Human TNF RII/TNFRSF1B PE-conjugated Antibody

(9 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human TNF RII in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) 4‑1BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLymophotactin beta  R, rhNGF R, rhOPG, recombinant mouse (rm) OX40, rhRANK, rhTAJ, rhTNF RI, or rmTNF RII.
  • Source
    Monoclonal Mouse IgG2A Clone # 22235
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human TNF RII
    Extracellular domain
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TNF RII/TNFRSF1B in Human Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human TNF RII/TNFRSF1B PE‑conjugated Monoclonal Antibody (Catalog # FAB226P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TNF RII/TNFRSF1B
TNF RII is a type I transmembrane glycoprotein primarily expressed on hematopoietic cells and is designated TNFRSF1B. TNF RII is a high-affinity receptor for TNF‑ alpha and TNF-beta. A soluble form of the receptor generated by proteolytic cleavage functions as a TNF antagonist.
  • Long Name:
    Tumor Necrosis Factor Receptor II
  • Entrez Gene IDs:
    7133 (Human); 21938 (Mouse); 156767 (Rat)
  • Alternate Names:
    CD120b antigen; CD120b; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; soluble TNFR1B variant 1; TNF RII; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Applications
Sample Type
  1. TNFR2 increases the sensitivity of ligand-induced activation of the p38 MAPK and NF-kappaB pathways and signals TRAF2 protein degradation in macrophages.
    Authors: Ruspi G, Schmidt E, McCann F, Feldmann M, Williams R, Stoop A, Dean J
    Cell Signal, 2014;26(4):683-90.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
    Authors: Kalliolias GD, Gordon RA, Ivashkiv LB
    J. Immunol., 2010;185(11):7047-56.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes.
    Authors: Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis MA, Miossec P
    J. Immunol., 2009;182(0):3112-20.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha.
    Authors: Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA
    J. Immunol., 2007;179(7):4840-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Interaction between transmembrane TNF and TNFR1/2 mediates the activation of monocytes by contact with T cells.
    Authors: Rossol M, Meusch U, Pierer M, Kaltenhauser S, Hantzschel H, Hauschildt S, Wagner U
    J. Immunol., 2007;179(6):4239-48.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Phagocytosis of codeveloping megakaryocytic progenitors by dendritic cells in culture with thrombopoietin and tumor necrosis factor-alpha and its possible role in hemophagocytic syndrome.
    Authors: Saito K, Hirokawa M, Inaba K, Fukaya H, Kawabata Y, Komatsuda A, Yamashita J, Sawada K
    Blood, 2005;107(4):1366-74.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Codevelopment of dendritic cells along with erythroid differentiation from human CD34(+) cells by tumor necrosis factor-alpha.
    Authors: Fukaya H, Xiao W, Inaba K, Suzuki Y, Hirokawa M, Kawabata Y, Komatsuda A, Endo T, Kishimoto H, Takada G, Sawada K
    Exp. Hematol., 2004;32(5):450-60.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Tumor necrosis factor-alpha inhibits generation of glycophorin A+ cells by CD34+ cells.
    Authors: Xiao W, Koizumi K, Nishio M, Endo T, Osawa M, Fujimoto K, Sato I, Sakai T, Koike T, Sawada K
    Exp. Hematol., 2002;30(11):1238-47.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Inhibition of programmed cell death impairs in vitro vascular-like structure formation and reduces in vivo angiogenesis.
    Authors: Segura I, Serrano A, De Buitrago GG, Gonzalez MA, Abad JL, Claveria C, Gomez L, Bernad A, Martinez-A C, Riese HH
    FASEB J., 2002;16(8):833-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A PE-conjugated Antibody

Ctrl IC003P 13  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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