Human Total MMP-8 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DMP800B
Control Products Available
Human Total MMP-8 ELISA Standard Curve
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Product Details
Citations (21)
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Human Total MMP-8 Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (25 uL), Platelet-poor EDTA Plasma (50 uL), Heparin Plasma (25 uL), Platelet-poor Heparin Plasma (50 uL), Saliva (10 uL)
Sensitivity
0.058 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Saliva)
Specificity
Natural and recombinant human MMP-8.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human Total MMP-8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure total MMP-8 (pro- and active MMP-8) in cell culture supernates, tissue lysates, serum, plasma, platelet-poor plasma, and saliva. It contains NS0-expressed recombinant human MMP-8, and antibodies raised against the recombinant protein. Natural human MMP-8 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MMP-8.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 0.764 2.38 4.55 0.836 2.41 5.07
Standard Deviation 0.049 0.07 0.299 0.055 0.171 0.27
CV% 6.4 2.9 6.6 6.6 7.1 5.3

Recovery

The recovery of human Total MMP-8 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 98 93-103
EDTA Plasma (n=4) 92 86-99
Extraction Buffer (1X) (n=2) 84 83-85
Heparin Plasma (n=4) 92 86-99
Platelet-poor EDTA Plasma (n=4) 85 79-91
Platelet-poor Heparin Plasma (n=4) 84 78-89
Saliva (n=4) 96 86-111
Serum (n=4) 101 93-109

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Total MMP-8 in various matrices were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human Total MMP-8 ELISA Linearity

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-8

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name:
Matrix Metalloproteinase 8
Entrez Gene IDs:
4317 (Human); 17394 (Mouse); 63849 (Rat)
Alternate Names:
CLG1HNC; Collagenase 2; EC 3.4.24; EC 3.4.24.34; matrix metallopeptidase 8 (neutrophil collagenase); matrix metalloproteinase 8 (neutrophil collagenase); Matrix metalloproteinase-8; MMP8; MMP-8; neutrophil collagenase; PMNL collagenase; PMNL-CL

Citations for Human Total MMP-8 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. MMP-8 and TIMP-1 are associated to periodontal inflammation in patients with rheumatoid arthritis under methotrexate immunosuppression - First results of a cross-sectional study
    Authors: G Schmalz, I Davarpanah, J Jäger, RF Mausberg, B Krohn-Grim, J Schmidt, R Haak, U Sack, D Ziebolz
    J Microbiol Immunol Infect, 2017;0(0):.
    Species: Human
    Sample Types: Serum
  2. The effect of type 2 diabetes mellitus and smoking on periodontal parameters and salivary matrix metalloproteinase-8 levels
    Authors: N Gupta, ND Gupta, S Garg, L Goyal, A Gupta, S Khan, S Moin
    J Oral Sci, 2016;58(1):1-6.
    Species: Human
    Sample Types: Saliva
  3. Relationship between Serum Levels of Metalloproteinase-8 and Tissue Inhibitor of Metalloproteinases-1 and Exercise Test Results in Postmenopausal Women
    Authors: J Mieczkowsk, E Rutkowska, J Mosiewicz, B Mosiewicz
    Dis. Markers, 2016;2016(0):7169531.
    Species: Human
    Sample Types: Serum
  4. Identification of Gingival Crevicular Fluid Sampling, Analytical Methods, and Oral Biomarkers for the Diagnosis and Monitoring of Periodontal Diseases: A Systematic Review
    Authors: Z Nazar Maje, K Philip, AM Alabsi, S Pushparaja, D Swaminatha
    Dis. Markers, 2016;2016(0):1804727.
    Species: Human
    Sample Types: Saliva
  5. Interleukin-6 and matrix metalloproteinase expression in the subretinal fluid during proliferative vitreoretinopathy: correlation with extent, duration of RRD and PVR grade.
    Authors: Symeonidis C, Papakonstantinou E, Androudi S, Tsaousis KT, Tsinopoulos I, Brazitikos P, Karakiulakis G, Diza E, Dimitrakos SA
    Cytokine, 2012;59(1):184-90.
    Species: Human
    Sample Types: Subretinal Fluid
  6. Validation and quality control of ELISAs for the use with human saliva samples.
    Authors: Jaedicke KM, Taylor JJ, Preshaw PM
    J. Immunol. Methods, 2012;377(1):62-5.
    Species: Human
    Sample Types: Saliva
  7. Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans.
    Authors: Aronis KN, Diakopoulos KN, Fiorenza CG
    Diabetologia, 2011;54(9):2358-67.
    Species: Human
    Sample Types: Serum
  8. Interleukin-6 and the matrix metalloproteinase response in the vitreous during proliferative vitreoretinopathy.
    Authors: Symeonidis C, Papakonstantinou E, Androudi S, Rotsos T, Diza E, Brazitikos P, Karakiulakis G, Dimitrakos SA
    Cytokine, 2011;54(2):212-7.
    Species: Human
    Sample Types: Vitreous Humor
  9. Increased levels of leukocyte-derived MMP-9 in patients with stable angina pectoris.
    Authors: Jonasson S, Lundberg A, Kalvegren H, Bergstrom I, Szymanowski A, Jonasson L
    PLoS ONE, 2011;6(4):e19340.
    Species: Human
    Sample Types: Serum
  10. Early Elevation of Matrix Metalloproteinase-8 and -9 in Pediatric ARDS Is Associated with an Increased Risk of Prolonged Mechanical Ventilation.
    Authors: Kong MY, Li Y, Oster R, Gaggar A, Clancy JP
    PLoS ONE, 2011;6(8):e22596.
    Species: Human
    Sample Types: Tissue Secretion
  11. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
    Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
    Eur. J. Haematol., 2010;84(3):239-51.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Serum matrix metalloproteinases in adult CF patients: Relation to pulmonary exacerbation.
    Authors: Roderfeld M, Rath T, Schulz R, Seeger W, Tschuschner A, Graf J, Roeb E
    J. Cyst. Fibros., 2009;8(5):338-47.
    Species: Human
    Sample Types: Serum
  13. Targeting a metalloprotease-PAR1 signaling system with cell-penetrating pepducins inhibits angiogenesis, ascites, and progression of ovarian cancer.
    Authors: Agarwal A, Covic L, Sevigny LM, Kaneider NC, Lazarides K, Azabdaftari G, Sharifi S, Kuliopulos A
    Mol. Cancer Ther., 2008;7(9):2746-57.
    Species: Human
    Sample Types: Ovarian Ascites Fluid
  14. Matrix metalloprotease-9 dysregulation in lower airway secretions of cystic fibrosis patients.
    Authors: Gaggar A, Li Y, Weathington N, Winkler M, Kong M, Jackson P, Blalock JE, Clancy JP
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L96-L104.
    Species: Human
    Sample Types: Sputum
  15. Changes in periodontal and rheumatological conditions after 2 years in patients with juvenile idiopathic arthritis.
    Authors: Miranda LA, Braga F, Fischer RG, Sztajnbok FR, Figueredo CM, Gustafsson A
    J. Periodontol., 2006;77(10):1695-700.
    Species: Human
    Sample Types: Gingival Crevicular Fluid (GCF)
  16. Aberrant neutrophil reactions in periodontitis.
    Authors: Figueredo CM, Fischer RG, Gustafsson A
    J. Periodontol., 2005;76(6):951-5.
    Species: Human
    Sample Types: Gingival Crevicular Fluid (GCF)
  17. Midtrimester amniotic fluid matrix metalloproteinase-8 (MMP-8) levels above the 90th percentile are a marker for subsequent preterm premature rupture of membranes.
    Authors: Biggio JR, Ramsey PS, Cliver SP, Lyon MD, Goldenberg RL, Wenstrom KD
    Am. J. Obstet. Gynecol., 2005;192(1):109-13.
    Species: Human
    Sample Types: Amniotic Fluid
  18. Differential modulation of human melanoma cell metalloproteinase expression by alpha2beta1 integrin and CD44 triple-helical ligands derived from type IV collagen.
    Authors: Baronas-Lowell D, Lauer-Fields JL, Borgia JA, Sferrazza GF, Al-Ghoul M, Minond D, Fields GB
    J. Biol. Chem., 2004;279(42):43503-13.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Shifts in biochemical markers associated with wound healing in laryngeal secretions following phonotrauma: a preliminary study.
    Authors: Verdolini K, Rosen CA, Branski RC, Hebda PA
    Ann. Otol. Rhinol. Laryngol., 2003;112(12):1021-5.
    Species: Human
    Sample Types: Tissue Secretion
  20. Antidiabetic PPAR gamma-activator rosiglitazone reduces MMP-9 serum levels in type 2 diabetic patients with coronary artery disease.
    Authors: Marx N, Froehlich J, Siam L, Ittner J, Wierse G, Schmidt A, Scharnagl H, Hombach V, Koenig W
    Arterioscler. Thromb. Vasc. Biol., 2003;23(2):283-8.
    Species: Human
    Sample Types: Serum
  21. Effect of tobacco smoking on neutrophil activity following periodontal surgery.
    Authors: Persson L, Bergstrom J, Gustafsson A
    J. Periodontol., 2003;74(10):1475-82.
    Species: Human
    Sample Types: Gingival Crevicular Fluid (GCF)

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