Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all TRAF-5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TRAF-5
Lys353-Leu557
Accession # O00463

Specificity

Detects human TRAF-5 in direct ELISAs. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) TRAF-3 is observed, and less than 5% cross-reactivity with rhTRAF-2 and rhTRAF-4 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human TRAF‑5 Antibody

TRAF-5 antibody in Human Lymph Node by Immunohistochemistry (IHC-P).

TRAF‑5 in Human Lymph Node.

TRAF-5 was detected in immersion fixed paraffin-embedded sections of human lymph node using Sheep Anti-Human TRAF-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3280) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human TRAF‑5 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymph node

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRAF-5

TRAF-5 (TNF Receptor-Associated Factor 5; also RNF84) is a 60-66 kDa member of the A subfamily of the TRAF family of molecules. It is widely expressed, and serves as an adaptor molecule that links select TNFR family members with downstream signaling elements. In particular, it interacts with LTbR, TNFR2, OX40, CD27, CD40, CD30, RANK and TAJ/TROY, and induces NF kappa B activation. It forms homotrimers and heteromers with TRAF3. Human TRAF5 is 557 amino acids (aa) in length. It contains one RING-type Zn finger region (aa 45-85), two TRAF-type Zn finger regions (aa 127-181 and 182-239), a coiled-coil region (aa 237-342) and a C-terminal protein-protein interaction MATH domain (aa 403-549). There is one potential alternative start site 64 aa upstream of the standard start site. Full-length human TRAF-5 shares 80% aa sequence identity with mouse TRAF-5.

Long Name

TNF Receptor-Associated Factor 5

Alternate Names

RNF84, TRAF5

Entrez Gene IDs

7188 (Human); 22033 (Mouse)

Gene Symbol

TRAF5

UniProt

O00463 (Human)

Additional TRAF-5 Products

Product Documents for Human TRAF‑5 Antibody

Certificate of Analysis

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Product Specific Notices for Human TRAF‑5 Antibody

For research use only

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Protocols

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