. Med. Sci. 52:19.
  9. Ellims PH, et al. (1981). Cancer Res. 41: 691.
  10. Lin TS, et al. (1976) J. Med. Chem.19:495.
  11. Baba M, et al. (1987). Biochem. Biophys. Res. Commun. 142:128.
  12. Wu, Z.L. (2011) PLoS One 6:e23172.

Alternate Names

TK1

Entrez Gene IDs

7083 (Human); 21877 (Mouse)

Gene Symbol

TK1

UniProt

P04183

Additional Thymidine Kinase 1 Products

Product Documents for Human Thymidine Kinase 1 Antibody

Certificate of Analysis

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Product Specific Notices for Human Thymidine Kinase 1 Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Intracellular Kinases

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