Home / TIMP-1 / Human TIMP-1 PE-conjugated Antibody

Human TIMP-1 PE-conjugated Antibody

Catalog #: IC970P
1 Citation Datasheet / COA / SDS

Discontinued Product

IC970P has been discontinued.
View all TIMP-1 products.
Detection of TIMP‑1 in NS0 Mouse Cell Line transfected with human TIMP-1 by Flow Cytometry.
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Human TIMP-1 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TIMP-1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) TIMP‑1 or rhTIMP‑2.
Source
Monoclonal Mouse IgG2B Clone # 63515
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIMP-1
Cys24-Ala207
Accession # P01033
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of TIMP-1 antibody in NS0 Mouse Cell Line transfected with human TIMP-1 antibody by Flow Cytometry. View Larger

Detection of TIMP‑1 in NS0 Mouse Cell Line transfected with human TIMP-1 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human TIMP-1 was stained with Mouse Anti-Human TIMP-1 PE-conjugated Monoclonal Antibody (Catalog # IC970P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: TIMP-1

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with Pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.

Long Name
Tissue Inhibitors of Metalloproteinases 1
Entrez Gene IDs
7076 (Human); 21857 (Mouse); 116510 (Rat)
Alternate Names
CLGI; Collagenase inhibitor; collagenase inhibitor); EPATIMP-1; EPO; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; TIMP1; TIMP-1; TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity; Tissue inhibitor of metalloproteinases 1

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Citation for Human TIMP-1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Cell expression of MMP-1 and TIMP-1 in co-cultures of human gingival fibroblasts and monocytes: the involvement of ICAM-1.
    Authors: Domeij H, Modeer T, Quezada HC, Yucel-Lindberg T
    Biochem. Biophys. Res. Commun., 2005-11-02;338(4):1825-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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