Human TNF RII/TNFRSF1B PE-conjugated Antibody

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Human TNF RII/TNFRSF1B PE-conjugated Antibody in Data
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Product Details
Citations (11)
Supplemental Products
Reviews (1)

Human TNF RII/TNFRSF1B PE-conjugated Antibody Summary

Species Reactivity
Detects human TNF RII in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) 4‑1BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLymophotactin beta  R, rhNGF R, rhOPG, recombinant mouse (rm) OX40, rhRANK, rhTAJ, rhTNF RI, or rmTNF RII.
Monoclonal Mouse IgG2A Clone # 22235
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human TNF RII
Extracellular domain
Supplied in a saline solution containing BSA and Sodium Azide.
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TNF RII/TNFRSF1B antibody in Human Granulocytes antibody by Flow Cytometry. View Larger

Detection of TNF RII/TNFRSF1B in Human Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human TNF RII/TNFRSF1B PE-conjugated Monoclonal Antibody (Catalog # FAB226P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TNF RII/TNFRSF1B

TNF RII is a type I transmembrane glycoprotein primarily expressed on hematopoietic cells and is designated TNFRSF1B. TNF RII is a high-affinity receptor for TNF‑ alpha and TNF-beta. A soluble form of the receptor generated by proteolytic cleavage functions as a TNF antagonist.

Long Name
Tumor Necrosis Factor Receptor II
Entrez Gene IDs
7133 (Human); 21938 (Mouse); 156767 (Rat); 102144224 (Cynomolgus Monkey)
Alternate Names
CD120b antigen; CD120b; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; soluble TNFR1B variant 1; TNF RII; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II

Product Datasheets

Citations for Human TNF RII/TNFRSF1B PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. TNFR2 ligation in human T regulatory cells enhances IL2-induced cell proliferation through the non-canonical NF-?B pathway
    Authors: J Wang, R Ferreira, W Lu, S Farrow, K Downes, L Jermutus, R Minter, RS Al-Lamki, JS Pober, JR Bradley
    Sci Rep, 2018;8(1):12079.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Memory Regulatory T cells Increase Only In Inflammatory Phase of Chronic Hepatitis B Infection and Related to Galectin-9/Tim-3 interaction
    Authors: CC Hu, WJ Jeng, YC Chen, JH Fang, CH Huang, W Teng, YC Hsieh, YC Lin, RN Chien, IS Sheen, CY Lin
    Sci Rep, 2017;7(1):15280.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. TNFR2 increases the sensitivity of ligand-induced activation of the p38 MAPK and NF-kappaB pathways and signals TRAF2 protein degradation in macrophages.
    Authors: Ruspi G, Schmidt E, McCann F, Feldmann M, Williams R, Stoop A, Dean J
    Cell Signal, 2014;26(4):683-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
    Authors: Kalliolias GD, Gordon RA, Ivashkiv LB
    J. Immunol., 2010;185(11):7047-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes.
    Authors: Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis MA, Miossec P
    J. Immunol., 2009;182(0):3112-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Interaction between transmembrane TNF and TNFR1/2 mediates the activation of monocytes by contact with T cells.
    Authors: Rossol M, Meusch U, Pierer M, Kaltenhauser S, Hantzschel H, Hauschildt S, Wagner U
    J. Immunol., 2007;179(6):4239-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha.
    Authors: Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA
    J. Immunol., 2007;179(7):4840-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Phagocytosis of codeveloping megakaryocytic progenitors by dendritic cells in culture with thrombopoietin and tumor necrosis factor-alpha and its possible role in hemophagocytic syndrome.
    Authors: Saito K, Hirokawa M, Inaba K, Fukaya H, Kawabata Y, Komatsuda A, Yamashita J, Sawada K
    Blood, 2005;107(4):1366-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Codevelopment of dendritic cells along with erythroid differentiation from human CD34(+) cells by tumor necrosis factor-alpha.
    Authors: Fukaya H, Xiao W, Inaba K, Suzuki Y, Hirokawa M, Kawabata Y, Komatsuda A, Endo T, Kishimoto H, Takada G, Sawada K
    Exp. Hematol., 2004;32(5):450-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Tumor necrosis factor-alpha inhibits generation of glycophorin A+ cells by CD34+ cells.
    Authors: Xiao W, Koizumi K, Nishio M, Endo T, Osawa M, Fujimoto K, Sato I, Sakai T, Koike T, Sawada K
    Exp. Hematol., 2002;30(11):1238-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Inhibition of programmed cell death impairs in vitro vascular-like structure formation and reduces in vivo angiogenesis.
    Authors: Segura I, 2019, Serrano A, De Buitrago GG, Gonzalez MA, Abad JL, Claveria C, Gomez L, Bernad A, Martinez-A C, Riese HH
    7096, 2002;16(8):833-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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Human TNF RII/TNFRSF1B PE-conjugated Antibody
By Anonymous on 06/07/2018
Application: ICC/IF Sample Tested: pyoderma gangrenosum,Skin tissue Species: Human