Human Total MMP-7 Quantikine ELISA Kit

  (24 citations)     
Datasheet / CoA / SDS
Product Details
Assay Procedure
Citations (24)
FAQs
Supplemental Products
Reviews
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (25 uL), Serum (25 uL), Heparin Plasma (25 uL), Saliva (25 uL), Urine (50 uL)
  • Sensitivity
    0.094 ng/mL
  • Assay Range
    0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine)
  • Specificity
    Natural and recombinant human MMP-7
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC128 , Quantikine Immunoassay Control Set 837 for Human Total MMP-7 - Please Inquire
Product Summary
The Quantikine Human MMP-7 Immunoassay is a 4.5 hour solid phase ELISA designed to measure MMP-7 in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human MMP-7 and antibodies raised against the recombinant factor. The Quantikine kit detected both pro- and active forms of recombinant human MMP-7. Natural human MMP-7 showed dose-response curves that were parallel to the standard curves obtained using the kit standards, indicating that this kit can be used to determine relative levels of natural human MMP-7.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 1.26 4.58 6.29 1.23 4.82 6.76
Standard Deviation 0.064 0.168 0.217 0.054 0.198 0.309
CV% 5.1 3.7 3.4 4.4 4.1 4.6

Recovery

The recovery of MMP-7 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 96-108
Urine (n=2) 99 89-109
Linearity
To assess the linearity of the assay, samples spiked with or containing high concentrations of MMP-7 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line
 MMP-7 [HRP]
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MMP-7

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

  • Long Name:
    Matrix Metalloproteinase 7
  • Entrez Gene IDs:
    4316 (Human); 17393 (Mouse)
  • Alternate Names:
    EC 3.4.24; EC 3.4.24.23; Matrilysin; matrin; matrix metallopeptidase 7 (matrilysin, uterine); matrix metalloproteinase 7 (matrilysin, uterine); Matrix metalloproteinase-7; MMP7; MMP-7; MPSL1; Pump-1 protease; PUMP1; PUMP-1; uterine matrilysin; Uterine metalloproteinase
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
Filter your results:

Species
Sample Type
  1. Serum Matrix Metalloproteinase-7 Level is Associated with Fibrosis and Renal Survival in Patients with IgA Nephropathy
    Authors: J Zhang, P Ren, Y Wang, S Feng, C Wang, X Shen, C Weng, X Lang, Z Chen, H Jiang, J Chen
    Kidney Blood Press. Res., 2017;42(3):541-552.
    Species: Human
    Sample Type: Serum
  2. The interdependencies of viral load, the innate immune response, and clinical outcome in children presenting to the emergency department with respiratory syncytial virus-associated bronchiolitis
    Authors: FA Piedra, M Mei, V Avadhanula, R Mehta, L Aideyan, RP Garofalo, PA Piedra
    PLoS ONE, 2017;12(3):e0172953.
    Species: Human
    Sample Type: Nasal Lavage Fluid
  3. Mucin 4 and matrix metalloproteinase 7 as novel salivary biomarkers for periodontitis
    Authors: Anna Lundmark
    J. Clin. Periodontol, 2016;0(0):.
    Species: Human
    Sample Type: Saliva
  4. Concomitant elevations of MMP-9, NGAL, proMMP-9/NGAL and neutrophil elastase in serum of smokers with chronic obstructive pulmonary disease
    J. Cell. Mol. Med, 2016;0(0):.
    Species: Human
    Sample Type: Serum
  5. Suppression of Aurora-A-FLJ10540 signaling axis prohibits the malignant state of head and neck cancer.
    Authors: Chen, Chang-Ha, Chang, Alice Y, Li, Shau-Hsu, Tsai, Hsin-Tin, Shiu, Li-Yen, Su, Li-Jen, Wang, Wen-Lung, Chiu, Tai-Jen, Luo, Sheng-De, Huang, Tai-Lin, Chien, Chih-Yen
    Mol Cancer, 2015;14(0):83.
    Species: Human
    Sample Type: Cell Culture Supernates
  6. Role of SIRT1 in regulation of epithelial-to-mesenchymal transition in oral squamous cell carcinoma metastasis.
    Authors: Chen I, Chiang W, Huang H, Chen P, Shen Y, Chiang H
    Mol Cancer, 2014;13(0):254.
    Species: Human
    Sample Type: Cell Culture Supernates
  7. Elevated serum matrix metalloproteinase 7 levels predict poor prognosis after radical prostatectomy.
    Authors: Szarvas T, Becker M, vom Dorp F, Meschede J, Scherag A, Bankfalvi A, Reis H, Schmid KW, Romics I, Rubben H, Ergun S
    Int. J. Cancer, 2011;128(6):1486-92.
    Species: Human
    Sample Type: Serum
  8. Urinary matrix metalloproteinase-7 level is associated with the presence of metastasis in bladder cancer.
    Authors: Szarvas T, Singer BB, Becker M, vom Dorp F, Jager T, Szendroi A, Riesz P, Romics I, Rubben H, Ergun S
    BJU Int., 2011;107(7):1069-73.
  9. Expression of CD24 in cholangiocarcinoma cells is associated with disease progression and reduced patient survival.
    Authors: Keeratichamroen S, Leelawat K, Thongtawee T
    Int. J. Oncol., 2011;39(4):873-81.
  10. Stat3 and MMP7 contribute to pancreatic ductal adenocarcinoma initiation and progression.
    Authors: Fukuda A, Wang SC, Morris JP, Folias AE, Liou A, Kim GE, Akira S, Boucher KM, Firpo MA, Mulvihill SJ, Hebrok M
    Cancer Cell, 2011;19(4):441-55.
    Species: Human
    Sample Type: Serum
  11. Validation of circulating MMP-7 level as an independent prognostic marker of poor survival in urinary bladder cancer.
    Authors: Szarvas T, Jager T, Becker M, Tschirdewahn S, Niedworok C, Kovalszky I, Rubben H, Ergun S, vom Dorp F
    Pathol. Oncol. Res., 2011;17(2):325-32.
    Species: Human
    Sample Type: Plasma
  12. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
    Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
    Eur. J. Haematol., 2010;84(3):239-51.
    Species: Human
    Sample Type: Cell Culture Supernates
  13. Matrix metalloproteinase-7 as a marker of metastasis and predictor of poor survival in bladder cancer.
    Authors: Szarvas T, Becker M, vom Dorp F, Gethmann C, Totsch M, Bankfalvi A, Schmid KW, Romics I, Rubben H, Ergun S
    Cancer Sci., 2010;101(5):1300-8.
    Species: Human
    Sample Type: Serum
  14. Elevated serum matrix metalloproteinase-3 and -7 in H. pylori-related gastric cancer can be biomarkers correlating with a poor survival.
    Authors: Yeh YC, Sheu BS, Cheng HC, Wang YL, Yang HB, Wu JJ
    Dig. Dis. Sci., 2010;55(6):1649-57.
    Species: Human
    Sample Type: Serum
  15. Fibulin-5 suppresses lung cancer invasion by inhibiting matrix metalloproteinase-7 expression.
    Authors: Yue W, Sun Q, Landreneau R, Wu C, Siegfried JM, Yu J, Zhang L
    Cancer Res., 2009;69(15):6339-46.
    Species: Human
    Sample Type: Cell Culture Supernates
  16. Gingival fibroblast inhibits MMP-7: evaluation in an ex vivo aorta model.
    Authors: Gogly B, Fournier B, Couty L, Naveau A, Brasselet C, Durand E, Coulomb B, Lafont A
    J. Mol. Cell. Cardiol., 2009;47(2):296-303.
    Species: Rabbit
    Sample Type: Cell Culture Supernates
  17. MMP1 and MMP7 as potential peripheral blood biomarkers in idiopathic pulmonary fibrosis.
    Authors: Rosas IO, Richards TJ, Konishi K, Zhang Y, Gibson K, Lokshin AE, Lindell KO, Cisneros J, Macdonald SD, Pardo A, Sciurba F, Dauber J, Selman M, Gochuico BR, Kaminski N
    PLoS Med., 2008;5(4):e93.
    Species: Human
    Sample Type: Serum
  18. Circulating matrix metalloproteinase-7: an early or metastatic marker for renal cell carcinoma?
    Authors: Jung K, Ramankulov A, Schrader M, Miller K, Lein M
    Clin. Chem., 2008;54(11):1927-9.
    Species: Human
    Sample Type: Plasma
  19. An integrated clinical-genomics approach identifies a candidate multi-analyte blood test for serous ovarian carcinoma.
    Authors: Meinhold-Heerlein I, Bauerschlag D, Zhou Y, Sapinoso LM, Ching K, Frierson H, Brautigam K, Sehouli J, Stickeler E, Konsgen D, Hilpert F, von Kaisenberg CS, Pfisterer J, Bauknecht T, Jonat W, Arnold N, Hampton GM
    Clin. Cancer Res., 2007;13(2):458-66.
    Species: Human
    Sample Type: Serum
  20. Advanced glycation end products decrease mesangial cell MMP-7: a role in matrix accumulation in diabetic nephropathy?
    Authors: McLennan SV, Kelly DJ, Schache M, Waltham M, Dy V, Langham RG, Yue DK, Gilbert RE
    Kidney Int., 2007;72(4):481-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  21. Is metalloproteinase-7 specific for idiopathic pulmonary fibrosis?
    Authors: Huh JW, Kim DS, Oh YM, Shim TS, Lim CM, Lee SD, Koh Y, Kim WS, Kim WD, Kim KR
    Chest, 2007;133(5):1101-6.
    Species: Human
    Sample Type: BALF
  22. Determination of matrilysin activity in gastrointestinal neoplasia.
    Authors: Hawinkels LJ, Verspaget HW, van den Berg M, Hanemaaijer R, Sier CF
    Eur. J. Clin. Invest., 2007;37(7):598-9.
    Species: Human
    Sample Type: Tissue Homogenates
  23. Serum biobank certification and the establishment of quality controls for biological fluids: examples of serum biomarker stability after temperature variation.
    Authors: Chaigneau C, Cabioch T, Beaumont K, Betsou F
    Clin. Chem. Lab. Med., 2007;45(10):1390-5.
    Species: Human
    Sample Type: Serum
  24. Generation of endostatin by matrix metalloproteinase and cathepsin from human limbocorneal epithelial cells cultivated on amniotic membrane.
    Authors: Ma DH, Yao JY, Kuo MT, See LC, Lin KY, Chen SC, Chen JK, Chao AS, Wang SF, Lin KK
    Invest. Ophthalmol. Vis. Sci., 2007;48(2):644-51.
    Species: Human
    Sample Type: Cell Culture Supernates
Expand to show all Citations

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs
ELISA Controls
Description Application Cat# Citations Images  

Quantikine Immunoassay Control Set 837 for Human Total MMP-7

Ctrl QC128
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Supplemental ELISA Products
Description Application Cat# Citations Images  

Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human Total MMP-7 Quantikine ELISA Kit?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order
reload

Feedback

Customer Information
R&D Systems Guarantee