Human Tie-2 Antibody

(20 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Tie-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse Tie-2 is observed and less than 1% cross-reactivity with recombinant human Tie-1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Tie‑2
    Ala23-Lys745
    Accession # Q02763
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 5-10 µg/mL of this antibody will block 50% of the binding of 40 ng/mL of biotinylated Recombinant Human Angiopoietin-2 (Catalog # BT623) to immobilized Recombinant Human Tie-2 Fc Chimera (Catalog # 313-TI) coated at 4 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Tie‑2 by Western Blot. Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Tie‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF313) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Tie‑2 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Tie‑2 in Human Placenta. Tie‑2 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human Tie‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF313) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Tie‑2 by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for Tie‑2 at approximately 161 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Tie‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF313) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tie-2

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), which bind Tie-2 with high-affinity have been identified. Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.

  • References:
    1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
    2. Takakura, N. et al. (1998) Immunity 9:677.
    3. Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.
  • Long Name:
    Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
  • Entrez Gene IDs:
    7010 (Human); 21687 (Mouse); 30747 (Zebrafish)
  • Alternate Names:
    angiopoietin-1 receptor; CD202b antigen; CD202b; EC 2.7.10; EC 2.7.10.1; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; TEK tyrosine kinase, endothelial; TEK; Tie2; Tie-2; TIE2CD202b; Tunica interna endothelial cell kinase; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; venous malformations, multiple cutaneous and mucosal; VMCM; VMCM1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Structural basis of Tie2 activation and Tie2/Tie1 heterodimerization
    Authors: VM Leppänen, P Saharinen, K Alitalo
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Endothelial destabilization by angiopoietin-2 via integrin beta1 activation.
    Authors: Hakanpaa L, Sipila T, Leppanen V, Gautam P, Nurmi H, Jacquemet G, Eklund L, Ivaska J, Alitalo K, Saharinen P
    Nat Commun, 2015;6(0):5962.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  3. slanDCs selectively accumulate in carcinoma-draining lymph nodes and marginate metastatic cells.
    Authors: Vermi W, Micheletti A, Lonardi S, Costantini C, Calzetti F, Nascimbeni R, Bugatti M, Codazzi M, Pinter P, Schakel K, Tamassia N, Cassatella M
    Nat Commun, 2014;5(0):3029.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Chromatin structure of two genomic sites for targeted transgene integration in induced pluripotent stem cells and hematopoietic stem cells.
    Authors: van Rensburg, R, Beyer, I, Yao, X-Y, Wang, H, Denisenko, O, Li, Z-Y, Russell, D W, Miller, D G, Gregory, P, Holmes, M, Bomsztyk, K, Lieber, A
    Gene Ther, 2013;20(2):201-14.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  5. Exhaustion of nucleus pulposus progenitor cells with ageing and degeneration of the intervertebral disc.
    Authors: Sakai, Daisuke, Nakamura, Yoshihik, Nakai, Tomoko, Mishima, Taishi, Kato, Shunichi, Grad, Sibylle, Alini, Mauro, Risbud, Makarand, Chan, Danny, Cheah, Kathryn, Yamamura, Ken-ichi, Masuda, Koichi, Okano, Hideyuki, Ando, Kiyoshi, Mochida, Joji
    Nat Commun, 2012;3(0):1264.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Opposing effects of the angiopoietins on the thrombin-induced permeability of human pulmonary microvascular endothelial cells.
    Authors: van der Heijden M, van Nieuw Amerongen GP, van Bezu J, Paul MA, Groeneveld AB, van Hinsbergh VW
    PLoS ONE, 2011;6(8):e23448.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  7. VEGF-DdeltaNdeltaC mediated angiogenesis in skeletal muscles of diabetic WHHL rabbits.
    Authors: Roy H, Bhardwaj S, Babu M, Lahteenvuo JE, Yla-Herttuala S
    Eur. J. Clin. Invest., 2010;40(5):422-32.
    Species: Rabbit
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
    Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
    Eur. J. Haematol., 2010;84(3):239-51.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  9. Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells.
    Authors: Ricci-Vitiani L, Pallini R, Biffoni M, Todaro M, Invernici G, Cenci T, Maira G, Parati EA, Stassi G, Larocca LM, De Maria R
    Nature, 2010;468(7325):824-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. The angiogenic factor angiopoietin-1 is a proneurogenic peptide on subventricular zone stem/progenitor cells.
    Authors: Rosa AI, Goncalves J, Cortes L, Bernardino L, Malva JO, Agasse F
    J. Neurosci., 2010;30(13):4573-84.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  11. Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts.
    Authors: Saharinen P, Eklund L, Miettinen J, Wirkkala R, Anisimov A, Winderlich M, Nottebaum A, Vestweber D, Deutsch U, Koh GY, Olsen BR, Alitalo K
    Nat. Cell Biol., 2008;10(5):527-37.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  12. Interaction with human stromal cells enhances CXCR4 expression and engraftment of cord blood Lin(-)CD34(-) cells.
    Authors: Kobune M, Kawano Y, Takahashi S, Takada K, Murase K, Iyama S, Sato T, Takimoto R, Niitsu Y, Kato J
    Exp. Hematol., 2008;36(9):1121-31.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  13. Regulated proteolytic processing of Tie1 modulates ligand responsiveness of the receptor-tyrosine kinase Tie2.
    Authors: Marron MB, Singh H, Tahir TA, Kavumkal J, Kim HZ, Koh GY, Brindle NP
    J. Biol. Chem., 2007;282(42):30509-17.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  14. Interaction between Tie receptors modulates angiogenic activity of angiopoietin2 in endothelial progenitor cells.
    Authors: Kim KL, Shin IS, Kim JM, Choi JH, Byun J, Jeon ES, Suh W, Kim DK
    Cardiovasc. Res., 2006;72(3):394-402.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  15. Spatial and temporal distribution of Tie-1 and Tie-2 during very early development of the human placenta.
    Authors: Kayisli UA, Cayli S, Seval Y, Tertemiz F, Huppertz B, Demir R
    Placenta, 2006;27(6):648-59.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  16. Enhancement of angiogenic effectors through hypoxia-inducible factor in preterm primate lung in vivo.
    Authors: Asikainen TM, Waleh NS, Schneider BK, Clyman RI, White CW
    Am. J. Physiol. Lung Cell Mol. Physiol., 2006;291(4):L588-95.
    Species: Primate - Baboon
    Sample Type: Tissue Homogenates
    Application: WB
  17. Functional analysis of a mutant form of the receptor tyrosine kinase Tie2 causing venous malformations.
    Authors: Morris PN, Dunmore BJ, Tadros A, Marchuk DA, Darland DC, D&amp;amp;apos;Amore PA, Brindle NP
    J. Mol. Med., 2005;83(1):58-63.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  18. Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung.
    Authors: Asikainen TM, Schneider BK, Waleh NS, Clyman RI, Ho WB, Flippin LA, Gunzler V, White CW
    Proc. Natl. Acad. Sci. U.S.A., 2005;102(29):10212-7.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
  19. Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis.
    Authors: Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Almici C, Caramatti C, Mangoni L, Rizzoli V
    Blood, 2003;102(2):638-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  20. Evidence for heterotypic interaction between the receptor tyrosine kinases TIE-1 and TIE-2.
    Authors: Marron MB, Hughes DP, Edge MD, Forder CL, Brindle NP
    J. Biol. Chem., 2000;275(50):39741-6.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
Expand to show all 20 Citations
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