Human Total MMP-7 Quantikine ELISA Kit

(20 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (25 uL), Serum (25 uL), Heparin Plasma (25 uL), Saliva (25 uL), Urine (50 uL)
  • Sensitivity
    0.094 ng/mL
  • Assay Range
    0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine)
  • Specificity
    Natural and recombinant human MMP-7
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human MMP-7 Immunoassay is a 4.5 hour solid phase ELISA designed to measure MMP-7 in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human MMP-7 and antibodies raised against the recombinant factor. The Quantikine kit detected both pro- and active forms of recombinant human MMP-7. Natural human MMP-7 showed dose-response curves that were parallel to the standard curves obtained using the kit standards, indicating that this kit can be used to determine relative levels of natural human MMP-7.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.0640.1680.2170.0540.1980.309


The recovery of MMP-7 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 96-108
Urine (n=2) 99 89-109
To assess the linearity of the assay, samples spiked with or containing high concentrations of MMP-7 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line
Human Total MMP-7 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MMP-7

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

    • Long Name
      Matrix Metalloproteinase 7
    • Entrez Gene IDs
      4316 (Human); 17393 (Mouse);
    • Alternate Names
      EC 3.4.24; EC; matrilysin; Matrin; matrix metallopeptidase 7 (matrilysin, uterine); matrix metalloproteinase 7 (matrilysin, uterine); Matrix metalloproteinase-7; MMP7; MMP-7; MPSL1; Pump-1 protease; PUMP1; PUMP-1; uterine matrilysin; Uterine metalloproteinase;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 20
    Filter your results:

    Sample Type
    1. Suppression of Aurora-A-FLJ10540 signaling axis prohibits the malignant state of head and neck cancer.
      Authors: Chen, Chang-Ha, Chang, Alice Y, Li, Shau-Hsu, Tsai, Hsin-Tin, Shiu, Li-Yen, Su, Li-Jen, Wang, Wen-Lung, Chiu, Tai-Jen, Luo, Sheng-De, Huang, Tai-Lin, Chien, Chih-Yen
      Mol Cancer, 2015;14(0):83.
      Species: Human
      Sample Type: Cell Culture Supernates
    2. Role of SIRT1 in regulation of epithelial-to-mesenchymal transition in oral squamous cell carcinoma metastasis.
      Authors: Chen I, Chiang W, Huang H, Chen P, Shen Y, Chiang H
      Mol Cancer, 2014;13(0):254.
      Species: Human
      Sample Type: cell culture supernate
    3. Expression of CD24 in cholangiocarcinoma cells is associated with disease progression and reduced patient survival.
      Authors: Keeratichamroen S, Leelawat K, Thongtawee T
      Int. J. Oncol., 2011;39(4):873-81.
    4. Stat3 and MMP7 contribute to pancreatic ductal adenocarcinoma initiation and progression.
      Authors: Fukuda A, Wang SC, Morris JP, Folias AE, Liou A, Kim GE, Akira S, Boucher KM, Firpo MA, Mulvihill SJ, Hebrok M
      Cancer Cell, 2011;19(4):441-55.
      Species: Human
      Sample Type: Serum
    5. Urinary matrix metalloproteinase-7 level is associated with the presence of metastasis in bladder cancer.
      Authors: Szarvas T, Singer BB, Becker M, vom Dorp F, Jager T, Szendroi A, Riesz P, Romics I, Rubben H, Ergun S
      BJU Int., 2011;107(7):1069-73.
    6. Validation of circulating MMP-7 level as an independent prognostic marker of poor survival in urinary bladder cancer.
      Authors: Szarvas T, Jager T, Becker M, Tschirdewahn S, Niedworok C, Kovalszky I, Rubben H, Ergun S, vom Dorp F
      Pathol. Oncol. Res., 2011;17(2):325-32.
      Species: Human
      Sample Type: Plasma
    7. Elevated serum matrix metalloproteinase 7 levels predict poor prognosis after radical prostatectomy.
      Authors: Szarvas T, Becker M, vom Dorp F, Meschede J, Scherag A, Bankfalvi A, Reis H, Schmid KW, Romics I, Rubben H, Ergun S
      Int. J. Cancer, 2011;128(6):1486-92.
      Species: Human
      Sample Type: Serum
    8. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
      Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
      Eur. J. Haematol., 2010;84(3):239-51.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. Elevated serum matrix metalloproteinase-3 and -7 in H. pylori-related gastric cancer can be biomarkers correlating with a poor survival.
      Authors: Yeh YC, Sheu BS, Cheng HC, Wang YL, Yang HB, Wu JJ
      Dig. Dis. Sci., 2010;55(6):1649-57.
      Species: Human
      Sample Type: Serum
    10. Matrix metalloproteinase-7 as a marker of metastasis and predictor of poor survival in bladder cancer.
      Authors: Szarvas T, Becker M, vom Dorp F, Gethmann C, Totsch M, Bankfalvi A, Schmid KW, Romics I, Rubben H, Ergun S
      Cancer Sci., 2010;101(5):1300-8.
      Species: Human
      Sample Type: Serum
    11. Gingival fibroblast inhibits MMP-7: evaluation in an ex vivo aorta model.
      Authors: Gogly B, Fournier B, Couty L, Naveau A, Brasselet C, Durand E, Coulomb B, Lafont A
      J. Mol. Cell. Cardiol., 2009;47(2):296-303.
      Species: Rabbit
      Sample Type: Cell Culture Supernates
    12. Fibulin-5 suppresses lung cancer invasion by inhibiting matrix metalloproteinase-7 expression.
      Authors: Yue W, Sun Q, Landreneau R, Wu C, Siegfried JM, Yu J, Zhang L
      Cancer Res., 2009;69(15):6339-46.
      Species: Human
      Sample Type: Cells Supernates
    13. MMP1 and MMP7 as potential peripheral blood biomarkers in idiopathic pulmonary fibrosis.
      Authors: Rosas IO, Richards TJ, Konishi K, Zhang Y, Gibson K, Lokshin AE, Lindell KO, Cisneros J, Macdonald SD, Pardo A, Sciurba F, Dauber J, Selman M, Gochuico BR, Kaminski N
      PLoS Med., 2008;5(4):e93.
      Species: Human
      Sample Type: Serum
    14. Circulating matrix metalloproteinase-7: an early or metastatic marker for renal cell carcinoma?
      Authors: Jung K, Ramankulov A, Schrader M, Miller K, Lein M
      Clin. Chem., 2008;54(11):1927-9.
      Species: Human
      Sample Type: Plasma
    15. Generation of endostatin by matrix metalloproteinase and cathepsin from human limbocorneal epithelial cells cultivated on amniotic membrane.
      Authors: Ma DH, Yao JY, Kuo MT, See LC, Lin KY, Chen SC, Chen JK, Chao AS, Wang SF, Lin KK
      Invest. Ophthalmol. Vis. Sci., 2007;48(2):644-51.
      Species: Human
      Sample Type: Cell Culture Supernates
    16. Is metalloproteinase-7 specific for idiopathic pulmonary fibrosis?
      Authors: Huh JW, Kim DS, Oh YM, Shim TS, Lim CM, Lee SD, Koh Y, Kim WS, Kim WD, Kim KR
      Chest, 2007;133(5):1101-6.
      Species: Human
      Sample Type: BALF
    17. Serum biobank certification and the establishment of quality controls for biological fluids: examples of serum biomarker stability after temperature variation.
      Authors: Chaigneau C, Cabioch T, Beaumont K, Betsou F
      Clin. Chem. Lab. Med., 2007;45(10):1390-5.
      Species: Human
      Sample Type: Serum
    18. An integrated clinical-genomics approach identifies a candidate multi-analyte blood test for serous ovarian carcinoma.
      Authors: Meinhold-Heerlein I, Bauerschlag D, Zhou Y, Sapinoso LM, Ching K, Frierson H, Brautigam K, Sehouli J, Stickeler E, Konsgen D, Hilpert F, von Kaisenberg CS, Pfisterer J, Bauknecht T, Jonat W, Arnold N, Hampton GM
      Clin. Cancer Res., 2007;13(2):458-66.
      Species: Human
      Sample Type: Serum
    19. Advanced glycation end products decrease mesangial cell MMP-7: a role in matrix accumulation in diabetic nephropathy?
      Authors: McLennan SV, Kelly DJ, Schache M, Waltham M, Dy V, Langham RG, Yue DK, Gilbert RE
      Kidney Int., 2007;72(4):481-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    20. Determination of matrilysin activity in gastrointestinal neoplasia.
      Authors: Hawinkels LJ, Verspaget HW, van den Berg M, Hanemaaijer R, Sier CF
      Eur. J. Clin. Invest., 2007;37(7):598-9.
      Species: Human
      Sample Type: Tissue Homogenates
    Expand to show all
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 837 for Human Total MMP-7

    Ctrl QC128
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....
    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine Wash Buffer 1

    ELISA WA126 5
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....

    Have you used Human Total MMP-7 Quantikine ELISA Kit?

    Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    Order Details

    Contact Information

    Certificate of Analysis Lookup

    The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

    Certificate of Analysis Lookup

    Research Areas of Interest
    R&D Systems Guarantee


    Customer Information