Human Total MMP-8 Quantikine ELISA Kit

R&D Systems | Catalog # DMP800B

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Tissue Lysates (50 µL), Serum (10 µL), EDTA Plasma (25 µL), Platelet-poor EDTA Plasma (50 µL), Heparin Plasma (25 µL), Platelet-poor Heparin Plasma (50 µL), Saliva (10 µL)

Sensitivity

0.058 ng/mL

Assay Range

0.156-10 ng/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Saliva)
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Product Summary for Human Total MMP-8 Quantikine ELISA Kit

The Quantikine® Human Total MMP-8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure total MMP-8 (pro- and active MMP-8) in cell culture supernates, tissue lysates, serum, plasma, platelet-poor plasma, and saliva. It contains NS0-expressed recombinant human MMP-8, and antibodies raised against the recombinant protein. Natural human MMP-8 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MMP-8.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human MMP-8.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva, Serum, Tissue Lysates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.764 2.38 4.55 0.836 2.41 5.07
Standard Deviation 0.049 0.070 0.299 0.055 0.171 0.270
CV% 6.4 2.9 6.6 6.6 7.1 5.3

Recovery for Human Total MMP-8 Quantikine ELISA Kit

The recovery of human Total MMP-8 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 98 93-103
EDTA Plasma (n=4) 92 86-99
Extraction Buffer (1X) (n=2) 84 83-85
Heparin Plasma (n=4) 92 86-99
Platelet-poor EDTA Plasma (n=4) 85 79-91
Platelet-poor Heparin Plasma (n=4) 84 78-89
Saliva (n=4) 96 86-111
Serum (n=4) 101 93-109

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Total MMP-8 in various matrices were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Human Total MMP-8 ELISA Linearity
Human Total MMP-8 ELISA Linearity

Scientific Data Images for Human Total MMP-8 Quantikine ELISA Kit

Human Total MMP-8 Standard Curve

Human Total MMP-8 Standard Curve

Human Total MMP-8 ELISA Standard Curve

Human Total MMP-8 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-8

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name

Matrix Metalloproteinase 8

Alternate Names

Collagenase 2, MMP8

Entrez Gene IDs

4317 (Human); 17394 (Mouse); 63849 (Rat)

Gene Symbol

MMP8

Additional MMP-8 Products

Product Documents for Human Total MMP-8 Quantikine ELISA Kit

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Total MMP-8 Quantikine ELISA Kit

For research use only

Citations for Human Total MMP-8 Quantikine ELISA Kit

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